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Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism analysis of dnaJ gene

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dc.contributor.authorHauschild, Tomasz (23485502800)
dc.contributor.authorStepanović, Srdjan (7004922337)
dc.date.accessioned2025-06-12T23:40:10Z
dc.date.available2025-06-12T23:40:10Z
dc.date.issued2008
dc.description.abstractA PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method was validated by the identification of 23 clinical staphylococcal strains, and the results were compared with those obtained by other genotypic identification methods. A 100% concordance of the results was shown. Therefore, PCR-RFLP analysis of the dnaJ gene is proposed as a reliable and reproducible method for the identification of Staphylococcus spp. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
dc.identifier.urihttps://doi.org/10.1128/JCM.00810-08
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-57349117826&doi=10.1128%2fJCM.00810-08&partnerID=40&md5=97260cbe0d05aba7f74fd6ab5a56724c
dc.identifier.urihttps://remedy.med.bg.ac.rs/handle/123456789/10621
dc.titleIdentification of Staphylococcus spp. by PCR-restriction fragment length polymorphism analysis of dnaJ gene
dspace.entity.typePublication

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