Browsing by Author "Milasin, J. (6603015594)"

Objectives: This study aimed to analyse Epstein–Barr virus (EBV) and human cytomegalovirus (HCMV) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of TNF-α, IL-1β and IL-6 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines. Materials and Methods: A total of 100 samples of apical periodontitis were subjected to HCMV and EBV copy numbers analysis by nested polymerase chain reaction (PCR) and TaqMan real-time PCR. The concentrations of TNF-α, IL-1β and IL-6 were determined by ELISA method. SPSS software was used for statistical analysis. Results: There were no significant differences in the occurrence of EBV and HCMV between symptomatic and asymptomatic periapical lesions (p =.686, p =.879, respectively). Only 12 of 74 EBV (16.2%) and four of 54 HCMV (13.5%) nested PCR-positive samples showed increased viral copy numbers above the limit of 125 copies/ml. There was no significant correlation between the levels of analysed proinflammatory cytokines and herpesviral copy numbers in our sample. Conclusion: The observed low viral loads point to a relatively rare occurrence of active EBV and HCMV infection in our sample. Latent herpesviral infection does not enhance the production of investigated proinflammatory cytokines. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved

Objectives: This study aimed to analyse Epstein–Barr virus (EBV) and human cytomegalovirus (HCMV) viral loads in symptomatic and asymptomatic apical periodontitis lesions, to determine levels of TNF-α, IL-1β and IL-6 in these lesions and to investigate a possible correlation between herpesviral copy numbers and levels of proinflammatory cytokines. Materials and Methods: A total of 100 samples of apical periodontitis were subjected to HCMV and EBV copy numbers analysis by nested polymerase chain reaction (PCR) and TaqMan real-time PCR. The concentrations of TNF-α, IL-1β and IL-6 were determined by ELISA method. SPSS software was used for statistical analysis. Results: There were no significant differences in the occurrence of EBV and HCMV between symptomatic and asymptomatic periapical lesions (p =.686, p =.879, respectively). Only 12 of 74 EBV (16.2%) and four of 54 HCMV (13.5%) nested PCR-positive samples showed increased viral copy numbers above the limit of 125 copies/ml. There was no significant correlation between the levels of analysed proinflammatory cytokines and herpesviral copy numbers in our sample. Conclusion: The observed low viral loads point to a relatively rare occurrence of active EBV and HCMV infection in our sample. Latent herpesviral infection does not enhance the production of investigated proinflammatory cytokines. © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved

Introduction: Recent studies suggest that some viruses, including human cytomegalovirus (CMV), may be involved in the pathogenesis of periapical lesions. Since periapical cysts (PCs) represent the next stage in the evolution of periapical granuloma, it seemed reasonable to investigate the presence of CMV in PCs and any possible relationship between its presence and the clinical features of those cysts, as well as to compare the results obtained with corresponding findings in non-inflammatory lesions, like odontogenic keratocysts (OKCs). Methods: Samples of 33 PCs and 10 OKCs, obtained at the time of surgery, were used for the detection of CMV DNA by polymerase chain reaction. Presence of the virus was correlated with clinical and radiographic features of the cysts. Results: CMV was detected in 18 PCs (54.5%) and six OKCs (60%). The presence of CMV was more frequent in cyst samples collected from patients who reported previous episodes of acute infection. The presence of sinus tract was more frequent in CMV-positive cysts and CMV presence was less frequent in a group of cysts showing signs of acute inflammation at the time of sample collection. The mean sizes of CMV-positive and CMV-negative PCs were almost the same; CMV-positive OKCs were slightly larger than CMV-negative OKCs. None of these results proved to be statistically significant. Conclusion: The presence of CMV in the cystic wall is a common feature of both inflammatory and non-inflammatory odontogenic cysts. Although this study has not proved that CMV affects pathogenesis of odontogenic cysts, such a possibility could not be ruled out. © 2007 The Authors.

