Browsing by Author "Arsic, Aleksandra (14031166400)"

Background: Recent literature data highlights metabolic changes in amyotrophic lateral sclerosis (ALS). To explore possible early metabolic changes, we aimed to analyse the fatty acids (FA) composition of erythrocytes in newly diagnosed ALS patients and to see whether fatty acid levels correlate with the ALSFRS-R score or disease duration. Methods: The severity of motor function involvement was assessed by the ALSFRS-R scale at the initial evaluation. The fatty acid profile of erythrocyte membranes was analysed by gas-liquid chromatography. The study comprised 26 clinically diagnosed ALS patients, with mean ALSFRS-R 38±8. The control group included 26 healthy volunteers. Results: Significantly higher levels of palmitic acid and total saturated FAs were detected in ALS patients. In ALS patients, total monounsaturated FA, palmitoleic, vaccenic, and oleic acid were also significantly increased. The levels of eicosapentaenoic acid, docosapentaenoic acid, total polyunsaturated FA (PUFA) and n-6 PUFA were significantly lower in ALS patients. Additionally, a-linolenic acid, the precursor of the n-3 PUFA family, was not detected in ALS patients. We found no significant correlation between the ALSFRS-R score and the abundance of individual FAs analysed. A moderate negative correlation was found between disease duration and DHA level, and a positive correlation was detected with MUFA. Conclusion: Experimental evidence presented may contribute to shaping a beneficial nutritional intervention. © 2023 Sciendo. All rights reserved.

Background: Recent literature data highlights metabolic changes in amyotrophic lateral sclerosis (ALS). To explore possible early metabolic changes, we aimed to analyse the fatty acids (FA) composition of erythrocytes in newly diagnosed ALS patients and to see whether fatty acid levels correlate with the ALSFRS-R score or disease duration. Methods: The severity of motor function involvement was assessed by the ALSFRS-R scale at the initial evaluation. The fatty acid profile of erythrocyte membranes was analysed by gas-liquid chromatography. The study comprised 26 clinically diagnosed ALS patients, with mean ALSFRS-R 38±8. The control group included 26 healthy volunteers. Results: Significantly higher levels of palmitic acid and total saturated FAs were detected in ALS patients. In ALS patients, total monounsaturated FA, palmitoleic, vaccenic, and oleic acid were also significantly increased. The levels of eicosapentaenoic acid, docosapentaenoic acid, total polyunsaturated FA (PUFA) and n-6 PUFA were significantly lower in ALS patients. Additionally, a-linolenic acid, the precursor of the n-3 PUFA family, was not detected in ALS patients. We found no significant correlation between the ALSFRS-R score and the abundance of individual FAs analysed. A moderate negative correlation was found between disease duration and DHA level, and a positive correlation was detected with MUFA. Conclusion: Experimental evidence presented may contribute to shaping a beneficial nutritional intervention. © 2023 Sciendo. All rights reserved.

Rheumatoid arthritis is a chronic inflammatory disease. Reactive oxygen species have been considered as aggravating factors for autoimmune diseases. Fatty acids had been linked in reduction of various diseases by augment of their antioxidant potential and antiinflammatory mechanisms. The aim of this study was to assess the oxidative status in patients with rheumatoid arthritis who used concentrated fish oil only or concentrated fish oil in combination with evening primrose oil in a period of 3 months. Subjects were divided into three groups. The group I consists of patients who had been taking only their regular rheumatologic therapy; group II, patients who had been taking concentrated fish oil; and group III, patients who had been taking concentrated fish oil and evening primrose oil. Peripheral blood samples were used for all the assays. We assessed the following oxidative stress markers: index of lipid peroxidation (thiobarbituric acid-reactive substances (TBARS)), hydrogen peroxide (H2O2), superoxide anion radical (O2 −), nitric oxide (NO), superoxide dismutase activity (SOD), catalase activity (CAT), and glutathione levels (GSH) in erythrocytes. There were no statistically significant changes for any of the oxidative stress parameters in group I. In group II, levels of TBARS, NO2 −, and GSH were increased, while levels of H2O2 decreased. Increased values of TBARS, NO2 −, and SOD were found in group III. Our findings indicate that intakes of fish oil and evening primrose oil may be of importance in mitigation of inflammation, disease activity, and oxidative stress biomarkers, through increased activities of antioxidant enzymes. © 2016, International League of Associations for Rheumatology (ILAR).

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The effect of intensive long-term physical activity on phospholipid fatty acid (FA) composition has not been studied thoroughly. We determined plasma and erythrocyte phospholipid FA status of professional basketball and football players. Our results showed differences in plasma FA profile not only between sportsmen and sedentary subjects, but also between two groups of sportsmen. Plasma FA profile in basketball players showed significantly higher proportion of n-6 FA (20:3, 20:4, and 22:4) and total polyunsaturated FA (PUFA) than controls, while football players had higher palmitoleic acid (16:1) than basketball players and controls. Total PUFA and 22:4 were also higher in basketball than in football players. Erythrocyte FA profile showed no differences between football players and controls. However, basketball players had higher proportion of 18:0 than controls, higher saturated FA and lower 18:2 than two other groups, and higher 22:4 than football players. These findings suggest that long-term intensive exercise and type of sport influence FA profile. © Springer-Verlag 2009.

