Browsing by Author "Sulovic, Nenad (23499802400)"

Introduction: HPV is a family of double-stranded DNA viruses that infect the mucous epithelium. Most of these infections regress without consequences; however, the high-risk HPV viral strains can determine a tumor transformation of the epithelium, giving rise to tumor forms such as cervical cancer. The analysis mostly applied to define abnormalities of the membranes is the PAP test and the treatment is reconciled, in most cases through prevention. Alongside the standard protocol, we have determined that a blend of essential oils has an effect in determining the negativization of HPV. Materials and Methods: We made use of the BDORT technique in order to determine the specific percentages of each component of the prepared oil blend. To confirm this, two advanced Molecular Docking software have been applied (Swiss Dock and 1-Click Docking). The mixture of oils was subsequently applied, in the form of vaginal capsules, on a group of HPV (+) positive patients, who were tested for the presence of a virus, before and after treatment, by RT-PCR, Colposcopy, and BDORT to evaluate the state of epithelium before and after the assumption of oil vaginal capsules. Results: The mixture of oils has produced the negativization of HPV, determined by BDORT and confirmed by RT-PCR, in the treated patients. Discussion: We have determined that the blend of essential oils, prepared here, influenced the negativization of the virus, in all the patients treated. Conclusions: The operative efficacy of these oils in the negativization of HPV has been demonstrated. Copyright © 2023 Cognizant Communication Corp.

Introduction: HPV is a family of double-stranded DNA viruses that infect the mucous epithelium. Most of these infections regress without consequences; however, the high-risk HPV viral strains can determine a tumor transformation of the epithelium, giving rise to tumor forms such as cervical cancer. The analysis mostly applied to define abnormalities of the membranes is the PAP test and the treatment is reconciled, in most cases through prevention. Alongside the standard protocol, we have determined that a blend of essential oils has an effect in determining the negativization of HPV. Materials and Methods: We made use of the BDORT technique in order to determine the specific percentages of each component of the prepared oil blend. To confirm this, two advanced Molecular Docking software have been applied (Swiss Dock and 1-Click Docking). The mixture of oils was subsequently applied, in the form of vaginal capsules, on a group of HPV (+) positive patients, who were tested for the presence of a virus, before and after treatment, by RT-PCR, Colposcopy, and BDORT to evaluate the state of epithelium before and after the assumption of oil vaginal capsules. Results: The mixture of oils has produced the negativization of HPV, determined by BDORT and confirmed by RT-PCR, in the treated patients. Discussion: We have determined that the blend of essential oils, prepared here, influenced the negativization of the virus, in all the patients treated. Conclusions: The operative efficacy of these oils in the negativization of HPV has been demonstrated. Copyright © 2023 Cognizant Communication Corp.

