Effects of cis-diamminedichloroplatinum II (cisplatin) on the splenic tissue of rats: A histoquantitative study

Abstract

Histoquantitative methods were applied to study the changes of splenic structure and proportions of splenic tissue compartments in cisplatin-treated (6 mg/kg body wt) male Wistar rats. Six days after treatment the significant reduction of volume density of white pulp was observed, which was due to the very prominent decrease of volume density of follicles. The volume density of periarteriolar lymphatic sheaths did not change substantially. Volume density of the marginal zone was also significantly reduced. A significant increase of volume density of connective tissue was observed. Morphometrical parameters of the follicles were also markedly altered: The number of follicles per mm2 of spleen section area, the numerical density of the follicles in the spleen, and the total number of the follicles were significantly reduced. © 1994 Academic Press, Inc.