BACKGROUND: The aim of the present study was to analyze changes in redox balance throughout parameters of oxidative stress and activities of antioxidant enzymes in elite female water polo (N.=15) and football players (N.=19) aged between 20 and 23. Fourteen age-matched sedentary women were also included in the study. METHODS: Blood sampling was performed to measure levels of lipid peroxidation (MDA), total antioxidant status (TAS), superoxide anion radical (O2), hydrogen peroxide (H2O2), reduced glutathione (GSH), oxidized glutathione (GSSG), nitrites, superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione-peroxidase activity (GPx). RESULTS: Levels of MDA, TAS, GSSG and H2O2 were significantly higher in athletes than in the control women. Football players had higher levels of O2- than the other two groups. Activity of SOD was higher in water polo players when compared with the football and control groups, CAT was increased in all athletes, while GPx did not differ among groups. CONCLUSIONS: Therefore, prolonged intensive training markedly increases oxidative stress in women, which depends on the type of sport. Lower concentration of O2- and increased activity of SOD in water polo players compared to football players suggest that mechanisms of adaptation of antioxidative defense are related to the type of exercise. © 2016 EDIZIONI MINERVA MEDICA.

BACKGROUND: The aim of the present study was to analyze changes in redox balance throughout parameters of oxidative stress and activities of antioxidant enzymes in elite female water polo (N.=15) and football players (N.=19) aged between 20 and 23. Fourteen age-matched sedentary women were also included in the study. METHODS: Blood sampling was performed to measure levels of lipid peroxidation (MDA), total antioxidant status (TAS), superoxide anion radical (O2), hydrogen peroxide (H2O2), reduced glutathione (GSH), oxidized glutathione (GSSG), nitrites, superoxide dismutase activity (SOD), catalase activity (CAT) and glutathione-peroxidase activity (GPx). RESULTS: Levels of MDA, TAS, GSSG and H2O2 were significantly higher in athletes than in the control women. Football players had higher levels of O2- than the other two groups. Activity of SOD was higher in water polo players when compared with the football and control groups, CAT was increased in all athletes, while GPx did not differ among groups. CONCLUSIONS: Therefore, prolonged intensive training markedly increases oxidative stress in women, which depends on the type of sport. Lower concentration of O2- and increased activity of SOD in water polo players compared to football players suggest that mechanisms of adaptation of antioxidative defense are related to the type of exercise. © 2016 EDIZIONI MINERVA MEDICA.

Research on possible physiological changes as a consequence of a specific lifestyle and long-term strenuous exercise in boxing has been sparse. We determined plasma and erythrocyte phospholipid (PL) fatty acids (FA) profile of 16 elite amateur male boxers (22.4±3.3 years of age), and compared them with a control group composed of 19 sedentary (24.4±3.4) year-old men. The percentages of total saturated fatty acids (SFA) and monosaturated FA in plasma phospholipids were significantly higher (P <0.001) in boxers compared to the control group. On the other hand, all studied polyunsaturated fatty acids (PUFA) in plasma PL with the exception of eicosapentaenoic acid (EPA, 20:5, n-3) and docosatetraenoic acid (DTA, 22:4, n-6) were significantly lower in boxers than in sedentary men. Total PUFA, n-6 PUFA and n-3 PUFA were also significantly lower in boxers (P <0.001), whereas the n-6/n-3 ratio was higher in boxers than in control group (P <0.01). Boxers had significantly higher proportion of all SFA in erythrocyte PL compared to the control group (P <0.05). In addition, the percentage of linoleic acid was lower in boxers' erythrocyte PL than in the control group (P <0.05). The results show two potentially unfavourable main features of the FA profile of boxers, that is, a higher n-6/n-3 ratio in plasma PL and a higher percentage of SFA in both plasma and erythrocyte phospholipids compared to controls. As SFA correlates directly with the incidence of cardiovascular disease and high n-6/n-3 ratio has been shown to stimulate carcinogenesis and modulate inflammation and autoimmunity, this profile could be detrimental to the health of boxers. The mechanism underlying these differences requires further investigation; however the results suggest benefits of nutritional intervention. © 2013 Copyright European College of Sport Science.

Research on possible physiological changes as a consequence of a specific lifestyle and long-term strenuous exercise in boxing has been sparse. We determined plasma and erythrocyte phospholipid (PL) fatty acids (FA) profile of 16 elite amateur male boxers (22.4±3.3 years of age), and compared them with a control group composed of 19 sedentary (24.4±3.4) year-old men. The percentages of total saturated fatty acids (SFA) and monosaturated FA in plasma phospholipids were significantly higher (P <0.001) in boxers compared to the control group. On the other hand, all studied polyunsaturated fatty acids (PUFA) in plasma PL with the exception of eicosapentaenoic acid (EPA, 20:5, n-3) and docosatetraenoic acid (DTA, 22:4, n-6) were significantly lower in boxers than in sedentary men. Total PUFA, n-6 PUFA and n-3 PUFA were also significantly lower in boxers (P <0.001), whereas the n-6/n-3 ratio was higher in boxers than in control group (P <0.01). Boxers had significantly higher proportion of all SFA in erythrocyte PL compared to the control group (P <0.05). In addition, the percentage of linoleic acid was lower in boxers' erythrocyte PL than in the control group (P <0.05). The results show two potentially unfavourable main features of the FA profile of boxers, that is, a higher n-6/n-3 ratio in plasma PL and a higher percentage of SFA in both plasma and erythrocyte phospholipids compared to controls. As SFA correlates directly with the incidence of cardiovascular disease and high n-6/n-3 ratio has been shown to stimulate carcinogenesis and modulate inflammation and autoimmunity, this profile could be detrimental to the health of boxers. The mechanism underlying these differences requires further investigation; however the results suggest benefits of nutritional intervention. © 2013 Copyright European College of Sport Science.