Browsing by Author "Abazović, Džihan (57200380979)"

Introduction We report a case of calcification of a cervical intervertebral disk in a child. This is a rare condition, and has been described in about 400 cases worldwide. Children affected by it present with the onset of pain, muscle spasm, and the presence of calcification of a intervertebral disk on radiography. Our objective was to present a case of sudden onset of pain in the neck and torticollis. Case outline In our case, the condition was diagnosed after trauma, presented with neck pain and spasm of the right sternocleidomastoid. Initial neck radiography was done, and after identifying the calcification in front of C4 and C5 vertebral bodies, CT analysis was conducted. When it was concluded that there is no compression on spine nerve roots, conservative course of treatment was followed. The child had full regression of symptoms after two weeks. Conclusion Emergency personnel should bear in mind that, even though radiographical finding of a calcification shadow in front of the spine may raise concern, the nature of this disorder is benign in most cases, and responds very well to conservative treatment. © 2019, Serbia Medical Society. All rights reserved.

cryopreservation; cryoprotective agents; cryoinjury; stem cells; platelets. © 2023 Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.

Background: Premature ovarian failure (POF) can be found in 1% of women at the age of 35-40, mos‌tly due to un-known causes. PI3K-Akt signaling is associated with both ovarian function and growth of primordial follicles. In this s‌tudy, we examined the effects of autologous in vitro ovarian activation with s‌tem cells and autologous growth factors on reproductive and endocrine function in patients with ovarian impairment. Materials and Methods: The longitudinal prospective observational s‌tudy included 50 patients (between 30 and 50 years) with a diagnosis of POF and infertility. This multicenter s‌tudy was performed at Jevremova Special Hospital in Belgrade, Saint James Hospital (Malta), and Remedica Skoplje Hospital, between 2015 and 2018. All patients went through numerous laboratory tes‌tings, including hormonal s‌tatus. The autologous bone marrow mesenchymal s‌tem cells (BMSCs) and growth factors were used in combination for activation of ovarian tissue before its re-transplanta-tion. The software package SPSS 20.0 was used for s‌tatis‌tical analysis of the results. Results: Differences in follicle s‌timulating hormone (FSH), luteinizing hormone (LH), es‌tradiol (E2), and proges‌ter-one (PG) hormone concentrations before and after 3, 6, and 12 months pos‌t-transplantation were tes‌ted in correlation with the volume of transplanted ovarian tissue. A significant correlation (P=0.029) was found between the change in E2 level after 3 months and the volume of re-transplanted tissues. Also after re-transplantation, 64% of the patients had follicles resulting in aspiration of oocytes in 25% of positive women with follicles. Conclusion: The SEGOVA method could potentially solve many human reproductive problems in the future due to the large number of patients diagnosed with POF, as well as the possibility of delaying menopause, thus improving the quality of life and general health (Regis‌tration number: NCT04009473). © 2021, Royan Institute (ACECR). All rights reserved.

New biotechnology procedures have been introduced to the field of reproductive and perinatal medicine to find solutions for the successful reproduction, as well as disturbances responsible for maternal and perinatal morbidity. Ultrasound plays a crucial role in the early detection of reproductive disorders and perinatal medicine, where biological therapies can be possibly applied. Besides, these techniques require precise monitoring of the application of the biological agent, for which ultrasound is a sovereign method. The use of autologous sources, such as platelet-rich plasma and bone marrow-derived stem cells, provides a wide range of therapeutic strategies for gonadal failure and endometrium therapy. Stem cells from amniotic fluid could be used as the sources for direct fetal treatment in different fetal disorders (neurological disorders, the intrauterine growth restriction). Maternal complications, such as premature rupture of the amniotic sac and disturbed placental adherence, are being successfully treated with the use of biological autologous fibrin tissue adhesives. Postpartum complications related to the change of the pelvic floor could be prevented by the peripartal local application of growth factors and/or stem cells. Subcellular therapies, as microvesicles/ exosomes are membrane-bound biological nanoparticles secreted from stem cells. These particles are variable source that could act as another source of factors that could affect molecular cascades, emerging as a new diagnostic and therapeutic tool in reproductive and perinatal medicine. © The Author(s). 2020.