Browsing by Author "Stevanovic, Strahinja (57189646996)"

In the published article, there was an error in the Author list, and author Elise Courtot was erroneously excluded. The corrected author list appears below. “Maja Stojković1, Zoran Todorović1, Dragana Protić1, Strahinja Stevanovic2, Dragana Medić3, Claude L. Charvet4, Elise Courtot4, Djordje S. Marjanović3, Jelena Nedeljković Trailović5, Saša M. Trailović3,*” The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. Copyright © 2024 Stojković, Todorović, Protic, Stevanovic, Medić, L. Charvet, Courtot, Marjanović, Nedeljković Trailović and Trailović.

In the published article, there was an error in the Author list, and author Elise Courtot was erroneously excluded. The corrected author list appears below. “Maja Stojković1, Zoran Todorović1, Dragana Protić1, Strahinja Stevanovic2, Dragana Medić3, Claude L. Charvet4, Elise Courtot4, Djordje S. Marjanović3, Jelena Nedeljković Trailović5, Saša M. Trailović3,*” The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. Copyright © 2024 Stojković, Todorović, Protic, Stevanovic, Medić, L. Charvet, Courtot, Marjanović, Nedeljković Trailović and Trailović.

The control of parasitic nematode infections relies mostly on anthelmintics. The potential pharmacotherapeutic application of phytochemicals, in order to overcome parasite resistance and enhance the effect of existing drugs, is becoming increasingly important. The antinematodal effects of carveol was tested on the free-living nematode Caenorhabditis elegans and the neuromuscular preparation of the parasitic nematode Ascaris suum. Carveol caused spastic paralysis in C. elegans. In A. suum carveol potentiated contractions induced by acetylcholine (ACh) and this effect was confirmed with two-electrode voltage-clamp electrophysiology on the A. suum nicotinic ACh receptor expressed in Xenopus oocytes. However, potentiating effect of carveol on ACh-induced contractions was partially sensitive to atropine, indicates a dominant nicotine effect but also the involvement of some muscarinic structures. The effects of carveol on the neuromuscular system of mammals are also specific. In micromolar concentrations, carveol acts as a non-competitive ACh antagonist on ileum contractions. Unlike atropine, it does not change the EC50 of ACh, but reduces the amplitude of contractions. Carveol caused an increase in Electrical Field Stimulation-evoked contractions of the isolated rat diaphragm, but at higher concentrations it caused an inhibition. Also, carveol neutralized the mecamylamine-induced tetanic fade, indicating a possibly different pre- and post-synaptic action at the neuromuscular junction. Copyright © 2024 Stojković, Todorović, Protic, Stevanovic, Medić, Charvet, Marjanović, Nedeljković Trailović and Trailović.

The control of parasitic nematode infections relies mostly on anthelmintics. The potential pharmacotherapeutic application of phytochemicals, in order to overcome parasite resistance and enhance the effect of existing drugs, is becoming increasingly important. The antinematodal effects of carveol was tested on the free-living nematode Caenorhabditis elegans and the neuromuscular preparation of the parasitic nematode Ascaris suum. Carveol caused spastic paralysis in C. elegans. In A. suum carveol potentiated contractions induced by acetylcholine (ACh) and this effect was confirmed with two-electrode voltage-clamp electrophysiology on the A. suum nicotinic ACh receptor expressed in Xenopus oocytes. However, potentiating effect of carveol on ACh-induced contractions was partially sensitive to atropine, indicates a dominant nicotine effect but also the involvement of some muscarinic structures. The effects of carveol on the neuromuscular system of mammals are also specific. In micromolar concentrations, carveol acts as a non-competitive ACh antagonist on ileum contractions. Unlike atropine, it does not change the EC50 of ACh, but reduces the amplitude of contractions. Carveol caused an increase in Electrical Field Stimulation-evoked contractions of the isolated rat diaphragm, but at higher concentrations it caused an inhibition. Also, carveol neutralized the mecamylamine-induced tetanic fade, indicating a possibly different pre- and post-synaptic action at the neuromuscular junction. Copyright © 2024 Stojković, Todorović, Protic, Stevanovic, Medić, Charvet, Marjanović, Nedeljković Trailović and Trailović.