INTRODUCTION: Bi-Digital O-Ring Test (BDORT), established and developed by Prof. Y. Omura as non-invasive diagnostic method, may be very useful in fetus gender determination. The aims of the study were: - to evaluate the accuracy of indirect Bi-Digital O-Ring Test in determination of fetus gender and to compare the results obtained with standard methods applied in perinatology; - to compare the value of alpha-fetus protein (AFP) level, obtained by BDORT, with the value of AFP obtained from mother's blood serum (the last one is usually done within triple test together with beta-HCG and estryol) and - to compare the results obtained by BDORT showing pancreatic infection with standard three-hour oral glucose tolerance test (OGTT). MATERIAL AND METHOD: 149 pregnant women were examined by indirect BDORT. The assistant during the test was female. Slides with human sexual chromosomes were used as referent substances: XX and XY. Slides were produced in the laboratory for Genetics of Mount Sinai Hospital, NYU. 15 pregnant women, between 6th and 11th week of gestational age, were examined prior chorionic villi biopsy and genetic analysis. 134 pregnant women, between 18th and 36th week of gestation, were examined by ultrasonography in order to determine fetus gender. The results obtained from indirect BDORT were compared with the fetus gender following delivery. On the other side, reference AFP slides were used and applied over the uterus and liver of pregnant women. These results were compared with the results of serum levels of AFP. Blood for analysis was collected while women were between 14th and 16th gestational week of pregnancy. Also, in patients whom indirect BDORT revealed pancreatic infection, reference slides with acetylcholine (ACh) were applied; the patients showing ACh resonance at 100pg (BDORT Unit) or less, were referred to lab to perform 3-hour OGTT with 75g of glucose. RESULTS & DISCUSSION: The same results of gender determination were obtained by using both BDORT, on one side, and by chorionic villi biopsy and genetic analysis, on the other in vast majority of cases. The diagnosis was missed in three cases and some peculiar information was obtained in five cases. When test is performed by using both slides (XX and XY), the test results were negative. The same results of gender determination by using BDORT on one side and standard methods on the other, were obtained in 94.63%. Reference slides with AFP showed the level of 4ng (BDORT Unit) which is connected with normal pregnancy. In 2 cases out of 50 pregnant women, AFP reference slides resonated over the liver at increased level of 70 and 90ng (BDORT Unit), while over the uterus, level was 4ng (BDORT Unit) in both cases. Also, in the same patients, BDORT showed liver infection with CMV. Blood analysis performed in these two patients, revealed increased level of IgG CMV which was not the case with IgM. Ultrasonography showed normal morphology of fetuses; the results of amniocentesis and genetic analysis were within physiological frames and pregnancies were continued. The deliveries were normal, babies were healthy with somewhat less body mass. BDORT showed infection of pancreas in 9 out of 128 pregnant women. Since pancreatic infection results in lowering of ACh level over pancreas, reference ACh slides also were used. The resonance with ACh slides was obtained at 100pg (BDORT Unit) and less. Those patients referred to lab where 3-hour lasting OGTT was performed with 75g of glucose. The test was positive revealing glucose intolerance in 8 patients (89%). In one patient, BDORT revealed asymptomatic infection with Toxoplasma gondii. This result was confirmed by routine blood analysis showing increased IgM level. In two patients, BDORT revealed infection with Chlamydia trachomatis. Cervical smear and serological analysis showed no presence of IgG, IgM, and IgA. However, Chlamydia trachomatis was obtained and confirmed in amniotic fluid following amniocentesis. This finding was put in connection with the presence of chronic endometritis prior pregnancy in those two women. CONCLUSION: This study results indicate that indirect BDORT may be applied as a screening method to determine fetus gender. This fact will raise the other issue, namely, whether indirect BDORT may be applied in screening of various genetic syndromes and genetic diseases when slides, with the proper referent substances, are used. Also, the finding of non-specific hepatitis caused by CMV, can be possible cause of increased level of AFP during pregnancy. AFP at the level of 4ng over the uterus of pregnant woman shows that there is no fetus anomaly. Therefore, well trained physicians may screen for possible changes in fetus by using BDORT and AFP level. Regarding pancreatic infection and ACh, BDORT showed that, besides normal fasting blood glucose level, certain changes in sugar metabolism may be present and, whether ACh is lowered, 3-hour OGTT is recommended. By referring to lab patients with normal fasting blood glucose level and lowered ACh level, physicians may reveal patients with glucose intolerance and preventive measures may be applied (appropriate diet, physical activities, more frequent check-ups of woman and fetus etc. Generally, by using indirect BDORT as non-invasive and safe method in pregnant women, physicians may detect asymptomatic infections (such is Toxoplasma gondii infection) and changes (such is glucose intolerance) which, in turn, may result in adequate approach to each patient. This will enable a wide range of preventive measures to be undertaken and, in that way, the onset of certain diseases will be prevented.

