Browsing by Author "Ćupić, Maja (15730255400)"

Introduction/Objective The aim of this study was to investigate the distribution of genotypes and alleles of proinflammatory cytokines TNF, IFN-γ, and IL-12 and their effect on the development of a cervical ill-ness and also to determine their associated influence with cofactors in HR HPV-positive women in Serbia. Methods We have investigated 24 women and based on the cytological findings they were classified into four groups: PAP II, ASCUS, LSIL, and HSIL. Analysis of TNF, IL-12, and IFN-γ polymorphisms was performed using the real-time PCR TaqMan method. Statistical analysis was performed using parametric and non-parametric tests and correlation and multiple regression analysis. Results Significantly higher frequency of high production-related TNF AA genotype was observed in severe dysplasia. The correlation between TNF gene polymorphism and cervical findings were highly significant. There was a moderate, significant correlation between low production IFN-γ AA genotype and earlier cervical infections. There was a significant correlation between the IL-12 polymorphism of the low production IL-12 AA genotype and cervical lesions. Conclusion Results of this study show that HSIL is associated with significantly higher frequency of high production TNF AA genotype. It is known that polymorphisms of certain cytokine genes encoding proteins involved in Th1 and Th2 cellular responses may be associated with better or worse prognosis of cervical disease in women with persistent HR HPV infection. Therefore, they may be considered as biomarkers that may have a predictive role in the development of cervical cancer. © 2022, Serbia Medical Society. All rights reserved.

Objectives: The human cytomegalovirus is a notorious pathogen in the pediatric transplant setting. Although studies on factors in complicity with cytomegalovirus infection abound, the roles of age, sex, allogeneic hematopoietic stem cell transplant modality, and type of underlying disease (malignant vs nonmalignant) with regard to cytomegalovirus infection and viral load in children are seldom explored. Our aim was to examine the significance of these factors on cytomegalovirus infection and viral load in Serbian pediatric recipients of allogeneic hematopoietic stem cell transplant. Materials and Methods: Thirty-two pediatric recipients of allogeneic hematopoietic stem cell transplant to treat various malignant and nonmalignant disorders were prospectively monitored for cytomegalovirus infection. The real-time quantitative polymerase chain reaction was used for pathogen detection and quantitation. Demographic and virologic parameters were statistically analyzed with SPSS statistics software (version 20). Results: Cytomegalovirus DNA was detected in 23 patients (71.9%). Infection occurred significantly more often (P = .015) in patients with haploidentical donors. The opposite was noted for matched sibling grafts (P = .006). Viral load was higher in female patients (P = .041) and children with malignant diseases (P = .019). There was no significant relationship between viral infection or load and medical complications. Conclusions: Transplant recipients presented with a high incidence of cytomegalovirus viremia. The modality of allogeneic hematopoietic stem cell transplant was associated with the frequency of cytomegalovirus infection. Age, sex, type of underlying disease, and medically relevant events were not conducive to occurrences of viremia. Notably, we observed substantial viral loads in female patients and patients with neoplastic diseases. Studies comprising larger populations are needed to better understand these results. © Başkent University 2021 Printed in Turkey. All Rights Reserved.

We present two cases of acute myeloid leukemia (AML) who developed Varicella-Zoster Virus (VZV) encephalitis after completion of standard "3 + 7" induction remission chemotherapy. A 50-year-old patient developed disseminated cutaneous Herpes Zoster (HZ) 2 days after completion of induction chemotherapy for AML. The patient was treated with intravenous acyclovir 7 days and then orally. On the second day following intravenous acyclovir discontinuation confusion, cerebelar ataxia somnolescence and VZV encephalitis was diagnosed. The other 38-year-old patient developed neuroleukemia and VZV encephalitis without skin rash after completion of induction chemotherapy. In both patients the diagnosis was confirmed by polymerase chain reaction (PCR) for VZV DNA in serum and liquor. The first patient completely recovered after reinstitution of intravenous acyclovir while the other patient a month later. This is unusual presentation of VZV encephalitis occurring in a patient with AML after standard induction remission chemotherapy which implies the significance of early diagnosis and screening for viral infections in AML patients with unusual neurologic presentation even in absence of rash. The screening for viral infections should be performed because antiviral prophylaxis is not routinely recommended for AML during standard induction chemotherapy by most clinical guidelines. © 2013 Elsevier Masson SAS.

We present two cases of acute myeloid leukemia (AML) who developed Varicella-Zoster Virus (VZV) encephalitis after completion of standard "3 + 7" induction remission chemotherapy. A 50-year-old patient developed disseminated cutaneous Herpes Zoster (HZ) 2 days after completion of induction chemotherapy for AML. The patient was treated with intravenous acyclovir 7 days and then orally. On the second day following intravenous acyclovir discontinuation confusion, cerebelar ataxia somnolescence and VZV encephalitis was diagnosed. The other 38-year-old patient developed neuroleukemia and VZV encephalitis without skin rash after completion of induction chemotherapy. In both patients the diagnosis was confirmed by polymerase chain reaction (PCR) for VZV DNA in serum and liquor. The first patient completely recovered after reinstitution of intravenous acyclovir while the other patient a month later. This is unusual presentation of VZV encephalitis occurring in a patient with AML after standard induction remission chemotherapy which implies the significance of early diagnosis and screening for viral infections in AML patients with unusual neurologic presentation even in absence of rash. The screening for viral infections should be performed because antiviral prophylaxis is not routinely recommended for AML during standard induction chemotherapy by most clinical guidelines. © 2013 Elsevier Masson SAS.