Trivellin, Giampaolo (36501022100)Giampaolo (36501022100)TrivellinButz, Henriett (26631703100)Henriett (26631703100)ButzDelhove, Juliette (55360336600)Juliette (55360336600)DelhoveIgreja, Susana (24344029100)Susana (24344029100)IgrejaChahal, Harvinder S. (6603250500)Harvinder S. (6603250500)ChahalZivkovic, Vladimir (36783131300)Vladimir (36783131300)ZivkovicMcKay, Tristan (7005835387)Tristan (7005835387)McKayPatócs, Attila (6602069527)Attila (6602069527)PatócsGrossman, Ashley B. (35401342800)Ashley B. (35401342800)GrossmanKorbonits, Márta (7004190977)Márta (7004190977)Korbonits2025-06-122025-06-122012https://doi.org/10.1152/ajpendo.00546.2011https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866412294&doi=10.1152%2fajpendo.00546.2011&partnerID=40&md5=afd9d624afdc8f737d8c25db2e000ad5https://remedy.med.bg.ac.rs/handle/123456789/9499Abnormal microRNA (miRNA) expression profiles have recently been associated with sporadic pituitary adenomas, suggesting that miRNAs can contribute to tumor formation; miRNAs are small noncoding RNAs that inhibit posttranscriptional expression of target mRNAs by binding to target sequences usually located in the 3'-UTR. In this study, we investigated the role played by miR-107, a miRNA associated with different human cancers, in sporadic pituitary adenomas and its interaction with the pituitary tumor suppressor gene aryl hydrocarbon receptor-interacting protein (AIP). miR-107 expression was evaluated in pituitary adenoma and normal pituitary samples using microRNA screen TLDA (TaqMan Low-Density Array) and RT-qPCR assays. We show that miR-107 expression was significantly upregulated in GH-secreting and nonfunctioning pituitary adenomas. We found that human AIP-3'-UTR is a target of miR-107 since miR-107 inhibited in vitro AIP expression to 53.9 + 2% of the miRNA control in a luciferase assay and reduced endogenous AIP mRNA expression to 53 + 22% of the miRNA control in human cells. However, we did not observe a negative correlation between AIP and miR-107 expression in the human tumor samples. Furthermore, we show that miR-107 overexpression inhibited cell proliferation in human neuroblastoma and rat pituitary adenoma cells. In conclusion, miR-107 is overexpressed in pituitary adenomas and may act as a tumor suppressor. We have identified and confirmed AIP as a miR-107 target gene. Expression data in human samples suggest that the expression of AIP and miR-107 could be influenced by a combination of tumorigenic factors as well as compensatory mechanisms stimulated by the tumorigenic process. © 2012 the American Physiological Society.miR-107Pituitary adenoma