Browsing by Author "Jelovac, Drago (58449716800)"

Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associatedmarkers,includingvimentin,α-smoothmuscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics. © 2020 Spandidos Publications. All rights reserved.

Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associatedmarkers,includingvimentin,α-smoothmuscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were also higher in the fifth passage compared with those noted at the first with a few exceptions. The rates of proliferation and cell migration were decreased during passages, while the number of colonies was increased in both types of cell culture. Tumor and margin cells indicated certain similarities with regard to EMT transition characteristics. © 2020 Spandidos Publications. All rights reserved.

Background/Aim. Although radical surgical treatment is the method of choice in the therapy of cancer of the maxilla and maxillary sinus, it can cause oral dysfunction, social isolation, and emotional and psychological distress, which significantly affects the patient’s quality of life (QoL). The aim of the study was to determine the health-related QoL of patients rehabilitated with obturator prosthesis (OP) after maxillectomy, according to demographic and clinical characteristics. Methods. The study included 32 patients with a mean age of 63.6 years. The measurement of QoL of patients after maxillectomy and prosthetic rehabilitation was assessed using the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 (EORTC QLQ-C30) and EORTC QLQ – Head and Neck Cancer Module (QLQ-H&N43). Results. Functioning and symptoms were more pronounced in women, younger patients, and single patients (p < 0.05). Patients after definitive prosthetics rehabilitation had fewer problems and symptoms compared to patients with surgical and interim OP (p < 0.05). Patients with partial obturator dentures had a better perception of function than patients with total dentures (p < 0.05). Irradiated patients had more pronounced fatigue, appetite loss, pain in the mouth, dry mouth and sticky saliva, and sense and skin problems compared to nonirradiated patients (p < 0.05). Conclusion. The results of this study suggest that sex, age, marital status, characteristics of OP, and radiotherapy have a significant impact on QoL in patients after maxillectomy. © 2023 Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.

Background: Polymorphisms in genes encoding tumor necrosis factor-α (TNF-α) and its receptor TNF-R1 have been shown to affect one person's susceptibility to develop certain neoplastic diseases. The aim of the present association study was to investigate whether single nucleotide polymorphisms (SNPs) in TNF-α (−308G>A) and TNF-R1 (36A>G) genes modulate the susceptibility for keratocystic odontogenic tumors (KCOTs) development in Serbian patients. Methods: Genotyping was performed in 60 KCOT patients and 125 healthy individuals, using polymerase chain reaction/restriction fragment length polymorphism analysis. Results: A significant difference in genotype and allele frequencies was found between patients and controls for both SNPs (P < 0.05). Carriers of the TNF-α A variant had an eightfold increase of KCOT risk (OR = 8.12, 95% CI = 3.98–16.56, P < 0.0001), while carriers of the TNF-R1 G variant had approximately a fourfold increase of KCOT risk (OR=3.65, CI: 1.60–8.40, P = 0.001). Conclusions: Our findings suggest that the two polymorphisms are strong risk factors for KCOT development in Serbian population. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Background: Polymorphisms in genes encoding tumor necrosis factor-α (TNF-α) and its receptor TNF-R1 have been shown to affect one person's susceptibility to develop certain neoplastic diseases. The aim of the present association study was to investigate whether single nucleotide polymorphisms (SNPs) in TNF-α (−308G>A) and TNF-R1 (36A>G) genes modulate the susceptibility for keratocystic odontogenic tumors (KCOTs) development in Serbian patients. Methods: Genotyping was performed in 60 KCOT patients and 125 healthy individuals, using polymerase chain reaction/restriction fragment length polymorphism analysis. Results: A significant difference in genotype and allele frequencies was found between patients and controls for both SNPs (P < 0.05). Carriers of the TNF-α A variant had an eightfold increase of KCOT risk (OR = 8.12, 95% CI = 3.98–16.56, P < 0.0001), while carriers of the TNF-R1 G variant had approximately a fourfold increase of KCOT risk (OR=3.65, CI: 1.60–8.40, P = 0.001). Conclusions: Our findings suggest that the two polymorphisms are strong risk factors for KCOT development in Serbian population. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd