Bogavac-Stanojevic, Natasa (6506171691)Natasa (6506171691)Bogavac-StanojevicStevuljevic, Jelena Kotur (36629424300)Jelena Kotur (36629424300)StevuljevicCerne, Darko (6603317701)Darko (6603317701)CerneZupan, Janja (24553108300)Janja (24553108300)ZupanMarc, Janja (7006701288)Janja (7006701288)MarcVujic, Zorica (6602596481)Zorica (6602596481)VujicCrevar-Sakac, Milkica (55635840800)Milkica (55635840800)Crevar-SakacSopic, Miron (55807303500)Miron (55807303500)SopicMunjas, Jelena (57194078742)Jelena (57194078742)MunjasRadenkovic, Miroslav (7005551185)Miroslav (7005551185)RadenkovicJelic-Ivanovic, Zorana (6603775254)Zorana (6603775254)Jelic-Ivanovic2025-07-022025-07-022018https://doi.org/10.1080/13880209.2018.1434549https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046750511&doi=10.1080%2f13880209.2018.1434549&partnerID=40&md5=19b89e8edfca96c640ee5efc737fdeeahttps://remedy.med.bg.ac.rs/handle/123456789/13036Context: Polyphenols and flavonoids in artichoke leaf tincture (ALT) protect cells against oxidative damage. Objectives: We examined ALT effects on deoxyribonucleic acid (DNA) damage and lipid profiles in rat plasma and gene expression in rat aorta [haemeoxygenase-1 (HO1), haemeoxygenase-2 (HO2), NADPH oxidase 4 (NOX-4), monocyte chemoattractant protein-1 (MCP-1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2)]. Materials and methods: Eighteen male Wistar albino rats were divided into three groups (n=6/group): The control group (CG) was fed with standard pellet chow for 11 weeks; the AD group was fed for a similar period of time with pellet chow supplemented with 2% cholesterol, 3% sunflower oil and 1% sodium cholate. The ADA group was fed with pellet chow (for 1 week), the atherogenic diet (see above) for the following 4 weeks and then with ALT (0.1 mL/kg body weight) and atherogenic diet for 6 weeks. According to HPLC analysis, the isolated main compounds in ALT were chlorogenic acid, caffeic acid, isoquercitrin and rutin. Results: Normalized HO-1 [0.11 (0.04-0.24)] and MCP-1 [0.29 (0.21-0.47)] mRNA levels and DNA scores [12.50 (4.50-36.50)] were significantly lower in the ADA group than in the AD group [0.84 (0.35-2.51)], p=0.021 for HO-1 [0.85 (0.61-3.45)], p=0.047 for MCP-1 and [176.5 (66.50-221.25)], p=0.020 for DNA scores. HO-1 mRNA was lower in the ADA group than in the CG group [0.30 (0.21-0.71), p=0.049]. Conclusions: Supplementation with ALT limited the effects of the atherogenic diet through reduced MCP-1 expression, thereby preventing oxidative damage. © 2018 The Author(s).Gene expressionHaemeoxygenase-1Monocyte chemoattractant protein-1Oxidative damage