Introduction: Recent studies suggest that some viruses, including human cytomegalovirus (CMV), may be involved in the pathogenesis of periapical lesions. Since periapical cysts (PCs) represent the next stage in the evolution of periapical granuloma, it seemed reasonable to investigate the presence of CMV in PCs and any possible relationship between its presence and the clinical features of those cysts, as well as to compare the results obtained with corresponding findings in non-inflammatory lesions, like odontogenic keratocysts (OKCs). Methods: Samples of 33 PCs and 10 OKCs, obtained at the time of surgery, were used for the detection of CMV DNA by polymerase chain reaction. Presence of the virus was correlated with clinical and radiographic features of the cysts. Results: CMV was detected in 18 PCs (54.5%) and six OKCs (60%). The presence of CMV was more frequent in cyst samples collected from patients who reported previous episodes of acute infection. The presence of sinus tract was more frequent in CMV-positive cysts and CMV presence was less frequent in a group of cysts showing signs of acute inflammation at the time of sample collection. The mean sizes of CMV-positive and CMV-negative PCs were almost the same; CMV-positive OKCs were slightly larger than CMV-negative OKCs. None of these results proved to be statistically significant. Conclusion: The presence of CMV in the cystic wall is a common feature of both inflammatory and non-inflammatory odontogenic cysts. Although this study has not proved that CMV affects pathogenesis of odontogenic cysts, such a possibility could not be ruled out. © 2007 The Authors.

The aim of this study was to detect the loss of putative tumor suppressor genes (TSG) mapping on chromosome 11 in a series of 15 cervical carcinomas. Highly polymorphic microsatellite markers mapping on the selected regions on 11p (11p12 and 11p15.2), and 11q (11q13 and 11q23) were amplified by PCR and used to detect the loss of heterozygosity in our sample. All the tumors were squamous cell carcinomas with various degrees of differentiation. We found that 3 out of 15 cases showed LOH of at least one microsatellite locus on chromosome 11p. LOH on chromosome 11q occurred in 4 samples. All the tumors except one were histologic grade 2, but had heterogeneous clinical stages. The overall incidence of LOH on 11p and 11q in our sample was 18,5% and 29%, respectively. These results might suggest the presence of new tumor suppressor genes (except the well-known WT1) within these chromosomal regions as well as their involvement in cervical carcinogenesis.

The aim of this study was to detect the loss of putative tumor suppressor genes (TSG) mapping on chromosome 11 in a series of 15 cervical carcinomas. Highly polymorphic microsatellite markers mapping on the selected regions on 11p (11p12 and 11p15.2), and 11q (11q13 and 11q23) were amplified by PCR and used to detect the loss of heterozygosity in our sample. All the tumors were squamous cell carcinomas with various degrees of differentiation. We found that 3 out of 15 cases showed LOH of at least one microsatellite locus on chromosome 11p. LOH on chromosome 11q occurred in 4 samples. All the tumors except one were histologic grade 2, but had heterogeneous clinical stages. The overall incidence of LOH on 11p and 11q in our sample was 18,5% and 29%, respectively. These results might suggest the presence of new tumor suppressor genes (except the well-known WT1) within these chromosomal regions as well as their involvement in cervical carcinogenesis.

The primary myelodysplastic syndrome (MDS) is a heterogeneous group of disorders characterized by a failure of the hematopoietic progenitors to differentiate normally, resulting in ineffective and dysplastic hematopoiesis of one or more cell lines. MDS is considered to be preleukemic state and in this context, it provides an opportunity to study molecular mechanisms that precede the development of leukemia. Molecular lesions underlying the evolution of MDS are largely unknown. Mutations of RAS genes have been found in variety of human hematologic malignancies including 5% to 30% of patients with MDS. The ras family of genes encodes 21-Kd guanosine triphosphate (GTP) - binding proteins that play an important role in growth and differentiation signal transduction. Usually, the mutations occur in codon 12, 13 and 61 of the exon I. These three positions are related to the domain of GTP-ase activity and if one of these suffer from mutation, activity of GTP-ase will be affected. The frequency of K-RAS point mutation was studied in 20 pediatric patients with MDs. Archival bone-marrow samples were screened by DNA amplification followed by single-strand conformation polymorphism (SSCP) at codon 12/13 and 61 of K-RAS exon I. No mutations of K-RAS gene were observed in our study suggesting that frequency of K-RAS mutation in childhood primary MDS may be lower than that for adults and that its role in leukemogenesis in primary and secondary MDS is different.