INTRODUCTION: Bi-Digital O-Ring Test (BDORT), established and developed by Prof. Y. Omura as non-invasive diagnostic method, may be very useful in fetus gender determination. The aims of the study were: - to evaluate the accuracy of indirect Bi-Digital O-Ring Test in determination of fetus gender and to compare the results obtained with standard methods applied in perinatology; - to compare the value of alpha-fetus protein (AFP) level, obtained by BDORT, with the value of AFP obtained from mother's blood serum (the last one is usually done within triple test together with beta-HCG and estryol) and - to compare the results obtained by BDORT showing pancreatic infection with standard three-hour oral glucose tolerance test (OGTT). MATERIAL AND METHOD: 149 pregnant women were examined by indirect BDORT. The assistant during the test was female. Slides with human sexual chromosomes were used as referent substances: XX and XY. Slides were produced in the laboratory for Genetics of Mount Sinai Hospital, NYU. 15 pregnant women, between 6th and 11th week of gestational age, were examined prior chorionic villi biopsy and genetic analysis. 134 pregnant women, between 18th and 36th week of gestation, were examined by ultrasonography in order to determine fetus gender. The results obtained from indirect BDORT were compared with the fetus gender following delivery. On the other side, reference AFP slides were used and applied over the uterus and liver of pregnant women. These results were compared with the results of serum levels of AFP. Blood for analysis was collected while women were between 14th and 16th gestational week of pregnancy. Also, in patients whom indirect BDORT revealed pancreatic infection, reference slides with acetylcholine (ACh) were applied; the patients showing ACh resonance at 100pg (BDORT Unit) or less, were referred to lab to perform 3-hour OGTT with 75g of glucose. RESULTS & DISCUSSION: The same results of gender determination were obtained by using both BDORT, on one side, and by chorionic villi biopsy and genetic analysis, on the other in vast majority of cases. The diagnosis was missed in three cases and some peculiar information was obtained in five cases. When test is performed by using both slides (XX and XY), the test results were negative. The same results of gender determination by using BDORT on one side and standard methods on the other, were obtained in 94.63%. Reference slides with AFP showed the level of 4ng (BDORT Unit) which is connected with normal pregnancy. In 2 cases out of 50 pregnant women, AFP reference slides resonated over the liver at increased level of 70 and 90ng (BDORT Unit), while over the uterus, level was 4ng (BDORT Unit) in both cases. Also, in the same patients, BDORT showed liver infection with CMV. Blood analysis performed in these two patients, revealed increased level of IgG CMV which was not the case with IgM. Ultrasonography showed normal morphology of fetuses; the results of amniocentesis and genetic analysis were within physiological frames and pregnancies were continued. The deliveries were normal, babies were healthy with somewhat less body mass. BDORT showed infection of pancreas in 9 out of 128 pregnant women. Since pancreatic infection results in lowering of ACh level over pancreas, reference ACh slides also were used. The resonance with ACh slides was obtained at 100pg (BDORT Unit) and less. Those patients referred to lab where 3-hour lasting OGTT was performed with 75g of glucose. The test was positive revealing glucose intolerance in 8 patients (89%). In one patient, BDORT revealed asymptomatic infection with Toxoplasma gondii. This result was confirmed by routine blood analysis showing increased IgM level. In two patients, BDORT revealed infection with Chlamydia trachomatis. Cervical smear and serological analysis showed no presence of IgG, IgM, and IgA. However, Chlamydia trachomatis was obtained and confirmed in amniotic fluid following amniocentesis. This finding was put in connection with the presence of chronic endometritis prior pregnancy in those two women. CONCLUSION: This study results indicate that indirect BDORT may be applied as a screening method to determine fetus gender. This fact will raise the other issue, namely, whether indirect BDORT may be applied in screening of various genetic syndromes and genetic diseases when slides, with the proper referent substances, are used. Also, the finding of non-specific hepatitis caused by CMV, can be possible cause of increased level of AFP during pregnancy. AFP at the level of 4ng over the uterus of pregnant woman shows that there is no fetus anomaly. Therefore, well trained physicians may screen for possible changes in fetus by using BDORT and AFP level. Regarding pancreatic infection and ACh, BDORT showed that, besides normal fasting blood glucose level, certain changes in sugar metabolism may be present and, whether ACh is lowered, 3-hour OGTT is recommended. By referring to lab patients with normal fasting blood glucose level and lowered ACh level, physicians may reveal patients with glucose intolerance and preventive measures may be applied (appropriate diet, physical activities, more frequent check-ups of woman and fetus etc. Generally, by using indirect BDORT as non-invasive and safe method in pregnant women, physicians may detect asymptomatic infections (such is Toxoplasma gondii infection) and changes (such is glucose intolerance) which, in turn, may result in adequate approach to each patient. This will enable a wide range of preventive measures to be undertaken and, in that way, the onset of certain diseases will be prevented.

