Zeljic, Katarina (37035590000)Katarina (37035590000)ZeljicPavlovic, Dunja (59532676000)Dunja (59532676000)PavlovicStojkovic, Goran (59498807300)Goran (59498807300)StojkovicDragicevic, Sandra (57189326579)Sandra (57189326579)DragicevicLjubicic, Jelena (57209233078)Jelena (57209233078)LjubicicTodorovic, Nikola (57810532000)Nikola (57810532000)TodorovicNikolic, Aleksandra (57194842918)Aleksandra (57194842918)Nikolic2025-07-022025-07-022025https://doi.org/10.1111/jop.13606https://www.scopus.com/inward/record.uri?eid=2-s2.0-85216339687&doi=10.1111%2fjop.13606&partnerID=40&md5=4d13570add2549ee6d00b6091679aacbhttps://remedy.med.bg.ac.rs/handle/123456789/11417Introduction: A recent pan-cancer transcriptome analysis indicated differential activity of alternative promoters of genes TNS3 and LRRFIP1 in head and neck squamous cell carcinoma compared to non-cancerous tissue. The promoters upregulated in head and neck squamous cell carcinoma regulate expression of transcripts TNS3-203 and LRRFIP1-211. Objective: Our aim was to investigate the biomarker potential of TNS3-203 and LRRFIP1-211 transcripts in oral cancer, the most common type of head and neck cancer. Materials and Methods: An in silico approach was used to characterize the promoters and transcripts of interest. Relative expression of TNS3-203 and LRRFIP1-211 transcripts was evaluated by qRT-PCR in a group of 46 oral cancer patients in samples of cancer and adjacent non-cancerous tissue. Results: TNS3-203 was significantly overexpressed in oral cancer compared with matched non-cancerous tissue, so this transcript can potentially be used as a diagnostic biomarker. There were no differences in LRRFIP1-211 level between analyzed tissues. None of the investigated transcripts has prognostic potential in oral cancer. Conclusion: The results obtained indicate the diagnostic potential of TNS3-203, but not LRRFIP1-211 transcript and its role in oral carcinogenesis. © 2025 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.biomarkergene promoterLRRFIP1oral cancerTNS3transcript