Browsing by Author "Andric, Zorana (23033104600)"

Background: Takayasu arteritis (TA) is a systemic vasculitis, affecting mainly the aorta and its branches. Objective: To analyze the HLA class I and class II alleles in patients with TA and explore their relationship with clinical and demographic characteristics, and potential significance in prognosis. Methods: Twenty-five, unrelated TA patients were genotyped for HLA-A, HLA-B, HLA-C, HLA-DRB1, and the HLA-DQB1 loci. The frequencies of the HLA-A, HLA-B, and the HLA-DRB1 were compared with a control group of 1992, while the HLA-C and the HLA-DQB1 were compared with a group of 159 healthy, unrelated individuals. Results: Among TA patients, 5/25 (20%) were identified as the HLA-B*52 carriers. There was a significant difference in the HLA-B*52 allele frequency in the TA patients (10%) compared with the healthy controls (1.2%). Moreover, presence of the HLA-B*52 was associated with significantly earlier disease onset, more severe clinical presentations, and a poorer response to treatment. The HLA-C*03 was detected in 32% of patients and was present exclusively in those with a clinically mild form of the TA, indicating a putative protective effect. Conclusion: These findings indicate that the HLA-B*52 allele contributes to a higher susceptibility to the TA whereas the HLA-C*03, can be a protective factor in the TA. © 2021, Shiraz University of Medical Sciences. All rights reserved.

Background: Takayasu arteritis (TA) is a systemic vasculitis, affecting mainly the aorta and its branches. Objective: To analyze the HLA class I and class II alleles in patients with TA and explore their relationship with clinical and demographic characteristics, and potential significance in prognosis. Methods: Twenty-five, unrelated TA patients were genotyped for HLA-A, HLA-B, HLA-C, HLA-DRB1, and the HLA-DQB1 loci. The frequencies of the HLA-A, HLA-B, and the HLA-DRB1 were compared with a control group of 1992, while the HLA-C and the HLA-DQB1 were compared with a group of 159 healthy, unrelated individuals. Results: Among TA patients, 5/25 (20%) were identified as the HLA-B*52 carriers. There was a significant difference in the HLA-B*52 allele frequency in the TA patients (10%) compared with the healthy controls (1.2%). Moreover, presence of the HLA-B*52 was associated with significantly earlier disease onset, more severe clinical presentations, and a poorer response to treatment. The HLA-C*03 was detected in 32% of patients and was present exclusively in those with a clinically mild form of the TA, indicating a putative protective effect. Conclusion: These findings indicate that the HLA-B*52 allele contributes to a higher susceptibility to the TA whereas the HLA-C*03, can be a protective factor in the TA. © 2021, Shiraz University of Medical Sciences. All rights reserved.

This study provides the first published detailed analysis of five loci polymorphisms as well as reports of two, three and five loci haplotype frequencies in the Serbian population in a sample of 1992 volunteer bone marrow donors recruited from different part of the country. Typing was performed by PCR SSO method combined with PCR SSP techniques to resolve ambiguities. In total, 16 HLA-A, 28 HLA-B, 14 HLA-C, 13 HLA-DRB1 and 5 HLA-DQB1 allelic groups were identified. The most frequent in allele groups are HLA-A*02 (29.5%), HLA-A*01 (14.2%), HLA-B*35 (13.1%), HLA-B*51 (12.8%), HLA-C*07 (24.8%), HLA-DRB1*11 (16.9%), HLA-DRB1*13 (13.2%), HLA-DQB1*03 (33.3%) and DQB1*05 (33.0%). The most frequent three- and five-loci haplotypes were A*01-B*08-DRB1*03 (5.9%) and A*02-B*18-DRB1*11 (1.9%), A*01-B*08-C*07-DRB1*03-DQB1*02 (6.6%) followed by A*02-B*18-C*07-DRB1*11-DQB1*03 (2.5%), then A*33-B*14-C*08-DRB1*01-DQB1*05 and A*02-B*35-C*04-DRB1*16-DQB1*05 (2.2% both), respectively. The results of cluster analysis showed that the Serbian population is closely related to the populations living in central Balkan and neighboring European regions. The level of allelic diversity found in this study are relevant to facilitate searching for unrelated matched donor and provide a healthy control population from our region that should be useful in the future disease association study. © 2013 American Society for Histocompatibility and Immunogenetics.

This study provides the first published detailed analysis of five loci polymorphisms as well as reports of two, three and five loci haplotype frequencies in the Serbian population in a sample of 1992 volunteer bone marrow donors recruited from different part of the country. Typing was performed by PCR SSO method combined with PCR SSP techniques to resolve ambiguities. In total, 16 HLA-A, 28 HLA-B, 14 HLA-C, 13 HLA-DRB1 and 5 HLA-DQB1 allelic groups were identified. The most frequent in allele groups are HLA-A*02 (29.5%), HLA-A*01 (14.2%), HLA-B*35 (13.1%), HLA-B*51 (12.8%), HLA-C*07 (24.8%), HLA-DRB1*11 (16.9%), HLA-DRB1*13 (13.2%), HLA-DQB1*03 (33.3%) and DQB1*05 (33.0%). The most frequent three- and five-loci haplotypes were A*01-B*08-DRB1*03 (5.9%) and A*02-B*18-DRB1*11 (1.9%), A*01-B*08-C*07-DRB1*03-DQB1*02 (6.6%) followed by A*02-B*18-C*07-DRB1*11-DQB1*03 (2.5%), then A*33-B*14-C*08-DRB1*01-DQB1*05 and A*02-B*35-C*04-DRB1*16-DQB1*05 (2.2% both), respectively. The results of cluster analysis showed that the Serbian population is closely related to the populations living in central Balkan and neighboring European regions. The level of allelic diversity found in this study are relevant to facilitate searching for unrelated matched donor and provide a healthy control population from our region that should be useful in the future disease association study. © 2013 American Society for Histocompatibility and Immunogenetics.

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