Browsing by Author "Cvetkovic, Sasa (40660903300)"

Introduction: HPV is a family of double-stranded DNA viruses that infect the mucous epithelium. Most of these infections regress without consequences; however, the high-risk HPV viral strains can determine a tumor transformation of the epithelium, giving rise to tumor forms such as cervical cancer. The analysis mostly applied to define abnormalities of the membranes is the PAP test and the treatment is reconciled, in most cases through prevention. Alongside the standard protocol, we have determined that a blend of essential oils has an effect in determining the negativization of HPV. Materials and Methods: We made use of the BDORT technique in order to determine the specific percentages of each component of the prepared oil blend. To confirm this, two advanced Molecular Docking software have been applied (Swiss Dock and 1-Click Docking). The mixture of oils was subsequently applied, in the form of vaginal capsules, on a group of HPV (+) positive patients, who were tested for the presence of a virus, before and after treatment, by RT-PCR, Colposcopy, and BDORT to evaluate the state of epithelium before and after the assumption of oil vaginal capsules. Results: The mixture of oils has produced the negativization of HPV, determined by BDORT and confirmed by RT-PCR, in the treated patients. Discussion: We have determined that the blend of essential oils, prepared here, influenced the negativization of the virus, in all the patients treated. Conclusions: The operative efficacy of these oils in the negativization of HPV has been demonstrated. Copyright © 2023 Cognizant Communication Corp.

Introduction: HPV is a family of double-stranded DNA viruses that infect the mucous epithelium. Most of these infections regress without consequences; however, the high-risk HPV viral strains can determine a tumor transformation of the epithelium, giving rise to tumor forms such as cervical cancer. The analysis mostly applied to define abnormalities of the membranes is the PAP test and the treatment is reconciled, in most cases through prevention. Alongside the standard protocol, we have determined that a blend of essential oils has an effect in determining the negativization of HPV. Materials and Methods: We made use of the BDORT technique in order to determine the specific percentages of each component of the prepared oil blend. To confirm this, two advanced Molecular Docking software have been applied (Swiss Dock and 1-Click Docking). The mixture of oils was subsequently applied, in the form of vaginal capsules, on a group of HPV (+) positive patients, who were tested for the presence of a virus, before and after treatment, by RT-PCR, Colposcopy, and BDORT to evaluate the state of epithelium before and after the assumption of oil vaginal capsules. Results: The mixture of oils has produced the negativization of HPV, determined by BDORT and confirmed by RT-PCR, in the treated patients. Discussion: We have determined that the blend of essential oils, prepared here, influenced the negativization of the virus, in all the patients treated. Conclusions: The operative efficacy of these oils in the negativization of HPV has been demonstrated. Copyright © 2023 Cognizant Communication Corp.

This study presents a comparative analysis of molecular docking data, focusing on the binding interactions of the natural compounds apigenin and luteolin with the proteins TP-53, pRb, and APOBEC, in comparison to conventional pharmacological ligands. Advanced bioinformatics techniques were employed to evaluate and contrast binding energies, showing that apigenin and luteolin demonstrate significantly higher affinities for TP-53, pRb, and APOBEC, with binding energies of −6.9 kcal/mol and −6.6 kcal/mol, respectively. These values suggest strong potential for therapeutic intervention against HPV-16. Conventional ligands, by comparison, exhibited lower affinities, with energies ranging from −4.5 to −5.5 kcal/mol. Additionally, protein–protein docking simulations were performed to assess the interaction between HPV-16 E6 oncoprotein and tumor suppressors TP-53 and pRb, which revealed high binding energies around −976.7 kcal/mol, indicative of their complex interaction. A conversion formula was applied to translate these protein–protein interaction energies to a comparable scale for non-protein interactions, further underscoring the superior binding potential of apigenin and luteolin. These findings highlight the therapeutic promise of these natural compounds in preventing HPV-16-induced oncogenesis, warranting further experimental validation for clinical applications. © 2024 by the authors.