The primary myelodysplastic syndrome (MDS) is a heterogeneous group of disorders characterized by a failure of the hematopoietic progenitors to differentiate normally, resulting in ineffective and dysplastic hematopoiesis of one or more cell lines. MDS is considered to be preleukemic state and in this context, it provides an opportunity to study molecular mechanisms that precede the development of leukemia. Molecular lesions underlying the evolution of MDS are largely unknown. Mutations of RAS genes have been found in variety of human hematologic malignancies including 5% to 30% of patients with MDS. The ras family of genes encodes 21-Kd guanosine triphosphate (GTP) - binding proteins that play an important role in growth and differentiation signal transduction. Usually, the mutations occur in codon 12, 13 and 61 of the exon I. These three positions are related to the domain of GTP-ase activity and if one of these suffer from mutation, activity of GTP-ase will be affected. The frequency of K-RAS point mutation was studied in 20 pediatric patients with MDs. Archival bone-marrow samples were screened by DNA amplification followed by single-strand conformation polymorphism (SSCP) at codon 12/13 and 61 of K-RAS exon I. No mutations of K-RAS gene were observed in our study suggesting that frequency of K-RAS mutation in childhood primary MDS may be lower than that for adults and that its role in leukemogenesis in primary and secondary MDS is different.

Background: The aim of this study was to confirm the presence of herpes simplex virus type 1 and 2 on the oral mucosa, in patients undergoing chemotherapy, by means of polymerase chain reaction (PCR). Methods: The research war carried out on 40 patients receiving chemotherapy as treatment for different malignancies. The status of oral mucosa and viral presence were assessed in all patients at the initial examination (prior to chemotherapy), and at the control examination (two weeks after the initiation of the chemotherapeutic cycle). Results: The presence of HSV-1 was detected in 28 patients (70%) prior to chemotherapy, of whom 7 (25%) manifested oral complications. The control examination showed the presence of HSV-1 in 35 patients (87.5%), of whom 23 (65.7%) presented oral mucosa changes. HSV-2 has not been detected in any of the patients.

Aim: The aim of this study was to determine the prevalence and type of microdeletions of the Y chromosome of men with severe oligozoospermia-ICSI candidates in the Serbian population and to compare our findings with those from other parts of the world. Methods: In all patients spermiogram has been performed in order to determine the sperm concentration. Patients were subjected to detailed clinical, endocrinological and cytogenetic examinations. Microdeletion analysis was performed by polymerase chain reaction (PCR) on 203 patients with normal cytogenetic findings. The STS markers tested in each case were sY84, sY86 (AZFa); SY127, sY134 (AZFb); sY254, sY255 (AZFc). Results: at least one of the STS markers was deleted in 11 of the 203 cases (5.4 %). Conclusion: AZFc microdeletions were identified with a rather high prevalence in men with severe oligozoospermia ICSI candidates in Serbian population.

Purpose: To determine survivin expression patterns in Wilms tumor (WT) and compare it with the expression in normal renal tissue. Also, to analyse cytoplasmic and nuclear survivin expression in relation to histological type, prognostic group and tumor stage. Methods: Immunohistochemical expression of survivin was analysed in 59 cases of primary WT and in 10 normal kidney specimens, taken from the same patients, but distant from the tumor. Results: 51 out of 59 cases of WT (86.44%) showed decreased cytoplasmic survivin expression and 4 out of 59 cases of WT (6.78%) showed nuclear overexpression of survivin. There was statistically significant difference in the frequency of decreased cytoplasmic expression of survivin in individual components of WT (p=0.005). Decreased cytoplasmic expression of survivin in epithelial, blastemal and stromal component was found significantly more often in low stage WT compared to high stage WT (Fisher exact test, p=0.0002, p=0.002, p=0.002, respectively). There was no statistically significant difference in the frequency of survivin nuclear overexpression between different stages of WT (Fisher exact test, p=0.564), histological types (Fisher exact test, p=0.915), or between different prognostic groups (Fisher exact test, p=1). Conclusion: Decreased survivin cytoplasmic expression or nuclear overexpression may be related to favorable prognosis of WT. © 2012 Zerbinis Medical Publications.

