Browsing by Author "Dunjic, Marija (23472894200)"

Introduction: HPV is a family of double-stranded DNA viruses that infect the mucous epithelium. Most of these infections regress without consequences; however, the high-risk HPV viral strains can determine a tumor transformation of the epithelium, giving rise to tumor forms such as cervical cancer. The analysis mostly applied to define abnormalities of the membranes is the PAP test and the treatment is reconciled, in most cases through prevention. Alongside the standard protocol, we have determined that a blend of essential oils has an effect in determining the negativization of HPV. Materials and Methods: We made use of the BDORT technique in order to determine the specific percentages of each component of the prepared oil blend. To confirm this, two advanced Molecular Docking software have been applied (Swiss Dock and 1-Click Docking). The mixture of oils was subsequently applied, in the form of vaginal capsules, on a group of HPV (+) positive patients, who were tested for the presence of a virus, before and after treatment, by RT-PCR, Colposcopy, and BDORT to evaluate the state of epithelium before and after the assumption of oil vaginal capsules. Results: The mixture of oils has produced the negativization of HPV, determined by BDORT and confirmed by RT-PCR, in the treated patients. Discussion: We have determined that the blend of essential oils, prepared here, influenced the negativization of the virus, in all the patients treated. Conclusions: The operative efficacy of these oils in the negativization of HPV has been demonstrated. Copyright © 2023 Cognizant Communication Corp.

Introduction: HPV is a family of double-stranded DNA viruses that infect the mucous epithelium. Most of these infections regress without consequences; however, the high-risk HPV viral strains can determine a tumor transformation of the epithelium, giving rise to tumor forms such as cervical cancer. The analysis mostly applied to define abnormalities of the membranes is the PAP test and the treatment is reconciled, in most cases through prevention. Alongside the standard protocol, we have determined that a blend of essential oils has an effect in determining the negativization of HPV. Materials and Methods: We made use of the BDORT technique in order to determine the specific percentages of each component of the prepared oil blend. To confirm this, two advanced Molecular Docking software have been applied (Swiss Dock and 1-Click Docking). The mixture of oils was subsequently applied, in the form of vaginal capsules, on a group of HPV (+) positive patients, who were tested for the presence of a virus, before and after treatment, by RT-PCR, Colposcopy, and BDORT to evaluate the state of epithelium before and after the assumption of oil vaginal capsules. Results: The mixture of oils has produced the negativization of HPV, determined by BDORT and confirmed by RT-PCR, in the treated patients. Discussion: We have determined that the blend of essential oils, prepared here, influenced the negativization of the virus, in all the patients treated. Conclusions: The operative efficacy of these oils in the negativization of HPV has been demonstrated. Copyright © 2023 Cognizant Communication Corp.

Radiofrequency therapy is an unconventional method, already applied for some time, with numerous results in numerous clinical pictures. Our group has developed a software, later called SONGENPROT-SOLARIS, capable of directly converting nucleotide sequences (DNA and/or RNA) and amino acid sequences (polypeptides and proteins) into musical sequences, based on mathematic matrices, designed by the French physicist and musician Joel Sternheimer, which allows to associate a musical note with a nucleotide or an amino acid. Innovation in our software is that, in the algorithm that defines it, a variant is directly implemented that allows the reproduction of sounds, phase-shifted by 30 Hz, between one ear and another reproducing the phenomenon of Binaural Tones, capable of induce a specific brain activity and also the release of particles called solitons. Thanks to this software we have developed a technique called MMT (Molecular Music Therapy) and currently, we are in the phase of applying the technique on a cohort of 91 patients, with a high spectrum of clinical pictures, examining the same, using the technique Bi-Digital-ORing- Test (BDORT), before and after treatment with MMT. Aim of project is to stimulate the expression of a specific gene (the same genetic sequence that the patient listens to, translated into music), only through the use of sound sequences. We have concentrated our attention on three main molecules: Sirtuin-1, Telomers and TP-53. The results obtained with BDORT, after treatment with MMT, showed a significant increase in the values of the three molecules, on all the examined patients, demonstrating the operative efficacy of the technique and the its applicability to numerous diseases. In order to confirm the data obtained by BDORT, we propose, with the help of an accredited laboratory, to perform epigenetic tests on the three parameters listed above, paving the way to understanding how frequencies can influence gene expression. Copyright © 2020 Cognizant Communication Corp.

Radiofrequency therapy is an unconventional method, already applied for some time, with numerous results in numerous clinical pictures. Our group has developed a software, later called SONGENPROT-SOLARIS, capable of directly converting nucleotide sequences (DNA and/or RNA) and amino acid sequences (polypeptides and proteins) into musical sequences, based on mathematic matrices, designed by the French physicist and musician Joel Sternheimer, which allows to associate a musical note with a nucleotide or an amino acid. Innovation in our software is that, in the algorithm that defines it, a variant is directly implemented that allows the reproduction of sounds, phase-shifted by 30 Hz, between one ear and another reproducing the phenomenon of Binaural Tones, capable of induce a specific brain activity and also the release of particles called solitons. Thanks to this software we have developed a technique called MMT (Molecular Music Therapy) and currently, we are in the phase of applying the technique on a cohort of 91 patients, with a high spectrum of clinical pictures, examining the same, using the technique Bi-Digital-ORing- Test (BDORT), before and after treatment with MMT. Aim of project is to stimulate the expression of a specific gene (the same genetic sequence that the patient listens to, translated into music), only through the use of sound sequences. We have concentrated our attention on three main molecules: Sirtuin-1, Telomers and TP-53. The results obtained with BDORT, after treatment with MMT, showed a significant increase in the values of the three molecules, on all the examined patients, demonstrating the operative efficacy of the technique and the its applicability to numerous diseases. In order to confirm the data obtained by BDORT, we propose, with the help of an accredited laboratory, to perform epigenetic tests on the three parameters listed above, paving the way to understanding how frequencies can influence gene expression. Copyright © 2020 Cognizant Communication Corp.

This study presents a comparative analysis of molecular docking data, focusing on the binding interactions of the natural compounds apigenin and luteolin with the proteins TP-53, pRb, and APOBEC, in comparison to conventional pharmacological ligands. Advanced bioinformatics techniques were employed to evaluate and contrast binding energies, showing that apigenin and luteolin demonstrate significantly higher affinities for TP-53, pRb, and APOBEC, with binding energies of −6.9 kcal/mol and −6.6 kcal/mol, respectively. These values suggest strong potential for therapeutic intervention against HPV-16. Conventional ligands, by comparison, exhibited lower affinities, with energies ranging from −4.5 to −5.5 kcal/mol. Additionally, protein–protein docking simulations were performed to assess the interaction between HPV-16 E6 oncoprotein and tumor suppressors TP-53 and pRb, which revealed high binding energies around −976.7 kcal/mol, indicative of their complex interaction. A conversion formula was applied to translate these protein–protein interaction energies to a comparable scale for non-protein interactions, further underscoring the superior binding potential of apigenin and luteolin. These findings highlight the therapeutic promise of these natural compounds in preventing HPV-16-induced oncogenesis, warranting further experimental validation for clinical applications. © 2024 by the authors.

This study presents a comparative analysis of molecular docking data, focusing on the binding interactions of the natural compounds apigenin and luteolin with the proteins TP-53, pRb, and APOBEC, in comparison to conventional pharmacological ligands. Advanced bioinformatics techniques were employed to evaluate and contrast binding energies, showing that apigenin and luteolin demonstrate significantly higher affinities for TP-53, pRb, and APOBEC, with binding energies of −6.9 kcal/mol and −6.6 kcal/mol, respectively. These values suggest strong potential for therapeutic intervention against HPV-16. Conventional ligands, by comparison, exhibited lower affinities, with energies ranging from −4.5 to −5.5 kcal/mol. Additionally, protein–protein docking simulations were performed to assess the interaction between HPV-16 E6 oncoprotein and tumor suppressors TP-53 and pRb, which revealed high binding energies around −976.7 kcal/mol, indicative of their complex interaction. A conversion formula was applied to translate these protein–protein interaction energies to a comparable scale for non-protein interactions, further underscoring the superior binding potential of apigenin and luteolin. These findings highlight the therapeutic promise of these natural compounds in preventing HPV-16-induced oncogenesis, warranting further experimental validation for clinical applications. © 2024 by the authors.

The aim of the research is to determine whether there was synergism between mobile phone exposure and higher release and accumulation of mercury in tissues of the individuals with amalgam fillings. For this purpose, the computer electro-magnetic simulation and the Bi-Digital O-Ring Test (BDORT) method were used. For the electro-magnetic simulation, the FIT method (Finite Integration technique - numerical simulation method for calculation elelctromagnentic fields in space, expecialy in body) was used and the results of the electric field and Specific absorption rate (SAR) in the tooth model with and without amalgam fillings were obtained. Specific absorption rate is a measure of the rate at which energy is absorbed by the human body when exposed to a radio frequency (RF) electromagnetic field. It is dose for absorbed electromagnetic radiation which is dependent on frequency and can be associated with the adverse effect of tissue heating. Standards indicate SAR values that should not be exceeded. The numerical result of absorption electro-magnetic energy shows increased value of the electric field, magnetic field, induced currents and SAR in teeth with fillings. These results indicated the possible explanation of the increased release mechanism of the mercury (Hg) when using mobile phones. The Bi-Digital O-Ring Test method tested the levels of mercury in the body, liver, and urine in a group of examinees who had amalgam fillings and used cell phones. By using indirect BDORT we examined groups of examinees 10 with and 10 examinees without amalgam fillings. By using BDORT we measaured level ofHg in the body and urin samples in all examinees before exposure to electromagnetic fields (EMFs) of mobile phone and after 15 minutes of exposure of mobile phone EMF. The results showed significantly higher level of Hg in urin after exposure of EMF of mobile phone, only in a group of examinees with amalgam fillings. The results showed significant matches with some published papers by other researchers. These effects might cause potential organism disorders and represent secondary harmful effects of electro-magnetic radiation on the health of individuals. This approach throws a new light in clarifying the interplay of cellular electro-magnetic radiation and increased the release of mercury from the amalgam fillings. Increased toxic effects are imposed by themselves as possible. Copyright © 2019 Cognizant Communication Corp.

The aim of the research is to determine whether there was synergism between mobile phone exposure and higher release and accumulation of mercury in tissues of the individuals with amalgam fillings. For this purpose, the computer electro-magnetic simulation and the Bi-Digital O-Ring Test (BDORT) method were used. For the electro-magnetic simulation, the FIT method (Finite Integration technique - numerical simulation method for calculation elelctromagnentic fields in space, expecialy in body) was used and the results of the electric field and Specific absorption rate (SAR) in the tooth model with and without amalgam fillings were obtained. Specific absorption rate is a measure of the rate at which energy is absorbed by the human body when exposed to a radio frequency (RF) electromagnetic field. It is dose for absorbed electromagnetic radiation which is dependent on frequency and can be associated with the adverse effect of tissue heating. Standards indicate SAR values that should not be exceeded. The numerical result of absorption electro-magnetic energy shows increased value of the electric field, magnetic field, induced currents and SAR in teeth with fillings. These results indicated the possible explanation of the increased release mechanism of the mercury (Hg) when using mobile phones. The Bi-Digital O-Ring Test method tested the levels of mercury in the body, liver, and urine in a group of examinees who had amalgam fillings and used cell phones. By using indirect BDORT we examined groups of examinees 10 with and 10 examinees without amalgam fillings. By using BDORT we measaured level ofHg in the body and urin samples in all examinees before exposure to electromagnetic fields (EMFs) of mobile phone and after 15 minutes of exposure of mobile phone EMF. The results showed significantly higher level of Hg in urin after exposure of EMF of mobile phone, only in a group of examinees with amalgam fillings. The results showed significant matches with some published papers by other researchers. These effects might cause potential organism disorders and represent secondary harmful effects of electro-magnetic radiation on the health of individuals. This approach throws a new light in clarifying the interplay of cellular electro-magnetic radiation and increased the release of mercury from the amalgam fillings. Increased toxic effects are imposed by themselves as possible. Copyright © 2019 Cognizant Communication Corp.

Human papillomavirus (HPV) is a virus from the papillomavirus family that is capable of infecting humans. Some types of HPVs cause warts, while others can lead to cancers of the cervix, vulva, vagina, penis, oropharynx and anus. Highrisk human papillomavirus (hr HPV) has been detected in almost all cervical squamous cell carcinomas and adenocarcinomas. All patients examined by colposcopy. Cervical swab is routinely done and patients are screened with both HPV DNA by Real Time Polimerase Chain Reaction (RT PCR) testing and Pap testing. Pictures obtained by colposcopy were examined by indirect Bi-Digital O-Ring Test (BDORT) by using reference control substance (RCS): HPV 16, HPV 18, and Integrin α5 βl. BDORT was developed by Prof. Omura Y. of New York and received U.S. patent in 1993. For detection of HPV DNA we used RT PCR and standard Qiagen method which detect 18 types (16,18,31,33,35,39,45,51,52,56,58,59,68, 6,11,42,43,44) of HPV from smear. From 63 patients where is BDORT indicated presence of HPV, in 49 pateints (77,8%) RT PCR confirmed presence of HPV. From 63 patients in 54 patients (85,7%), we detected, by colposcopic exam, some kind of lesions associated with HPV infection. Results obtained by RT PCR: one type (1/18) of DNA HPV in 25. patients (51,02%), 2 types (2/18) in 15 patients (30,61%) and 3 types (3/18) in 9 patients (18,37%). Although BDORT results usually have higher sensitivity and detection rate is much higher, it can be used together with RT PCR in detection of HPV and cervical lesions associated with HPV infection.

