Čokić, Vladan P. (6507196877)Vladan P. (6507196877)ČokićMojsilović, Slavko (14036036900)Slavko (14036036900)MojsilovićJauković, Aleksandra (7006010128)Aleksandra (7006010128)JaukovićKraguljac-Kurtović, Nada (37037758700)Nada (37037758700)Kraguljac-KurtovićMojsilović, Sonja (57197100252)Sonja (57197100252)MojsilovićŠefer, Dijana (6603146747)Dijana (6603146747)ŠeferMitrović Ajtić, Olivera (56586150800)Olivera (56586150800)Mitrović AjtićMilošević, Violeta (24399200100)Violeta (24399200100)MiloševićBogdanović, Andrija (6603686934)Andrija (6603686934)BogdanovićDikić, Dragoslava (42061363200)Dragoslava (42061363200)DikićMilenković, Pavle (7006080567)Pavle (7006080567)MilenkovićPuri, Raj K. (7202045715)Raj K. (7202045715)Puri2025-07-022025-07-022015https://doi.org/10.1016/j.bcmd.2015.08.002https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943534715&doi=10.1016%2fj.bcmd.2015.08.002&partnerID=40&md5=cfeb11656d7ee0136a66c6a938988f70https://remedy.med.bg.ac.rs/handle/123456789/13514Purpose: We compared the gene expression profile of peripheral blood CD34+ cells and granulocytes in subjects with chronic myeloid leukemia (CML), with the accent on signaling pathways affected by BCR-ABL oncogene. Methods: The microarray analyses have been performed in circulating CD34+ cells and granulocytes from peripheral blood of 7 subjects with CML and 7 healthy donors. All studied BCR-ABL positive CML patients were in chronic phase, with a mean value of 2012±SD of CD34+cells/μl in peripheral blood. Results: The gene expression profile was more prominent in CML CD34+ cells (3553 genes) compared to granulocytes (2701 genes). The 41 and 39 genes were significantly upregulated in CML CD34+ cells (HINT1, TXN, SERBP1) and granulocytes, respectively. BCR-ABL oncogene activated PI3K/AKT and MAPK signaling through significant upregulation of PTPN11, CDK4/6, and MYC and reduction of E2F1, KRAS, and NFKBIA gene expression in CD34+ cells. Among genes linked to the inhibition of cellular proliferation by BCR-ABL inhibitor Imatinib, the FOS and STAT1 demonstrated significantly decreased expression in CML. Conclusion: The presence of BCR-ABL fusion gene doubled the expression quantity of genes involved in the regulation of cell cycle, proliferation and apoptosis of CD34+ cells. These results determined the modified genes in PI3K/AKT and MAPK signaling of CML subjects. © 2015 Elsevier Inc.CD34⁺ cellsChronic myeloid leukemiaGranulocytesMicroarray analysis