Browsing by Author "Stankovic, Marija (56954542900)"

The aim of the study was to examine alteration and possible application of fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely inflamed tissue. Acute inflammation was induced by injection of turpentine oil into the right and left hind limb muscles of mice, whereas control animals received intramuscular saline injection. After 12 h, animals were anesthetised and treated muscles collected. The tissue was stained by hematoxylin and eosin, digital micrographs produced, enabling determination of fractal dimension of the cells, angular second moment and correlation of studied tissue. Histopathological analysis showed presence of inflammatory infiltrate and tissue damage in inflammatory group, whereas tissue structure in control group was preserved, devoid of inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of treated tissue in inflammatory group decreased in comparison to the control group. In this study, we were first to observe and report that fractal dimension of the cells, angular second moment, and correlation were reduced in acutely inflamed tissue, indicating loss of overall complexity of the cells in the tissue, the tissue uniformity and structure regularity. Fractal dimension, angular second moment and correlation could be useful methods for quantification of structural changes in acute inflammation. Lay Description: The aim of this study was to examine alteration, and possible application of mathematical parameters fractal dimension, angular second moment, and correlation for quantification of structural changes in acutely-inflamed tissue. An acute inflammation was induced by injection of turpentine oil into mice muscles, whereas control group received intramuscular injection of saline. After 12 h animals were anesthetized, and treated muscles were collected. The tissue was stained, and photos of the tissue were made. Mathematical parameters, namely fractal dimension, angular second moment, and correlation of the tissue photo, were determined by computer program. Standard histopathological analysis showed that inflammatory infiltrate and tissue damage were present in inflammatory group, whereas tissue structure in control group was preserved, and without inflammatory infiltrate. Fractal dimension of the cells, angular second moment and correlation of the treated tissue in inflammatory group decreased, when compared to control group. In this study we reported, for the first time, that fractal dimension of the cells, angular second moment, and correlation had decreased in acutely-inflamed tissue, indicating loss of overall complexity of cells in tissue, tissue uniformity, and structure regularity. Fractal dimension, angular second moment, and correlation could be useful methods for quantification of structural changes in acute inflammation. © 2016 Royal Microscopical Society.

Uveitis is a group of inflammatory ocular diseases, primarily affecting uveal tract. Although the pathogenesis of endogenous uveitis is not completely understood, it is well established that immune mechanisms are involved. Cytokines are soluble proteins that function as mediators of immune responses and understanding their role in the development of endogenous uveitis could contribute to elucidation of the etiopathogenesis of this disease. In this review article the role of the most important cytokines is analyzed based on data from the studies with experimental animal models or patients with endogenous uveitis. Cytokines, such as interleukin-1, interleukin-2, interleukin-6 or tumor necrosis factor-alpha, have clear pro-inflammatory role in endogenous uveitis, while protective, anti-inflammatory role is ascribed to interleukin-10 and transforming growth factor-beta. Due to scarce and often contradictory results, the roles of interleukin-4, interleukin-5, interleukin-12, interleukin-17, interleukin-23, interferon-gamma and other cytokines in the pathogenesis of endogenous uveitis have not been unambiguously defined. Further studies are needed to delineate the precise role of these cytokines in endogenous veitis, which would open new possibilities in the treatment of this disease and prevention of its complications that can lead to vision loss.