Lazarević, Milica (57204644899)Milica (57204644899)LazarevićDjedovic, Neda (54902044600)Neda (54902044600)DjedovicStanisavljević, Suzana (56545525300)Suzana (56545525300)StanisavljevićDimitrijević, Mirjana (56268210300)Mirjana (56268210300)DimitrijevićStegnjaić, Goran (57222587368)Goran (57222587368)StegnjaićKrishnamoorthy, Gurumoorthy (14421017300)Gurumoorthy (14421017300)KrishnamoorthyMostarica Stojković, Marija (6701741422)Marija (6701741422)Mostarica StojkovićMiljković, Đorđe (7006524033)Đorđe (7006524033)MiljkovićJevtić, Bojan (57191532541)Bojan (57191532541)Jevtić2025-07-022025-07-022021https://doi.org/10.1016/j.jneuroim.2021.577547https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103341915&doi=10.1016%2fj.jneuroim.2021.577547&partnerID=40&md5=182727cf38d2ec0e84153282ca5418f2https://remedy.med.bg.ac.rs/handle/123456789/12325Experimental autoimmune encephalomyelitis (EAE) is classically induced with complete Freund's adjuvant (CFA). The immune response against CFA has a confounding influence on the translational capacity of EAE as a multiple sclerosis model. Here, we compare clinical, cellular and molecular properties between syngeneic spinal cord homogenate (SCH)- and SCH + CFA-immunized Dark Agouti rats. EAE signs were observed earlier and the cumulative clinical score was higher without CFA. Also, a higher number of immune cells infiltrates in the spinal cords was noticed at the peak of EAE without CFA. High spinal cord abundance of CD8+CD11bc+MHC class II+ cells was detected in SCH-immunized rats. Myelin basic protein -specific response can be elicited in the cells from the lymph nodes draining the site of SCH immunization. This CFA-free EAE is a reliable multiple sclerosis model. © 2021 Elsevier B.V.CD4⁺ T cellsCD8⁺ macrophagesCFAEAE