This study presents a comparative analysis of molecular docking data, focusing on the binding interactions of the natural compounds apigenin and luteolin with the proteins TP-53, pRb, and APOBEC, in comparison to conventional pharmacological ligands. Advanced bioinformatics techniques were employed to evaluate and contrast binding energies, showing that apigenin and luteolin demonstrate significantly higher affinities for TP-53, pRb, and APOBEC, with binding energies of −6.9 kcal/mol and −6.6 kcal/mol, respectively. These values suggest strong potential for therapeutic intervention against HPV-16. Conventional ligands, by comparison, exhibited lower affinities, with energies ranging from −4.5 to −5.5 kcal/mol. Additionally, protein–protein docking simulations were performed to assess the interaction between HPV-16 E6 oncoprotein and tumor suppressors TP-53 and pRb, which revealed high binding energies around −976.7 kcal/mol, indicative of their complex interaction. A conversion formula was applied to translate these protein–protein interaction energies to a comparable scale for non-protein interactions, further underscoring the superior binding potential of apigenin and luteolin. These findings highlight the therapeutic promise of these natural compounds in preventing HPV-16-induced oncogenesis, warranting further experimental validation for clinical applications. © 2024 by the authors.

Introduction: Organisms are constantly immersed in an ocean of electromagnetic waves which cause different effects on the body. At the end of 1980, it was determined that the frequency of 6.66 Hz is responsible for anxiety-depressive states and that all subjects who manifest such disturbances vibrate on this frequency. Materials and Methods: The BDORT technique was applied to evaluate parameters associated with the subject’s status (Sirtuin-1, TERT, TP-53, Zoloft, Thromboxane B2, Acetylcholine, DMAE, Thymus Gland). Furthermore, the GAP-7 and HAMD psycho-aptitude tests were applied to evaluate the subject’s anxiety-depressive level. At the end of the evaluation, the subject was treated with the OPFT (Opposite Phase Frequency Treatment), i.e., with the counter-phase frequency of -6.66 Hz, in order to cancel the 6.66 Hz frequency. The treatment was performed by listening, with headphones, to the specific anti-frequency, elaborated thanks to the combined use of artificial intelligence software. Results: Patients treated with the OPFT showed an increase, measured by BDORT, of specific biological parameters and a decrease in Thromboxane B2, in accordance with expectations, due to the balance between the sympathetic and parasympathetic systems. The data obtained, on the specific parameters, showed a statistically significant correlation (p < 0.005 ** and p < 0.001 ***). Discussion: The increase in the value of parameters Acetylcholine and the decrease in Thromboxane B2, was the basis of the alleviation of anxiety-depressive symptoms. As a corollary of them, there was an increase in parameters such as Sirtuin-1, TERT, and TP-53. Conclusions: The OPFT technique proved to be effective in reducing anxiety-depressive symptoms in all treated subjects. Copyright ©2023 Cognizant Communication Corp. Printed in the USA.

Introduction: Organisms are constantly immersed in an ocean of electromagnetic waves which cause different effects on the body. At the end of 1980, it was determined that the frequency of 6.66 Hz is responsible for anxiety-depressive states and that all subjects who manifest such disturbances vibrate on this frequency. Materials and Methods: The BDORT technique was applied to evaluate parameters associated with the subject’s status (Sirtuin-1, TERT, TP-53, Zoloft, Thromboxane B2, Acetylcholine, DMAE, Thymus Gland). Furthermore, the GAP-7 and HAMD psycho-aptitude tests were applied to evaluate the subject’s anxiety-depressive level. At the end of the evaluation, the subject was treated with the OPFT (Opposite Phase Frequency Treatment), i.e., with the counter-phase frequency of -6.66 Hz, in order to cancel the 6.66 Hz frequency. The treatment was performed by listening, with headphones, to the specific anti-frequency, elaborated thanks to the combined use of artificial intelligence software. Results: Patients treated with the OPFT showed an increase, measured by BDORT, of specific biological parameters and a decrease in Thromboxane B2, in accordance with expectations, due to the balance between the sympathetic and parasympathetic systems. The data obtained, on the specific parameters, showed a statistically significant correlation (p < 0.005 ** and p < 0.001 ***). Discussion: The increase in the value of parameters Acetylcholine and the decrease in Thromboxane B2, was the basis of the alleviation of anxiety-depressive symptoms. As a corollary of them, there was an increase in parameters such as Sirtuin-1, TERT, and TP-53. Conclusions: The OPFT technique proved to be effective in reducing anxiety-depressive symptoms in all treated subjects. Copyright ©2023 Cognizant Communication Corp. Printed in the USA.