This study presents a comparative analysis of molecular docking data, focusing on the binding interactions of the natural compounds apigenin and luteolin with the proteins TP-53, pRb, and APOBEC, in comparison to conventional pharmacological ligands. Advanced bioinformatics techniques were employed to evaluate and contrast binding energies, showing that apigenin and luteolin demonstrate significantly higher affinities for TP-53, pRb, and APOBEC, with binding energies of −6.9 kcal/mol and −6.6 kcal/mol, respectively. These values suggest strong potential for therapeutic intervention against HPV-16. Conventional ligands, by comparison, exhibited lower affinities, with energies ranging from −4.5 to −5.5 kcal/mol. Additionally, protein–protein docking simulations were performed to assess the interaction between HPV-16 E6 oncoprotein and tumor suppressors TP-53 and pRb, which revealed high binding energies around −976.7 kcal/mol, indicative of their complex interaction. A conversion formula was applied to translate these protein–protein interaction energies to a comparable scale for non-protein interactions, further underscoring the superior binding potential of apigenin and luteolin. These findings highlight the therapeutic promise of these natural compounds in preventing HPV-16-induced oncogenesis, warranting further experimental validation for clinical applications. © 2024 by the authors.

This study presents a comparative analysis of molecular docking data, focusing on the binding interactions of the natural compounds apigenin and luteolin with the proteins TP-53, pRb, and APOBEC, in comparison to conventional pharmacological ligands. Advanced bioinformatics techniques were employed to evaluate and contrast binding energies, showing that apigenin and luteolin demonstrate significantly higher affinities for TP-53, pRb, and APOBEC, with binding energies of −6.9 kcal/mol and −6.6 kcal/mol, respectively. These values suggest strong potential for therapeutic intervention against HPV-16. Conventional ligands, by comparison, exhibited lower affinities, with energies ranging from −4.5 to −5.5 kcal/mol. Additionally, protein–protein docking simulations were performed to assess the interaction between HPV-16 E6 oncoprotein and tumor suppressors TP-53 and pRb, which revealed high binding energies around −976.7 kcal/mol, indicative of their complex interaction. A conversion formula was applied to translate these protein–protein interaction energies to a comparable scale for non-protein interactions, further underscoring the superior binding potential of apigenin and luteolin. These findings highlight the therapeutic promise of these natural compounds in preventing HPV-16-induced oncogenesis, warranting further experimental validation for clinical applications. © 2024 by the authors.

Objective: To measure fetal and maternal plasma homocysteine (Hcy) concentrations in uncomplicated pregnancies. Methods: Paired maternal venous and fetal umbilical cord blood (n = 81) samples were evaluated for plasma Hcy and vitamin B12 levels, in addition to eight neonatal umbilical cord blood samples obtained immediately following delivery. Results: Both fetal and maternal Hcy concentrations were positively correlated with advancing gestational age (ρ = 0.44, p < 0.0001; and ρ = 0.27, p < 0.05, respectively). Fetal plasma Hcy concentrations [2.2 μmol/l (IQR: 2.0-3.2)] were significantly lower than both neonatal umbilical vein [5.0 μmol/l (IQR: 4.4-6.5); p < 0.001] and maternal plasma Hcy levels [4.4 μmo/l (IQR: 3.4-5.4); p < 0.001]. In addition, Hcy values at term were higher in the umbilical vein compared with the umbilical artery [5.0 μmol/l (IQR: 3.4-5.4) versus 4.2 μmol/l (IQR: 3.7-5.5), respectively; p = 0.016]. Significant correlation was noted and between fetal and maternal Hcy levels (ρ = 0.50, p < 0.0001), while fetal Hcy was negatively correlated with maternal B12 concentrations (ρ = -0.32, p < 0.001). Conclusions: Fetal Hcy levels were significantly lower than maternal and neonatal levels and correlated with gestational age across the second half of pregnancy. © 2014 Informa UK Ltd. All rights reserved.

