Browsing by Author "Stanojevic, Aleksandar (6507614733)"

Previous studies have revealed that the European Roma share close genetic, linguistic and cultural similarities with Indian populations despite their disparate geographical locations and divergent demographic histories. In this study, we report for the first time Y-chromosome distributions in three Roma collections residing in Belgrade, Vojvodina and Kosovo. Eighty-eight Y-chromosomes were typed for 14 SNPs and 17 STRs. The data were subsequently utilized for phylogenetic comparisons to pertinent reference collections available from the literature. Our results illustrate that the most notable difference among the three Roma populations is in their opposing distributions of haplogroups H and E. Although the Kosovo and Belgrade samples exhibit elevated levels of the Indian-specific haplogroup H-M69, the Vojvodina collection is characterized almost exclusively by haplogroup E-M35 derivatives, most likely the result of subsequent admixture events with surrounding European populations. Overall, the available data from Romani groups points to different levels of gene flow from local populations. © 2010 Wiley-Liss, Inc.

Previous studies have revealed that the European Roma share close genetic, linguistic and cultural similarities with Indian populations despite their disparate geographical locations and divergent demographic histories. In this study, we report for the first time Y-chromosome distributions in three Roma collections residing in Belgrade, Vojvodina and Kosovo. Eighty-eight Y-chromosomes were typed for 14 SNPs and 17 STRs. The data were subsequently utilized for phylogenetic comparisons to pertinent reference collections available from the literature. Our results illustrate that the most notable difference among the three Roma populations is in their opposing distributions of haplogroups H and E. Although the Kosovo and Belgrade samples exhibit elevated levels of the Indian-specific haplogroup H-M69, the Vojvodina collection is characterized almost exclusively by haplogroup E-M35 derivatives, most likely the result of subsequent admixture events with surrounding European populations. Overall, the available data from Romani groups points to different levels of gene flow from local populations. © 2010 Wiley-Liss, Inc.

To develop in house protocol for DNA analyses of contact traces, we conducted a series of experiments using low copy number (LCN-PCR) [P. Gill, Application of low copy number DNA profiling, Croatian Med. J. 42(3)(2001) 229-232] amplification of DNA isolated from touched objects. In each experiment, touched objects were swabbed using double swab technique, DNA was extracted by organic extraction protocol, and pooled DNA extracts from both wet and dry swabs were amplified using AmpFlSTR Identifiler kit in 34-cycle PCR. In the first part of this study, seven volunteers held sterile plastic tubes for 10 s, 15 min after washing their hands. From seven tested subjects, we recovered three full and four partial profiles. One of the partial profiles differed from volunteer's reference profile. In the second experiment, volunteers held each other's ankles in order to investigate success of DNA analyses from material transferred by interpersonal skin contact. Mixtures of both persons were obtained from all swabs, and the ratio of each person in the isolate, depended both on shedding status, as well as on hand dominance. The third part of this research tested the effect of the period after the deposition on both quantity and quality of DNA extract. Full DNA profiles were obtained even after 24 h since deposition for two good shedders. In conclusion, LCN-PCR technology provides a valuable approach in DNA typing of trace amounts of biological material, left on even shortly touched objects. © 2008.

To develop in house protocol for DNA analyses of contact traces, we conducted a series of experiments using low copy number (LCN-PCR) [P. Gill, Application of low copy number DNA profiling, Croatian Med. J. 42(3)(2001) 229-232] amplification of DNA isolated from touched objects. In each experiment, touched objects were swabbed using double swab technique, DNA was extracted by organic extraction protocol, and pooled DNA extracts from both wet and dry swabs were amplified using AmpFlSTR Identifiler kit in 34-cycle PCR. In the first part of this study, seven volunteers held sterile plastic tubes for 10 s, 15 min after washing their hands. From seven tested subjects, we recovered three full and four partial profiles. One of the partial profiles differed from volunteer's reference profile. In the second experiment, volunteers held each other's ankles in order to investigate success of DNA analyses from material transferred by interpersonal skin contact. Mixtures of both persons were obtained from all swabs, and the ratio of each person in the isolate, depended both on shedding status, as well as on hand dominance. The third part of this research tested the effect of the period after the deposition on both quantity and quality of DNA extract. Full DNA profiles were obtained even after 24 h since deposition for two good shedders. In conclusion, LCN-PCR technology provides a valuable approach in DNA typing of trace amounts of biological material, left on even shortly touched objects. © 2008.

With the exception of exhumations of mass graves in Latin America, forensic dentists and odontologists are rarely involved in the examination of mortal remains recovered from mass graves. The cited reason is often that " there are no dental records-so what is the point"? In this presentation we review the published accounts of examination of remains arising from the conflict in the former Yugoslavia between 1991 and 1999 in which dental examinations are reported. There are roughly 30,000 missing persons of which more than 15,000 mortal remains have been identified, mostly based on DNA. There are 9 sources which describe postmortem dental examinations of 3919 sets of remains; of these, 23% were purported to have been identified specifically from dental information. Of the 8100 listed missing persons from the Srebrenica mass killings in 1995, we located 600 dental records. A sample of 263 charts was examined for information about first molar treatment as we are concerned that dental charting of individuals who lose their first molars may be incorrectly done if allowance is not made for mesial drift of the remaining molars. We found that of all the first molar extractions that are ever going to occur according to these dental charts, 63% have taken place by age 18. The majority of extracted first molars have a functional age of 17 years. We observe that an adult's remains from Srebrenica usually have only second and third molars, which have often drifted forward to occupy the position of the first molar creating the appearance of third molar agenesis. We conclude: that, since dental identifications of victims in mass graves and mass disasters is the exception rather than the rule, even in the absence of DNA-based identifications, international forensic odontologists have an ethical obligation to become more involved in examination of mass grave victims, that there must be more determined searches for antemortem dental records; that local dentists should be approached to participate in the examination of remains and lastly that dental examination and charting by anthropologists and pathologists may be grossly inaccurate. Furthermore, even in the absence of dental records, there is significant information about the individual to be obtained by an oral biologist since many families have useful memories about the oral status of their loved ones who went missing. © 2010 Elsevier Ireland Ltd.