Browsing by Author "Radojevic-Skodric, S. (15726145200)"

Objectives: Secretory IgA is predominant immunoglobulin in secretions of the mucosal immune system. Low concentration of salivary IgA is associated with a higher risk of respiratory tract infections episodes in athletes during the training season. The purpose of this study was to determine whether orange-flavored whey based drink has beneficial effect on level of IgA in saliva after the training. Methods: Twenty healthy, young karate players participated in this study. They were divided into two groups. One group rinsed a mouth with 20 ml of liquid whey, two times in 30-min period: 15 and 30 min after training. Second group rinsed a mouth with 20 ml of whey based orange flavored soft drink, at the same time. Saliva was collected before the training, just after the training and after application of fluids. Results: We observed decrease in salivary flow after physical activity. The salivary flow was higher after the application of flavored drink compared to salivary flow after the application of whey. The absolute concentration of slgA and slgA secretion rate decreased just after exercise compared to pre exercise values. Application of whey elevated slgA levels on day 1, while application of flavored drink caused increasing in slgA levels on day 3. In all other cases slgA level was decreasing even after applied stimuli. Conclusions: The exercise induces decreasing in salivary flow, slgA absolute concentration and slgA secretion rate. Application of fluid whey and flavored whey-based drink elevated salivary flow, but had little effect on absolute concentration of slgA and slgA secretion rate in young karate players.

Objectives: Secretory IgA is predominant immunoglobulin in secretions of the mucosal immune system. Low concentration of salivary IgA is associated with a higher risk of respiratory tract infections episodes in athletes during the training season. The purpose of this study was to determine whether orange-flavored whey based drink has beneficial effect on level of IgA in saliva after the training. Methods: Twenty healthy, young karate players participated in this study. They were divided into two groups. One group rinsed a mouth with 20 ml of liquid whey, two times in 30-min period: 15 and 30 min after training. Second group rinsed a mouth with 20 ml of whey based orange flavored soft drink, at the same time. Saliva was collected before the training, just after the training and after application of fluids. Results: We observed decrease in salivary flow after physical activity. The salivary flow was higher after the application of flavored drink compared to salivary flow after the application of whey. The absolute concentration of slgA and slgA secretion rate decreased just after exercise compared to pre exercise values. Application of whey elevated slgA levels on day 1, while application of flavored drink caused increasing in slgA levels on day 3. In all other cases slgA level was decreasing even after applied stimuli. Conclusions: The exercise induces decreasing in salivary flow, slgA absolute concentration and slgA secretion rate. Application of fluid whey and flavored whey-based drink elevated salivary flow, but had little effect on absolute concentration of slgA and slgA secretion rate in young karate players.

Purpose: It is known that expression disorders of cell cycle regulators play an important role in the development and prognosis of various malignant tumors. Cyclin expression changes during the cell cycle. This work aimed to analyse the expression of cyclin E in transitional cell carcinoma (TCC) and also to compare the expression of cyclin E with tumor stage and histological grade as well as to determine possible existence of differences in the expression of cyclin E in TCCs of the upper and lower urothelium. Methods: Twenty-four cases of TCC of the urinary tract were retrospectively analysed (6 cancers of the renal pelvis, 2 of the ureter and 15 of the bladder; 4 were infiltrative). Immunohistochemical staining for cyclin E of the analysed transitional cancer cells was assessed semiquantitatively: diffuse cyclin E expression + + + (> 50% of all cells), expression in larger groups of cells: + + (up to 50% of all cells), expression in individual cells or small cell clusters: + (<10% of all cells), and absence of expression. Tumor stage was based on clinical and morphological criteria. WHO classification (Lyon 2004) was used for determination of the histological grade. Results: Non-parametric Spearman's correlation showed that there was no statistically significant correlation between tumor stage and expression of cyclin E(ρ=-0331, p>0.05). Also, no statistically significant correlation between grade and the expression of cyclin E(ρ=-0077, p>0.05) was found. x 2 test results showed no statistically significant difference (x 2 = 2.136, p=0.775) in the expression of cyclin E between upper and lower urothelium. Conclusion: This study showed non significant decreased expression of cyclin E with poor differentiation, muscle invasion and upper/lower urothelium. Expression of cyclin E decreased with increasing histological grade and stage of the tumor. © 2011 Zerbinis Medical Publications.

Purpose: It is known that expression disorders of cell cycle regulators play an important role in the development and prognosis of various malignant tumors. Cyclin expression changes during the cell cycle. This work aimed to analyse the expression of cyclin E in transitional cell carcinoma (TCC) and also to compare the expression of cyclin E with tumor stage and histological grade as well as to determine possible existence of differences in the expression of cyclin E in TCCs of the upper and lower urothelium. Methods: Twenty-four cases of TCC of the urinary tract were retrospectively analysed (6 cancers of the renal pelvis, 2 of the ureter and 15 of the bladder; 4 were infiltrative). Immunohistochemical staining for cyclin E of the analysed transitional cancer cells was assessed semiquantitatively: diffuse cyclin E expression + + + (> 50% of all cells), expression in larger groups of cells: + + (up to 50% of all cells), expression in individual cells or small cell clusters: + (<10% of all cells), and absence of expression. Tumor stage was based on clinical and morphological criteria. WHO classification (Lyon 2004) was used for determination of the histological grade. Results: Non-parametric Spearman's correlation showed that there was no statistically significant correlation between tumor stage and expression of cyclin E(ρ=-0331, p>0.05). Also, no statistically significant correlation between grade and the expression of cyclin E(ρ=-0077, p>0.05) was found. x 2 test results showed no statistically significant difference (x 2 = 2.136, p=0.775) in the expression of cyclin E between upper and lower urothelium. Conclusion: This study showed non significant decreased expression of cyclin E with poor differentiation, muscle invasion and upper/lower urothelium. Expression of cyclin E decreased with increasing histological grade and stage of the tumor. © 2011 Zerbinis Medical Publications.