Introduction: Organisms are constantly immersed in an ocean of electromagnetic waves which cause different effects on the body. At the end of 1980, it was determined that the frequency of 6.66 Hz is responsible for anxiety-depressive states and that all subjects who manifest such disturbances vibrate on this frequency. Materials and Methods: The BDORT technique was applied to evaluate parameters associated with the subject’s status (Sirtuin-1, TERT, TP-53, Zoloft, Thromboxane B2, Acetylcholine, DMAE, Thymus Gland). Furthermore, the GAP-7 and HAMD psycho-aptitude tests were applied to evaluate the subject’s anxiety-depressive level. At the end of the evaluation, the subject was treated with the OPFT (Opposite Phase Frequency Treatment), i.e., with the counter-phase frequency of -6.66 Hz, in order to cancel the 6.66 Hz frequency. The treatment was performed by listening, with headphones, to the specific anti-frequency, elaborated thanks to the combined use of artificial intelligence software. Results: Patients treated with the OPFT showed an increase, measured by BDORT, of specific biological parameters and a decrease in Thromboxane B2, in accordance with expectations, due to the balance between the sympathetic and parasympathetic systems. The data obtained, on the specific parameters, showed a statistically significant correlation (p < 0.005 ** and p < 0.001 ***). Discussion: The increase in the value of parameters Acetylcholine and the decrease in Thromboxane B2, was the basis of the alleviation of anxiety-depressive symptoms. As a corollary of them, there was an increase in parameters such as Sirtuin-1, TERT, and TP-53. Conclusions: The OPFT technique proved to be effective in reducing anxiety-depressive symptoms in all treated subjects. Copyright ©2023 Cognizant Communication Corp. Printed in the USA.

Introduction: Organisms are constantly immersed in an ocean of electromagnetic waves which cause different effects on the body. At the end of 1980, it was determined that the frequency of 6.66 Hz is responsible for anxiety-depressive states and that all subjects who manifest such disturbances vibrate on this frequency. Materials and Methods: The BDORT technique was applied to evaluate parameters associated with the subject’s status (Sirtuin-1, TERT, TP-53, Zoloft, Thromboxane B2, Acetylcholine, DMAE, Thymus Gland). Furthermore, the GAP-7 and HAMD psycho-aptitude tests were applied to evaluate the subject’s anxiety-depressive level. At the end of the evaluation, the subject was treated with the OPFT (Opposite Phase Frequency Treatment), i.e., with the counter-phase frequency of -6.66 Hz, in order to cancel the 6.66 Hz frequency. The treatment was performed by listening, with headphones, to the specific anti-frequency, elaborated thanks to the combined use of artificial intelligence software. Results: Patients treated with the OPFT showed an increase, measured by BDORT, of specific biological parameters and a decrease in Thromboxane B2, in accordance with expectations, due to the balance between the sympathetic and parasympathetic systems. The data obtained, on the specific parameters, showed a statistically significant correlation (p < 0.005 ** and p < 0.001 ***). Discussion: The increase in the value of parameters Acetylcholine and the decrease in Thromboxane B2, was the basis of the alleviation of anxiety-depressive symptoms. As a corollary of them, there was an increase in parameters such as Sirtuin-1, TERT, and TP-53. Conclusions: The OPFT technique proved to be effective in reducing anxiety-depressive symptoms in all treated subjects. Copyright ©2023 Cognizant Communication Corp. Printed in the USA.