Ypsilantis, Petros (6603429809)Petros (6603429809)YpsilantisLambropoulou, Maria (56234594400)Maria (56234594400)LambropoulouMilicevic, Miroslav (7005565664)Miroslav (7005565664)MilicevicBulajic, Predrag (35615774800)Predrag (35615774800)BulajicKarayiannakis, Anastasios (57202315331)Anastasios (57202315331)KarayiannakisZacharoulis, Dimitrios (6603328970)Dimitrios (6603328970)ZacharoulisSimopoulos, Constantinos (35452248900)Constantinos (35452248900)Simopoulos2025-06-122025-06-122017https://doi.org/10.1002/jhbp.513https://www.scopus.com/inward/record.uri?eid=2-s2.0-85033774178&doi=10.1002%2fjhbp.513&partnerID=40&md5=4d1d733e4a255cfaba2ea18cb2615b3fhttps://remedy.med.bg.ac.rs/handle/123456789/6745Background: The aim of the present study was to microscopically assess the tissue-sparing potential of contemporary radiofrequency-assisted liver resection (RF-LR) techniques. Methods: Twenty-four pigs were subjected to either (1) partial hepatectomy (PH) using the sequential-coagulate-cut (SCC) technique (group SCC, n = 6) using a monopolar electrode, the technique using the bipolar electrode Habib-4X (group H, n = 6) or the “crush-clamp” technique (group CC, n = 6); or (2) sham operation (group Sham, n = 6). At 48 h post-operation, liver parenchyma proximal to the ablation rim was excised for histopathologic examination and immunohistochemical assessment of apoptosis (antibody M30) and inflammatory response (antibodies IL-6, TNFα and NFκB). Results: Histopathologic index increased from the 1st to the 4th, the 1st to the 2nd or only the 1st cm from the inner margin of the ablation rim in group SCC, H or CC, respectively. The index was higher in group SCC compared to the other groups. Tissue expression of M30, IL-6, TNFα and NFκB increased in all PH groups, being higher and more expanded in group SCC, H, SCC and SCC, respectively. Conclusions: RF-LR techniques had variable microscopically assessed tissue-sparing effect. The Habib-4X proved to be less injurious compared to the SCC Belgrade technique regarding the severity and extent of tissue damage proximal to the ablation rim. © 2017 Japanese Society of Hepato-Biliary-Pancreatic SurgeryLiver resectionRadiofrequencyTissue injury