Human papillomavirus (HPV) is a virus from the papillomavirus family that is capable of infecting humans. Some types of HPVs cause warts, while others can lead to cancers of the cervix, vulva, vagina, penis, oropharynx and anus. Highrisk human papillomavirus (hr HPV) has been detected in almost all cervical squamous cell carcinomas and adenocarcinomas. All patients examined by colposcopy. Cervical swab is routinely done and patients are screened with both HPV DNA by Real Time Polimerase Chain Reaction (RT PCR) testing and Pap testing. Pictures obtained by colposcopy were examined by indirect Bi-Digital O-Ring Test (BDORT) by using reference control substance (RCS): HPV 16, HPV 18, and Integrin α5 βl. BDORT was developed by Prof. Omura Y. of New York and received U.S. patent in 1993. For detection of HPV DNA we used RT PCR and standard Qiagen method which detect 18 types (16,18,31,33,35,39,45,51,52,56,58,59,68, 6,11,42,43,44) of HPV from smear. From 63 patients where is BDORT indicated presence of HPV, in 49 pateints (77,8%) RT PCR confirmed presence of HPV. From 63 patients in 54 patients (85,7%), we detected, by colposcopic exam, some kind of lesions associated with HPV infection. Results obtained by RT PCR: one type (1/18) of DNA HPV in 25. patients (51,02%), 2 types (2/18) in 15 patients (30,61%) and 3 types (3/18) in 9 patients (18,37%). Although BDORT results usually have higher sensitivity and detection rate is much higher, it can be used together with RT PCR in detection of HPV and cervical lesions associated with HPV infection.

Breast cancer is the most frequently diagnosed malignancy among women worldwide. There are many risk factors for breast carcinoma, such as genetics, Electro Magnetic Field Radiation (EMFs), the presence of human papillomaviruses (HPV), BRCA mutations, low vitamin D level, and toxins. The risk of developing ovarian metastases increases with the passage of time after the breast cancer diagnosis, and there are significant links between the tumor stage and the development of ovarian metastases (expressed Mammoglobin B). Because there is no screening test for ovarian cancer proven to be effective, women need to learn about their personal risk for developing ovarian cancer. This is especially true for the women already diagnosed with breast cancer, who should make sure if they are at risk for ovarian cancer. By using Bi- Digital O-Ring Test (BDORT), originated by Prof. Omura, we can detect a lot of risk factors contributing to the risk for ovarian cancer, such as the presence ofHPV in the ovary, exposure to EMFs, food intolerance, low level oftelomeres, the presence of Borrelia Burgdorferi (BB), Tuberculosis (TB) etc. A high level ofHPV 16 in the ovary that is located on the same side as breast cancer could be detected, in addition to a high level ofIntegrin aSpl, as a marker of carcinogenesis. The cervix region infected by HPV is connected with the presence of HPV on both breasts, while the infection of one ovary (either left or right) is connected with the diseased breast (with cancer) on the same side. We examined 38 patients with confirmed diagnosis of breast cancer, by using indirect BDORT and in all patients we found out one ovary with a high level of HPV infection as with high level ofIntegrin aSpl. There are some mutual factors for the carcinogenesis of breast cancer and ovary cancer (HPV infection, BB, TB etc., toxins, EMFs). All patients reported being exposed to EMFs. 29 out ofa total of38 (76.32%) patients had the infection of one ovary and the same side breast where exist breast carcinoma. In the same time on same side ovary we detected high level of HPV 16 and 18 and high level of Integrin aSpl. In three of38 patients we found out increased ovary tumor markers. In study group we found out 29 out ofa total of38 patients have a risk of ovary carcinoma. What we can learn from related literature is that ovarian carcinoma occurring after breast carcinoma is usually the metastasis of breast carcinoma; however, after this research, we believe that, in most cases, ovarian and breast carcinoma develop simultaneously, stimulated by the same or similar factors, and possibly by the same carcinoma stem cell. Copyright © 2019 Cognizant Communication Corp.

