Stamenkovic, Miroslav (7003436370)Miroslav (7003436370)StamenkovicRadic, Tanja (35275858300)Tanja (35275858300)RadicStefanovic, Ivan (25628694100)Ivan (25628694100)StefanovicCoric, Vesna (55584570400)Vesna (55584570400)CoricSencanic, Ivan (55376191500)Ivan (55376191500)SencanicPljesa-Ercegovac, Marija (16644038900)Marija (16644038900)Pljesa-ErcegovacMatic, Marija (58618962300)Marija (58618962300)MaticJaksic, Vesna (23667666000)Vesna (23667666000)JaksicSimic, Tatjana (6602094386)Tatjana (6602094386)SimicSavic-Radojevic, Ana (16246037100)Ana (16246037100)Savic-Radojevic2025-06-122025-06-122014https://doi.org/10.1111/ceo.12180https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899095688&doi=10.1111%2fceo.12180&partnerID=40&md5=3cba56eee385552cde890f79dda34651https://remedy.med.bg.ac.rs/handle/123456789/8806Background: Glutathione S-transferase omega-1 and 2 have a unique range of enzymatic activities, including the regeneration of ascorbate by their dehydroascorbate reductase activities. Because these enzymes could have a protective role from oxidative damage in the lens, the question of whether the two coding glutathione S-transferase omega polymorphisms confer the risk of age-related cataract was addressed. Methods: rs4925 (Ala140Asp) of glutathione S-transferase omega-1 and rs156697 (Asn142Asp) of glutathione S-transferase omega-2 polymorphisms in 100 patients with age-related cataract and 130 controls were assessed. Results: Presence of one mutant GSTO1*Asp or GSTO2*Asp allele did not contribute independently towards the risk of cataract; however, homozygous carriers of GSTO1*Asp/GSTO2*Asp haplotype demonstrated 3.42-fold enhanced risk of cataract development (95% confidence interval=0.84-13.93; P=0.086). When GSTO genotype was analysed in association with smoking or professional exposure to ultraviolet irradiation, carriers of at least one mutant GSTO2*Asp allele had increased risk of cataract development in comparison with individuals with wild-type GSTO2*Asn/Asn with no history of smoking or ultraviolet exposure (odds ratio=6.89, 95% confidence interval=1.81-16.21, P=0.005; odds ratio=4.10, 95% confidence interval=1.23-13.74, P=0.022, respectively). Regarding the distribution of particular glutathione S-transferase omega genotype and cataract type, the highest frequency of mutant GSTO2*Asp allele was found in patients with nuclear cataract. Conclusion: The results indicate that mutant GSTO2*Asp genotype is associated with increased risk of age-related cataract in smokers and ultraviolet-exposed subjects, suggesting a role of inefficient ascorbate regeneration in cataract development. © 2014 Royal Australian and New Zealand College of Ophthalmologists.AssociationDisease riskGSTO gene