Browsing by Author "Rosic, Gvozden (6506024209)"

The aim of this study was to determine the mechanism of transport of 14C-thiamin in the hearts of healthy (non-alcoholic) and chronically alcoholic guinea pigs. We used the single-pass, paired-tracer dilution method on isolated and retrogradely perfused guinea pig hearts. The maximal cellular uptake (Umax) and total cellular uptake (Utot) of 14C-thiamin were determined under control conditions and under influence of possible modifiers. We tested how the presence of unlabeled thiamin, metabolic inhibitors, or absence of sodium ions influence the transport of 14C-thiamin. The results of our experiments show that the transport of 14C-thiamin is specific and energy-dependent and that its properties are significantly changed under the influence of chronic alcoholism. The latter effect occurs by increase in both Umax and Utot, as a manifestation of a compensatory mechanism in thiamin deficiency. © 2008 NRC.

The aim of this study was to determine the mechanism of transport of 14C-thiamin in the hearts of healthy (non-alcoholic) and chronically alcoholic guinea pigs. We used the single-pass, paired-tracer dilution method on isolated and retrogradely perfused guinea pig hearts. The maximal cellular uptake (Umax) and total cellular uptake (Utot) of 14C-thiamin were determined under control conditions and under influence of possible modifiers. We tested how the presence of unlabeled thiamin, metabolic inhibitors, or absence of sodium ions influence the transport of 14C-thiamin. The results of our experiments show that the transport of 14C-thiamin is specific and energy-dependent and that its properties are significantly changed under the influence of chronic alcoholism. The latter effect occurs by increase in both Umax and Utot, as a manifestation of a compensatory mechanism in thiamin deficiency. © 2008 NRC.

Background and Objectives: This study analyzed the frequency of factors influencing the course and outcomes of out-of-hospital cardiac arrest (OHCA) in Serbia and the prediction of pre-hospital outcomes and survival. Materials and Methods: Data were collected during the period from 1 October 2014, to 31 September 2023, according to the protocol of the EuReCa_One study (clinical trial ID number NCT02236819). Results: Overall 9303 OHCA events were registered with a median age of 71 (IQR 61–81) years and 59.7% of them being males. The annual OHCA incidence was 85.60 ± 20.73/100,000. Within all bystander-witnessed cases, bystander-initiated cardiopulmonary resuscitation in 15.3%. Within the resuscitation-initiated group, return-of-spontaneous circulation (ROSC) on scene (any ROSC) was present in 1037/4053 cases (25.6%) and ROSC on admission to the nearest hospital in 792/4053 cases (19.5%), while 201/4053 patients survived to hospital discharge (5.0%). Predictive potential on pre-hospital outcomes was shown by several factors. Also, of all patients having any ROSC, 89.2% were admitted to the hospital alive. The probability of any ROSC dropped below 50% after 17 min passed after the emergency call and 10 min after the EMS scene arrival. These time intervals were significantly associated with survival to hospital discharge (p < 0.001). Five-minute time intervals between both emergency calls and any ROSC and EMS scene arrival and any ROSC also had a significant predictive potential for survival to hospital discharge (p < 0.001, HR 1.573, 95% CI 1.303–1.899 and p = 0.017, HR 1.184, 95% CI 1.030–1.361, respectively). Conclusions: A 10-min time on scene to any ROSC is a crucial time-related factor for achieving any ROSC, and indirectly admission ROSC and survival to hospital discharge, and represents a golden time interval spent on scene in the management of OHCA patients. A similar effect has a time interval of 17 min from an emergency call. Further investigations should be focused on factors influencing these time intervals, especially time spent on scene. © 2024 by the authors.