Browsing by Author "Mikic, Anton (22941219500)"

The purpose of the present study was to examine the expression of CD105 among patients with supraglottic laryngeal cancer and to assess the clinical relevance of CD105-assessed MVD. A total of 40 patients with supraglottic squamous cell carcinomas (SCCs) were included in the present study. Surgical specimens were immunostained for CD105 and MVD was calculated at 400× magnification. The rounded mean value of the vessel count in four fields for each case was used as the final MVD value. The mean MVD value assessed by CD105 in considered supraglottic SCCs was 13.5 (SD = 3.97). High MVD was significantly correlated with advanced (III and IV) clinical stage (Mann-Whitney U test P = 0.01) and malignancy recurrence presence/absence (Mann-Whitney U test P = 0.023). Spearman's rank correlation test showed significant correlation between high CD105-assessed MVD and pN+ category (rho = 0.337, P = 0.033), advanced Stage (III and IV) (rho = 0.402, P = 0.01) and developed locoregional recurrence (rho = 0.395, P = 0.012). The logistic regression showed that a high CD105+ MVD was the only independent marker of tumor recurrence (P = 0.029; odds ratio, 6.64; 95% CI, 1.218-36.152). The average MVD was significantly higher in patients with advanced TNM stage and in patients with locoregional recurrence of disease, suggesting that angiogenesis is closely related with clinical aggressiveness of tumor. CD105-assessed MVD in supraglottic laryngeal SSCs may identify patients at risk of recurrence of disease. © 2009 Springer-Verlag.

Objective: Intratumoral microvessel density (MVD) determined with the use of antibodies to endoglin (CD105) is considered to be an important prognostic marker in a variety of malignancies. The purpose of this study has been to analyze the clinicopathologic significance of CD105-assessed MVD in SCCs primary localized in glottic region of larynx. Methods: Surgical specimens from 40 patients with resected glottic squamous cell carcinomas were immunostained for CD105. CD105-assessed MVD was calculated at 400× magnification. Using the mean MVD as a cut-off, tumors were classified in the "high MVD" group and in the "low MVD" group. Clinicopathologic data were collected retrospectively. Results: The mean MVD assessed by CD105 in considered glottic SCCs was 12.3 (standard deviation [SD] = 3.65). MVD varied among tissue samples from 5 to 21 (median 12.5). High MVD was significantly correlated with a more aggressive tumor phenotype, including T3-T4 tumor (Fisher exact test, P = 0.004) and advanced clinical stage (Fisher exact test P = 0.026). Kruskal-Wallis test identified significant relation between pT stages and CD105-assessed MVD (P = 0.011). CD105-assessed MVD was significantly related to malignancy recurrence presence/absence (Mann-Whitney U-test P = 0.023). Logistic regression in multivariate modality showed that MVD (odds ratio [OR] 2.29, P = 0.033, 95% confidence interval [CI] 1.06-7.53) and advanced T category (T3-T4) (OR 4.11, P = 0.026, 95% CI 2.38-9.46) were significantly related to malignancy recurrence presence/absence. Cox regression analysis revealed that expression of CD105 (P = 0.031) and N status (P = 0.014) were the independent factors for disease-free survival. Conclusion: High expression of CD105 correlated significantly with advanced T status and locoregional recurrence. The present preliminary results suggest that CD105-assessed MVD in primary glottic squamous cell carcinomas may identify patients at risk of disease recurrence. © 2009 Elsevier Ireland Ltd. All rights reserved.

Background We provided preliminary psychometric data for the European Organization for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire-Head and Neck Module, updated version (QLQ-H&N43) from a group of Serbian laryngectomized patients. Methods The study included 170 subjects. The QLQ-H&N43 is a 43-item questionnaire, with 12 multi-item scales and 7 single-item symptom scales. All subjects also completed the Quality of Life Questionnaire-Core 30-questions (QLQ-C30). Results Good internal consistency (Cronbach's α of above 0.7) was found for 5 of the 7 scales. All QLQ-H&N43 scales correlated negatively as predicted with all QLQ-C30 functioning scales. The correlations with the QLQ-C30 symptoms supported discriminant validity, with only one exception: the head and neck social eating scale overlapped with the QLQ-C30 pain scale. For 14 of 19 QLQ-H&N43 scale scores, significant known-group differences were observed between those who differ in type of laryngectomy, adjuvant therapy, or 5-year survival. Conclusion Preliminary evidence suggests that a great majority of the QLQ-H&N43 scales have acceptable internal consistency and promising construct validity, but more research studies are needed with other cancer groups to extend these findings. © 2015 Wiley Periodicals, Inc..

