Vázquez-Lorente, Héctor (57215341073)Héctor (57215341073)Vázquez-LorenteDunerović, Duško M. (58113154500)Duško M. (58113154500)DunerovićTatić, Svetislav B. (6701763955)Svetislav B. (6701763955)TatićRadojević-Škodrić, Sanja (15726145200)Sanja (15726145200)Radojević-ŠkodrićGomes, Cláudio M. (7101707123)Cláudio M. (7101707123)GomesPaunović, Ivan R. (55990696700)Ivan R. (55990696700)PaunovićDragutinović, Vesna (6508345286)Vesna (6508345286)Dragutinović2025-07-022025-07-022023https://doi.org/10.1097/PAI.0000000000001092https://www.scopus.com/inward/record.uri?eid=2-s2.0-85148307432&doi=10.1097%2fPAI.0000000000001092&partnerID=40&md5=27ae03cf34dd44ad85131a480de984d9https://remedy.med.bg.ac.rs/handle/123456789/11890Medullary Thyroid Carcinoma (MTC) is a tumor of the neuroendocrine system. In recent years, the need to assess the MTC diagnostic-related parameters has emerged with the aim to elucidate the mechanisms involved in this pathology. The objective of this study was to evaluate the role of Matrix Metalloproteinases (MMPs) 2 and 9, their tissue inhibitors of matrix metalloproteinases (TIMPs), S100 protein, and amyloid in the diagnostic of MTC. Thirty-two samples with MTC (72% women) were included in this cross-sectional study and divided by groups: T category 1 (T1)≤20 mm and T category 2 (T2) 20 to 40 mm of tumor size. MMPs 2 and 9, TIMPs 2 and 1, S100 protein, and calcitonin in tissues were obtained by immunohistochemical techniques. The presence of amyloid in tissue sections was detected on Thioflavin T-stained slides under fluorescent microscope. Percentage of positive cells (P) observed for MMP-2 was higher in those samples presenting T2 MTC with respect to those with T1 MTC (P<0.05). Moreover, P-MMP-2 showed a direct correlation with higher T category of MTC (Rho=0.439, P < 0.001), whereas P-MPP-9 was directly correlated with S100 protein and the intensity of calcitonin in tissues (Rho=0.419, P=0.017; Rho=0.422, P=0.016, respectively. Therefore, MMPs were directly correlated with some traditional biomarkers of MTC. In this regard, P-MMP-2 was more expressed in type 2 MTC. Combining the analysis of traditional and other useful biomarkers of MTC as MMPs 2 and 9 could be a useful strategy in the diagnostic of MTC. © 2023 Lippincott Williams and Wilkins. All rights reserved.amyloidmatrix metalloproteinases (MMPs)medullary thyroid carcinomaS100 proteintissue inhibitors of matrix metalloproteinases (TIMPs)