Purpose: To determine survivin expression patterns in Wilms tumor (WT) and compare it with the expression in normal renal tissue. Also, to analyse cytoplasmic and nuclear survivin expression in relation to histological type, prognostic group and tumor stage. Methods: Immunohistochemical expression of survivin was analysed in 59 cases of primary WT and in 10 normal kidney specimens, taken from the same patients, but distant from the tumor. Results: 51 out of 59 cases of WT (86.44%) showed decreased cytoplasmic survivin expression and 4 out of 59 cases of WT (6.78%) showed nuclear overexpression of survivin. There was statistically significant difference in the frequency of decreased cytoplasmic expression of survivin in individual components of WT (p=0.005). Decreased cytoplasmic expression of survivin in epithelial, blastemal and stromal component was found significantly more often in low stage WT compared to high stage WT (Fisher exact test, p=0.0002, p=0.002, p=0.002, respectively). There was no statistically significant difference in the frequency of survivin nuclear overexpression between different stages of WT (Fisher exact test, p=0.564), histological types (Fisher exact test, p=0.915), or between different prognostic groups (Fisher exact test, p=1). Conclusion: Decreased survivin cytoplasmic expression or nuclear overexpression may be related to favorable prognosis of WT. © 2012 Zerbinis Medical Publications.

Objectives: The aim of this study was to investigate the expression of survivin, an inhibitor of apoptosis, in odontogenic keratocysts and to compare it to the findings in non-neoplastic jaw cysts - periapical cysts, as well as to establish a possible relationship between survivin expression and human cytomegalovirus presence within these cysts. Materials and methods: Samples of 10 odontogenic keratocysts (five positive and five negative for the presence of cytomegalovirus, as determined by polymerase chain reaction) and 10 periapical cysts (five positive and five negative for the cytomegalovirus presence) were analysed. The expression of survivin was assessed by immunohistochemical methods, using monoclonal antibody that selectively recognizes the cytoplasmic form of survivin. Results: All 10 odontogenic keratocysts showed immunostaining for survivin, while all 10 periapical cysts were negative for its presence. There was no correlation between cytomegalovirus presence and expression of survivin within odontogenic keratocysts. Conclusion: Survivin may contribute to the aggressive behavior of odontogenic keratocysts, and thus support the emerging opinion of their neoplastic nature. © 2009 John Wiley & Sons A/S.

Objectives: The aim of this study was to investigate the expression of survivin, an inhibitor of apoptosis, in odontogenic keratocysts and to compare it to the findings in non-neoplastic jaw cysts - periapical cysts, as well as to establish a possible relationship between survivin expression and human cytomegalovirus presence within these cysts. Materials and methods: Samples of 10 odontogenic keratocysts (five positive and five negative for the presence of cytomegalovirus, as determined by polymerase chain reaction) and 10 periapical cysts (five positive and five negative for the cytomegalovirus presence) were analysed. The expression of survivin was assessed by immunohistochemical methods, using monoclonal antibody that selectively recognizes the cytoplasmic form of survivin. Results: All 10 odontogenic keratocysts showed immunostaining for survivin, while all 10 periapical cysts were negative for its presence. There was no correlation between cytomegalovirus presence and expression of survivin within odontogenic keratocysts. Conclusion: Survivin may contribute to the aggressive behavior of odontogenic keratocysts, and thus support the emerging opinion of their neoplastic nature. © 2009 John Wiley & Sons A/S.

Liveborn infants with tetraploidy are very rare in human pregnancies and usually die during the first days or months. Seven cases of liveborn infants with tetraploidy have previously been reported. Among them only two 92, XXXX infants survived for longer than 12 months. Here we report on the case of a 26-month-old girl with tetraploidy. The main clinical features of tetraploidy are facial dysmorphism, severely delayed growth and developmental delay. On the basis of molecular studies we discuss the possible origin of the additional chromosome sets in our proband. To our knowledge, this infant is the first reported case of tetraploidy who lived up to 26 months.

Liveborn infants with tetraploidy are very rare in human pregnancies and usually die during the first days or months. Seven cases of liveborn infants with tetraploidy have previously been reported. Among them only two 92, XXXX infants survived for longer than 12 months. Here we report on the case of a 26-month-old girl with tetraploidy. The main clinical features of tetraploidy are facial dysmorphism, severely delayed growth and developmental delay. On the basis of molecular studies we discuss the possible origin of the additional chromosome sets in our proband. To our knowledge, this infant is the first reported case of tetraploidy who lived up to 26 months.