Objective: To examine immunohistochemical expression of CD105 among patients with laryngeal cancer and investigate the prognostic significance of CD105-assessed microvessel density (MVD). Study Design: Cross-sectional study. Setting: University hospital. Subjects and Methods: The study comprised 80 patients with laryngeal squamous cell carcinoma who underwent complete excision. Clinicopathological data were collected retrospectively. Immunohistochemical analysis was performed with CD105 (endoglin) antibody. Positive-stained microvessels for CD105 were counted on hot spots of tumors at ×200 magnification. Results: Average CD105-assessed MVD in considered laryngeal squamous cell carcinomas (SCCs) was 12.9 (SD 3.84). High expression of CD105 correlated significantly with advanced T (tumor) classification (P = 0.008), advanced TNM (tumor, node, metastasis) stage (P = 0.001), tumor recurrence (P = 0.001), and age ≥65 years (P = 0.026). The multivariate logistic regression showed that a high CD105+ MVD (odds ratio [OR] 4.27; P = 0.019) and advanced TNM stage (OR 3.72; P = 0.047) were independent markers of tumor recurrence. High MVD, advanced clinical stage, the presence of lymph node metastasis at the time of diagnosis, and age <66 years were associated with worse disease-free survival. Cox regression analysis revealed that expression of CD105 (P = 0.016) and advanced clinical stage (P = 0.05) were the independent factors for disease-free survival. Conclusion: The present results suggest that MVD evaluation with CD105 is a promising prognostic factor for the outcome of patients with laryngeal SCC. CD105-assessed MVD could help to identify patients with more aggressive disease and increased risk of developing malignancy recurrence after treatment. © 2009 American Academy of Otolaryngology-Head and Neck Surgery Foundation.

There has recently been one hundred years since George Crile demonstrated systematic cervical lymph nodes excision for treatment of patients with head and neck cancer. Due to morbidity of the radical surgical approach, the emphasis was made on surgically conservative approach, in cases where it does not have adverse effects on disease control and still offers preservation of functional and cosmetic status. Modified radical neck dissection (MRND) removes "en block" the same lymph nodes and vessels as radical dissection, but by preserving one or more non-lymphatic structures that are being entirely removed by radical dissection, it decreases postoperative morbidity. Selective neck dissections, which are being preferred by American head and neck surgeons, went even further. The research was performed at Institute for Otolaryngology and Maxillofacial Surgery of Clinical Center of Serbia in Belgrade. The study included 319 patients treated period, from January 1, 1997 to December 31, 2001, who have been followed up for at least 5 years after treatment. Having in mind that this was retrospective clinical study, i.e. longitudinal study, the choice of patients and variables was made based on patient records. Of 302 patients with determined category, the most were in T2 category (40.44%), while T1 tumors were four times less frequent. It has been observed that metastases recurrences have occurred in 57/319 (17.86%) subjects at various time intervals. By analyzing recurrent metastases according to N categories, it has been determined that the most of them were in N1 and N2 category. This was expected, as N1 category was the most represented in the entire group of patients with 137/319 (42.94%) cases, and the largest number of neck dissections - 137/291 (47.08%), primarily SND, were performed within this category. Patient five-year survival in our study has been analyzed according to N category, type of neck dissection, and recurrence of metastases (Figure 2). Over the first two years of follow-up, there has been larger survival tendency in MRND group compared to SND. The difference became comparable in the third year, and increased in favor of SND by the end of follow-up period. Although arithmetic mean value was almost the same, and median value was higher in patients with MRND, there is no significant difference in five-year survival among patients with N1 category (0.43; df=1; p>0.05).

Background: E-cadherin is a 120 kDa transmembrane protein that is thought to play an important role in malignant progression of tumours and in tumour differentiation. A reduced or absent expression of E-cadherin has been observed in several carcinomas, including squamous cell carcinoma of the head and neck. Objective: The aim of this study was to analyse the clinicopathologic significance of E-cadherin expression in squamous cell carcinomas with a primary location in the glottic region of the larynx. Materials and methods: E-cadherin expression was determined by immunohistochemistry in paraffin-embedded tissue specimens from 40 patients with squamous cell carcinoma of the glottic larynx. A staining score was given based on the percentage of cells stained (0-100%). All stained cells were considered positive regardless of the intensity of the staining. Using the mean expression of Ecadherin as a cut-off, 17 (42.5%) tumours were classified into the "high E-cadherin" group and 23 (57.5%) into the "low E-cadherin" group. Results: E-cadherin expression varied greatly among the tissue samples, with scores ranging from 2 to 72 (median 23). The mean expression score for E-cadherin was 27.35 (standard deviation [SD]=20.15). Decreased E-cadherin expression was significantly correlated with more aggressive tumours, including tumours staged as T3 or T4 (p = 0.038) and those with advanced clinical stage (TNM stage III and IV) (p = 0.010). The results of a stepwise logistic regression analysis showed that only the presence of lymph node metastasis was an independent predictor for tumour recurrence (p=0.019). A Cox proportional hazards model confirmed that the presence of cervical lymph node metastases (P=0.003) and age ≤ 59 years (P=0.006) were statistically significant independent predictors of a reduced disease-specific survival. Conclusion: Expression of E-cadherin may be useful to identify patients with aggressive disease, allowing more effective treatment strategies to be implemented. Larger studies are required to confirm the role of E-cadherin expression in predicting the behaviour of laryngeal squamous cell carcinomas.