Breast cancer is the most frequently diagnosed malignancy among women worldwide. There are many risk factors for breast carcinoma, such as genetics, Electro Magnetic Field Radiation (EMFs), the presence of human papillomaviruses (HPV), BRCA mutations, low vitamin D level, and toxins. The risk of developing ovarian metastases increases with the passage of time after the breast cancer diagnosis, and there are significant links between the tumor stage and the development of ovarian metastases (expressed Mammoglobin B). Because there is no screening test for ovarian cancer proven to be effective, women need to learn about their personal risk for developing ovarian cancer. This is especially true for the women already diagnosed with breast cancer, who should make sure if they are at risk for ovarian cancer. By using Bi- Digital O-Ring Test (BDORT), originated by Prof. Omura, we can detect a lot of risk factors contributing to the risk for ovarian cancer, such as the presence ofHPV in the ovary, exposure to EMFs, food intolerance, low level oftelomeres, the presence of Borrelia Burgdorferi (BB), Tuberculosis (TB) etc. A high level ofHPV 16 in the ovary that is located on the same side as breast cancer could be detected, in addition to a high level ofIntegrin aSpl, as a marker of carcinogenesis. The cervix region infected by HPV is connected with the presence of HPV on both breasts, while the infection of one ovary (either left or right) is connected with the diseased breast (with cancer) on the same side. We examined 38 patients with confirmed diagnosis of breast cancer, by using indirect BDORT and in all patients we found out one ovary with a high level of HPV infection as with high level ofIntegrin aSpl. There are some mutual factors for the carcinogenesis of breast cancer and ovary cancer (HPV infection, BB, TB etc., toxins, EMFs). All patients reported being exposed to EMFs. 29 out ofa total of38 (76.32%) patients had the infection of one ovary and the same side breast where exist breast carcinoma. In the same time on same side ovary we detected high level of HPV 16 and 18 and high level of Integrin aSpl. In three of38 patients we found out increased ovary tumor markers. In study group we found out 29 out ofa total of38 patients have a risk of ovary carcinoma. What we can learn from related literature is that ovarian carcinoma occurring after breast carcinoma is usually the metastasis of breast carcinoma; however, after this research, we believe that, in most cases, ovarian and breast carcinoma develop simultaneously, stimulated by the same or similar factors, and possibly by the same carcinoma stem cell. Copyright © 2019 Cognizant Communication Corp.

Plants are among the oldest and most complex organisms on our world. The complexity lies, first of all, from the cellular point of view, which has a greater compartmentalization than the animal cell. Also evolved as terrestrial organisms, plants have developed complex forms of electrochemical communication, developed to the point of suggesting that plants possessed a sort of nervous system, similar to that found in the animal world and in organisms with a high degree of evolution. Over the course of history, numerous researchers, especially in the last century, have alternated trying to penetrate the functional complexity of plants, obtaining results that allowed to tip the scales towards the presence not only of a nervous system, but a complex processing capacity, comparable to a consciousness. The experiment described here, using a polygraphic apparatus connected to an artificial intelligence computerized processing system, made it possible to evaluate the physiopathological responses of a plant subjected to external stimuli of an invasive and non-invasive nature. The technique involved addressing the vegetable "directly" during the experimentation. The results obtained have highlighted a logical succession of responses, which have allowed us to conclude that plants possess an advanced capacity for processing external stimuli and also of the human voice. © 2022 Cognizant Communication Corporation. All rights reserved.

Plants are among the oldest and most complex organisms on our world. The complexity lies, first of all, from the cellular point of view, which has a greater compartmentalization than the animal cell. Also evolved as terrestrial organisms, plants have developed complex forms of electrochemical communication, developed to the point of suggesting that plants possessed a sort of nervous system, similar to that found in the animal world and in organisms with a high degree of evolution. Over the course of history, numerous researchers, especially in the last century, have alternated trying to penetrate the functional complexity of plants, obtaining results that allowed to tip the scales towards the presence not only of a nervous system, but a complex processing capacity, comparable to a consciousness. The experiment described here, using a polygraphic apparatus connected to an artificial intelligence computerized processing system, made it possible to evaluate the physiopathological responses of a plant subjected to external stimuli of an invasive and non-invasive nature. The technique involved addressing the vegetable "directly" during the experimentation. The results obtained have highlighted a logical succession of responses, which have allowed us to conclude that plants possess an advanced capacity for processing external stimuli and also of the human voice. © 2022 Cognizant Communication Corporation. All rights reserved.