Popadic, Svetlana (24830928800)Svetlana (24830928800)PopadicSavic, Emina (24822544200)Emina (24822544200)SavicMarkovic, Milos (7101935774)Milos (7101935774)MarkovicRamic, Zorica (6603943950)Zorica (6603943950)RamicMedenica, Ljiljana (16744100000)Ljiljana (16744100000)MedenicaPravica, Vera (7003322504)Vera (7003322504)PravicaSpuran, Zorica (55389309000)Zorica (55389309000)SpuranTrajkovic, Vladimir (7004516866)Vladimir (7004516866)TrajkovicPopadic, Dusan (6602255798)Dusan (6602255798)Popadic2025-06-122025-06-122015https://doi.org/10.5021/ad.2015.27.2.128https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925600005&doi=10.5021%2fad.2015.27.2.128&partnerID=40&md5=1113470a4a9e8137a174d3ffa744c66dhttps://remedy.med.bg.ac.rs/handle/123456789/8261Background: Psoriasis is a common chronic inflammatory skin disease with a strong genetic basis. Cytokines such as tumor necrosis factor alpha (TNF-α), interleukins (ILs) such are IL-12 and IL-23, and interferon gamma (IFN-γ) are released from various inflammatory and resident cells, and have been implicated in the initiation/maintenance of inflammation. Certain alleles of the aforementioned cytokines may be associated with disease susceptibility/severity. Objective: To investigate the association of three common functional gene polymorphisms, namely TNF -308 G/A (rs1800629), IL12B (encoding the p40 subunit of IL-12/23) +1188 A/C (rs3212227), and IFNG +874 T/A (rs2430561) with psoriasis development and severity in Serbian patients. Methods: We genotyped 130 patients with psoriasis (26 of whom also had psoriatic arthritis) and 259 controls; rs1800629 and rs3212227, and rs2430561, by real-time PCR assay. Results: The TNF GG genotype was detected at a higher frequency in patients with psoriasis compared to control subjects (OR, 1.420; 95% CI, 0.870∼2.403) without statistical significance (p= 0.191). Lack of the TNF G allele was associated with lower psoriasis severity (p=0.007). The IL12B AC genotype was underrepresented in the patients with psoriatic arthritis compared to healthy subjects (OR, 0.308; 95% CI, 0.090∼1.057; p=0.049). The distribution of the rs2430561 allele and genotype frequencies was similar between patients with psoriasis and controls. Conclusion: Our study demonstrates an effect of the rs1800629 on psoriasis severity, and a marginal impact of the rs3212227 on susceptibility to psoriatic arthritis. Collectively, our results obtained in a Serbian cohort expand current knowledge regarding individual predisposition to psoriatic disease.Allele frequenciesInterferon gammaP40 IL-12/23PsoriasisReal-time PCRTumor necrosis factor-alpha