Browsing by Author "Laketić, Darko (25936376800)"

Introduction/Objective This research aims to evaluate the impact of chronic stress on behavioral effects of ketamine, which are still not sufficiently clear. Methods Wistar male rats aged five weeks were used in the experiment. The animals were divided into two equal groups: control and experimental. After being exposed to a chronic unpredictable stress paradigm for 42 days, experimental rats received a single injection of ketamine (10 mg/kg; day 45) as did the control group. The impact of ketamine was assessed using behavioral tests, spontaneous coordinate activity, and water maze tests for the evaluation of short-term memory. Results The experimental group rats showed less spontaneous motoric activity than before ketamine application. Statistical significance was shown in gaining weight after time of ketamine application in the control group, as well as in the experimental group, where they showed weight loss during stress paradigm and then increased their weight after ketamine application. There was no statistical significance in speed measurements in either group, showing no effects on short-term memory behavior. Conclusion These findings show that ketamine in a single subanesthetic dose has antidepressant and anxiolytic-like effects in male rats exposed to chronic unpredictable stress paradigm. © 2021, Serbia Medical Society. All rights reserved.

Introduction/Objective The aim of this study was to examine the immunohistochemical features of the vascularization of the anterior cruciate ligament (ACL), as well as the quantification of capillaries within the three segments of the ACL; proximal, middle and distal. The quantification and metric characteristics of mast cells of the ACL are the second goal of this research. Methods In total, 30 human ACL of 30 persons, obtained during routine autopsy, were examined under the microscope, following immunohistochemical reactions against CD34 of blood vessels and MastTrip of mast cells. Results The middle genicular artery close to the ACL gave off branches for the supply of ligament itself. Each field of mm2 contained an average number of 1113.84 (959–1240), microvessels in ACL proximal third, an average number of 1145.43 (924–1310) microvessels in ACL middle third, and an average number of 1134.55 (889–1451) microvessels in ACL distal third. An average number of mast cells of the ACL was 3.8 per mm2. In the peripheral synovial zone of the ACL, we counted 12.6 mast cells per mm2. An average area value of the mast cells was 124.7 μm2, and an average value of shorter and longer axis of the mast cells was 11.2 × 15.0 μm. Conclusion There was no statistically significant differences between the average numbers of intraliga-mentous microvessels of the ACL thirds (p > 0.05), confirming and supporting our hypothesis of uniform distribution of blood supply within the ACL. © 2022, Serbia Medical Society. All rights reserved.

Introduction/Objective Rib fractures are common in blunt chest trauma (BCT), and when they are associated with pleural complications (PC) – pneumothorax, hemothorax and hemopneumothorax – the treatment of these patients is prolonged and difficult. Without the ability to predict PC after rib fractures in BCT, most doctors are forced to initially treat these patients through observation and conservative treatment. The goal of this research is to determine which of the investigated biomarkers of inflammation – fibrino-gen, interleukin-6 (IL-6), and C-reactive protein (CRP) – are significantly associated with the occurrence of PC after rib fracture in BCT, and whether they can be used in stratifying patients for hospitalization and further treatment. Methods The prospective study included 90 patients with rib fractures caused by BCT. The test group comprised 45 patients with rib fractures and the presence of PC, and the control group consisted of 45 patients with rib fractures without PC. Blood sampling was performed on admission, on the second, third, and fifth day after the injury, and PC were monitored until the seventh day after the injury. Results Serum values of IL-6 on the second day and fibrinogen and CRP on the second and third day after injury were statistically significantly higher in patients with PC, and IL-6 showed a good discriminative ability in assessing the occurrence of PC on the second day after a rib fracture in BCT. Conclusion The investigated biomarkers of inflammation – fibrinogen, IL-6, and CRP – can be used as predictors of PC after rib fracture in BCT, and their application can significantly replace clinical observation. © 2023, Serbia Medical Society. All rights reserved.