Browsing by Author "Brasanac, Dimitrije C. (6603393153)"

Background. There is positive correlation between the number of activated hepatic stellate cells and necroinflammatory activity and/or the stage of liver fi brosis in viral hepatitis. No study has investigated such a relationship with regard to the activated hepatic cells within specifi ed zones of liver tissue in chronic C hepatitis. The aim of the present study was to correlate the level of activated hepatic stellate cells within perivenular, intermediate, periportal, and portal tracts area and fi brous septa with stages of liver fi brosis and necroinfl ammatory activity in patients with chronic C hepatitis. Methods. This retrospective study included 20 liver biopsy samples from patients with chronic C hepatitis and 10 normal liver biopsies. Biopsy specimens were processed routinely and stained with haematoxylin-eosin, periodic acid-Schiff, Masson's trichrome, aldechide fuchsin, reticulin and iron (Pearls). Activated hepatic stellate cells were identifi ed immunohistochemically using antibody to α-smooth muscle actin. Assesment of immunoreactivity was performed using a semiquantitative method. Results. In chronic C hepatitis, a positive correlation between the stage of fi brosis and the number of activated hepatic stellate cells within portal spaces and fi brous septa was found. These cells were increased in number in other areas of liver tissue as well, but without statistical signifi cance. There was no correlation between either the stage of fi brosis and necroinfl ammatory activity or the number of activated hepatic stellate cells and necroinfl ammatory activity. Conclusion. An increased number of activated hepatic stellate cells within portal spaces and fi brous septa may be a useful prognostic marker for the development of advanced fi brosis and cirrhosis in chronic C hepatitis.

BACKGROUND: The giant, invasive basal cell carcinoma of the scalp is a rare clinical form of this tumor that appears on the skin, but may spread to some of the following structures: soft tissues of the scalp, bones, meninges, and the brain. In literature, so far, it is known as the GBCC. It is caused by aggressive BCC subtypes. METHODS: We will present here a research of clinical and pathological features of 47 pathological specimens in 31 patients where the following features were examined: the dimension of the tumor, the dimension of the tissue segment, tumor area, segmentation area, resection margin width, microscopic resection margin status, tumor invasion level, and the outcome. RESULTS AND CONCLUSIONS: We have concluded that microscopic resection margin dimensions from 1 to 10 mm are safe and that relapse occurrences in giant, invasive BCCs of the scalp depend on microscopic resection margin dimensions, resection margin status, tumor invasion levels, risky occupation, and risky behavior of the patient. Copyright © 2013 Mutaz B. Habal, MD.

p300 and p300/CBP-associated factor (PCAF) are histone modifiers and transcriptional co-factors involved in a number of cell processes. We investigated their expression patterns in 79 actinic keratoses (AK), 45 cases of Bowen's disease (BD), and 168 invasive squamous cell carcinomas of the skin (SCC). Using tissue microarray and immunohistochemistry, we evaluated p300 and PCAF expression in relation to the type of the lesion and SCC prognostic parameters (grade, diameter, thickness and level of invasion). High nuclear expression of p300 (>. 60% of positive cells) (p = 0.001) and absent cytoplasmic expression (p = 0.026) were more frequent in SCC compared to AK and BD, respectively. Cytoplasmic expression of p300 was associated with the SCC invasion of subcutaneous fat and deeper tissues (p = 0.049). Diffuse distribution of cells with p300 nuclear expression was more commonly seen in BD and SCC compared to AK (p < 0.001), in moderately- and poorly-differentiated SCC compared to well-differentiated SCC (p < 0.001), in tumors thicker than 6 mm (p < 0.001), and in deeply invading tumors (p = 0.001). More frequent loss of PCAF nuclear expression was observed in SCC than in AK and BD (p < 0.001). Diffuse distribution of cells with PCAF cytoplasmic expression was more common in BD and SCC compared to AK (p < 0.001), and in poorly-differentiated SCC compared to well- and moderately-differentiated SCC (p < 0.001). Our results suggest that increase in nuclear expression of p300, as well as the presence of cytoplasmic but loss of nuclear expression of PCAF, could play an important role in the development and progression of cutaneous SCC. © 2016 Elsevier Inc..

p300 and p300/CBP-associated factor (PCAF) are histone modifiers and transcriptional co-factors involved in a number of cell processes. We investigated their expression patterns in 79 actinic keratoses (AK), 45 cases of Bowen's disease (BD), and 168 invasive squamous cell carcinomas of the skin (SCC). Using tissue microarray and immunohistochemistry, we evaluated p300 and PCAF expression in relation to the type of the lesion and SCC prognostic parameters (grade, diameter, thickness and level of invasion). High nuclear expression of p300 (>. 60% of positive cells) (p = 0.001) and absent cytoplasmic expression (p = 0.026) were more frequent in SCC compared to AK and BD, respectively. Cytoplasmic expression of p300 was associated with the SCC invasion of subcutaneous fat and deeper tissues (p = 0.049). Diffuse distribution of cells with p300 nuclear expression was more commonly seen in BD and SCC compared to AK (p < 0.001), in moderately- and poorly-differentiated SCC compared to well-differentiated SCC (p < 0.001), in tumors thicker than 6 mm (p < 0.001), and in deeply invading tumors (p = 0.001). More frequent loss of PCAF nuclear expression was observed in SCC than in AK and BD (p < 0.001). Diffuse distribution of cells with PCAF cytoplasmic expression was more common in BD and SCC compared to AK (p < 0.001), and in poorly-differentiated SCC compared to well- and moderately-differentiated SCC (p < 0.001). Our results suggest that increase in nuclear expression of p300, as well as the presence of cytoplasmic but loss of nuclear expression of PCAF, could play an important role in the development and progression of cutaneous SCC. © 2016 Elsevier Inc..