Browsing by Author "Tambur, Zoran (6603411139)"

Background/Aim. Bacillus cereus (B. cereus) usually ingested by food can cause two types of diseases: vomiting due to the presence of emetic toxin and diarrheal syndrome, due to the presence of diarrheal toxins. Systemic manifestations can also occur. The severe forms of disease demand antibiotic treatmant. The aim of this study was to determine the differences in antibiotic susceptibility and β-lactamase activity of B. cereus isolates from stools of humans, food and environment. Methods. Identification of B. cereus was performed with selective medium, classical biochemical test and polymerase chain reaction (PCR) with primers specific for bal gene. Thirty isolates from each group were analysed for antibiotic susceptibility using the disk-diffusion assay. Production of β-lactamase was determined by cefinase test, and double-disc method. Results. All strains identified as B. cereus using classical biochemical test, yielded 533 bp fragment with PCR. Isolates from all the three groups were susceptible to imipenem, vancomycin, and erythromycin. All isolates were susceptible to ciprofloxacin but one from the environment. A statistically significant difference between the groups was confirmed to tetracycline and trimethoprim-sulphamethoxazole sensitivity. A total of 28/30 (93.33%) samples from the foods and 25/30 (83.33%) samples from environment were approved sensitive to tetracycline, while 10/30 (33.33%) isolates from stools were sensitive. Opposite to this result, high susceptibility to trimethoprim-sulphamethoxazole was shown in samples from stools (100%), while isolates from foods (63.33%) and from environment (70%) had low susceptibility. All samples produced β-lactamases. Conclusion. The strains of B. cereus from all the three groups showed high rate of sensitivity to most tested antibiotics, except to tetracycline in samples from human stool and to trimethoprim-sulphamethoxazole in samples from food and environment. The production of β-lactamases was confirmed in all the strains. © 2016, Institut za Vojnomedicinske Naucne Informacije/Documentaciju. All rights reserved.

Background/Aim. It is estimated that about 230 million refugees and asylum seekers circulates worldwide. Parasitosis are diagnosed in recent years with increasing frequency both in Europe and other developed countries. International migration of population, as an inalienable part and a result of the process of globalization, has an increasing impact on health of the population of countries through which migrants pass or settle. The aim of this study was to determine the incidence of intestinal parasitic diseases in asylum seekers on the territory of Belgrade. Methods. The study group included 97 asylum seekers from the Centre for Asylum seekers in Obrenovac, in the period December 2013– January 2014. Stool samples were taken less than seven days after arriving to the Center and sent to the Laboratory for Parasitological Diagnosis of the Public Health Institute of Belgrade. Detection of parasites in stool samples was performed by a direct native slide made of fresh sample and direct slide made after stool concentration applying "Mini Parasep" technique. Statistical analysis included application of χ2-test of matching and χ2-test of independence. Results. The study showed that the parasites were detected in 9.3% of cases, in the group of 15–24 years of age. Protozoa were found in 6.2% and helminthes in 3.1% of the samples. Conclusion. Most intestinal parasitosis were found in asylum seekers from Bangladesh. All parasitosis were found in males and the most frequently detected parasite was Giardia lamblia. © 2018, Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.

Background/Aim. Dental status and oral hygiene habits are poor in young population living in deprived socioeconomic conditions. The aim of this research was to ascertain oral hygiene habits in adolescents attending high schools in urban area, determine the incidence of tooth loss at the age of 15–16 years and the gender difference. Methods. The epidemiologi-cal cross-sectional study included 234 randomly selected high school students. The research instruments were questionnaire (focusing on socio-demographic characteristics, habits, attitudes and behavior related to general and oral health) and clinical examination (tooth loss, normative need for prosthodontics restorations and presence of restora-tions). Results. Gender-related data comparison revealed that 32.5% of girls and 8.1% of boys had at least one tooth extracted (p < 0.05). Also, 56.2% of girls and 75.7% of boys brushed their teeth twice a day. Caries complications were identified as the indication for tooth extraction in 82.8% of participants. The data analysis confirmed the correlation between gender and tooth loss as well as treatment needs. Tooth loss was correlated with oral hygiene habits and reasons for dental visits. Treatment need was also affected by the reasons for tooth extraction and the absence of adequate prosthodontics therapy (p < 0.05). Conclusion. Sociodemographic conditions significantly influenced the number of participants with extracted teeth. A prosthodontic treatment need was influenced by the reasons for tooth extraction, adolescents’ knowledge about the importance of adequate treatment and previous unpleasant experience. © 2019, Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.