Publication:
Carbapenem-Resistant Acinetobacter baumannii: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles

dc.contributor.authorSmitran, Aleksandra (55865631000)
dc.contributor.authorLukovic, Bojana (57189443662)
dc.contributor.authorBozic, LJiljana (57202649173)
dc.contributor.authorJelic, Dijana (23034961300)
dc.contributor.authorJovicevic, Milos (57223044336)
dc.contributor.authorKabic, Jovana (57215669275)
dc.contributor.authorKekic, Dusan (36696225200)
dc.contributor.authorRanin, Jovana (57219407010)
dc.contributor.authorOpavski, Natasa (6507364674)
dc.contributor.authorGajic, Ina (55428924700)
dc.date.accessioned2025-07-02T11:57:18Z
dc.date.available2025-07-02T11:57:18Z
dc.date.issued2023
dc.description.abstractThis study aimed to investigate the biofilm-production ability of carbapenem-resistant Acinetobacter baumannii (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against planktonic and biofilm-embedded CRAB, and the relationship between biofilm production and bacterial genotypes. A total of 111 CRAB isolates were tested for antimicrobial susceptibility, biofilm formation, presence of the genes encoding carbapenemases, and biofilm-associated virulence factors. The antibiofilm effects of disinfectants and SeNPs against CRAB isolates were also tested. The vast majority of the tested isolates were biofilm producers (91.9%). The bap, ompA, and csuE genes were found in 57%, 70%, and 76% of the CRAB isolates, with the csuE being significantly more common among biofilm producers (78.6%) compared to non-biofilm-producing CRAB (25%). The tested disinfectants showed a better antibiofilm effect on moderate and strong biofilm producers than on weak producers (p < 0.01). The SeNPs showed an inhibitory effect against all tested planktonic (MIC range: 0.00015 to >1.25 mg/mL) and biofilm-embedded CRAB, with a minimum biofilm inhibitory concentration of less than 0.15 mg/mL for 90% of biofilm producers. In conclusion, SeNPs might be used as promising therapeutic and medical device coating agents, thus serving as an alternative approach for the prevention of biofilm-related infections. © 2023 by the authors.
dc.identifier.urihttps://doi.org/10.3390/microorganisms11010171
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85146822373&doi=10.3390%2fmicroorganisms11010171&partnerID=40&md5=36f73174690b98c19d9b32505749f465
dc.identifier.urihttps://remedy.med.bg.ac.rs/handle/123456789/11891
dc.subjectbiofilm-associated genes
dc.subjectcarbapenem-resistant Acinetobacter baumannii
dc.subjectdisinfectants
dc.subjectselenium nanoparticles
dc.titleCarbapenem-Resistant Acinetobacter baumannii: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles
dspace.entity.typePublication

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