Browsing by Author "Alexopoulos, Christos G. (56806551000)"

Introduction/Objective The aim of this study was to examine the immunohistochemical features of the vascularization of the anterior cruciate ligament (ACL), as well as the quantification of capillaries within the three segments of the ACL; proximal, middle and distal. The quantification and metric characteristics of mast cells of the ACL are the second goal of this research. Methods In total, 30 human ACL of 30 persons, obtained during routine autopsy, were examined under the microscope, following immunohistochemical reactions against CD34 of blood vessels and MastTrip of mast cells. Results The middle genicular artery close to the ACL gave off branches for the supply of ligament itself. Each field of mm2 contained an average number of 1113.84 (959–1240), microvessels in ACL proximal third, an average number of 1145.43 (924–1310) microvessels in ACL middle third, and an average number of 1134.55 (889–1451) microvessels in ACL distal third. An average number of mast cells of the ACL was 3.8 per mm2. In the peripheral synovial zone of the ACL, we counted 12.6 mast cells per mm2. An average area value of the mast cells was 124.7 μm2, and an average value of shorter and longer axis of the mast cells was 11.2 × 15.0 μm. Conclusion There was no statistically significant differences between the average numbers of intraliga-mentous microvessels of the ACL thirds (p > 0.05), confirming and supporting our hypothesis of uniform distribution of blood supply within the ACL. © 2022, Serbia Medical Society. All rights reserved.

Objective. This study was conducted in order to find out whether there was any particular association between the neuronal body shapes and their immunoreactivity on substance P neurons in the supramarginal and angular gyri of inferior parietal lobule (IPL). Methods. Substance P (SP) neuropeptide was examined in the human brain (3 male and 4 female) without any neurological and psychiatric diseases, by using the method of immunohistochemistry. Immunoreactive neurons and fibers were visualized by using Olympus BT2 Camera Lucida. The collected data were statistically analyzed by using one-way analysis of variance, with the probability level p=<0.05. Results. The largest somas were obtained among SP positive neurons. Average diameters (± SD) were: longer diameter 44.93 ± 15.69 μm, shorter diameter 18.16 ± 3.77 μm. One-way analysis of variance revealed the highly significant difference among the longer axis of immunopositive neurons (p=0.002). Conclusion. A quarter of detected SP neurons were localized in lamina II of the IPL cortex. The least populated layer was lamina I (less than a tenth of all immunoreactive neurons).