Browsing by Author "Gotić, M. (7004685432)"

Introduction: Philadelphia-negative myeloproliferative neoplasms (Ph−MPN) are characterized by overproduction of one or more blood cell lines. Methods: We studied the proliferative characteristics of 91 patients with de novo Ph−MPN. Colony-forming cells (CFC) and endogenous colonies (EC), from bone marrow (BM) and/or peripheral blood (PB), were analyzed by colony assay based on methylcellulose. The level of circulating CD34+ cells was determined by flow cytometry. Results: The total number of PB CFC in primary myelofibrosis (PMF) was increased compared to the control sample (P < 0.01) and essential thrombocythemia (ET) (P < 0.05). The highest number of BM and PB EC was observed in polycythemia vera (PV) (P < 0.01). Increased levels of CD34+ cells characterized early-prefibrotic (57%) and advanced-fibrotic PMF (90%) as compared to PV (34%) and ET (32%) (P < 0.01). In the whole Ph−MPN group, the total number of PB CFC (P < 0.01), PB EC (P < 0.05), and CD34+ cells (P < 0.01) correlated with the degree of BM fibrosis. Higher levels of circulating CD34+ cells in PMF correlated with the total number of PB EC (P < 0.05) and degree of BM fibrosis (P < 0.01). Conclusions: Exploration of the PB proliferative characteristics of Ph−MPN on diagnosis may be helpful in revealing early-prefibrotic PMF. Monitoring the levels of circulating CD34+ cells may provide a sensitive indicator of fibrotic evolution in PV and PMF. © 2016 John Wiley & Sons Ltd

Introduction: Philadelphia-negative myeloproliferative neoplasms (Ph−MPN) are characterized by overproduction of one or more blood cell lines. Methods: We studied the proliferative characteristics of 91 patients with de novo Ph−MPN. Colony-forming cells (CFC) and endogenous colonies (EC), from bone marrow (BM) and/or peripheral blood (PB), were analyzed by colony assay based on methylcellulose. The level of circulating CD34+ cells was determined by flow cytometry. Results: The total number of PB CFC in primary myelofibrosis (PMF) was increased compared to the control sample (P < 0.01) and essential thrombocythemia (ET) (P < 0.05). The highest number of BM and PB EC was observed in polycythemia vera (PV) (P < 0.01). Increased levels of CD34+ cells characterized early-prefibrotic (57%) and advanced-fibrotic PMF (90%) as compared to PV (34%) and ET (32%) (P < 0.01). In the whole Ph−MPN group, the total number of PB CFC (P < 0.01), PB EC (P < 0.05), and CD34+ cells (P < 0.01) correlated with the degree of BM fibrosis. Higher levels of circulating CD34+ cells in PMF correlated with the total number of PB EC (P < 0.05) and degree of BM fibrosis (P < 0.01). Conclusions: Exploration of the PB proliferative characteristics of Ph−MPN on diagnosis may be helpful in revealing early-prefibrotic PMF. Monitoring the levels of circulating CD34+ cells may provide a sensitive indicator of fibrotic evolution in PV and PMF. © 2016 John Wiley & Sons Ltd

Abstract: An unusual triclonal IgG combination in the serum of a 56‐year old male with clinical stage IIIB multiple myeloma is reported. The patient initially had an IgG4(Λ) monoclonal protein in his serum and later developed an IgG2(κ) and an IgG (κ) which possessed the characteristics of both IgG1 and IgG3 subclasses with an unusual combination of allotypic markers. Three M‐proteins did not share idiotypic determinants. A rare class‐switch recombination followed by mutation has been considered as a possible mechanism leading to this combination. © Munksgaard 1995