Mijajlović, Vladimir (58771884500)Vladimir (58771884500)MijajlovićMiler, Marko (55926151300)Marko (55926151300)MilerIlić, Rosanda (56688276500)Rosanda (56688276500)IlićRašić, Dejan (24400176900)Dejan (24400176900)RašićDunđerović, Duško (56515503700)Duško (56515503700)DunđerovićRaičević, Savo (56176851100)Savo (56176851100)RaičevićSoldatović, Ivan (35389846900)Ivan (35389846900)SoldatovićDe Luka, Silvio (56957018200)Silvio (56957018200)De LukaManojlović-Gačić, Emilija (36439877900)Emilija (36439877900)Manojlović-Gačić2025-07-022025-07-022024https://doi.org/10.1007/s11060-023-04532-yhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85180210109&doi=10.1007%2fs11060-023-04532-y&partnerID=40&md5=0b335ec0c32543651169bba0143d0a69https://remedy.med.bg.ac.rs/handle/123456789/11755Purpose: Meningiomas are tumours originating from meningothelial cells, the majority belonging to grade 1 according to the World Health Organization classification of the tumours of the Central Nervous System. Factors contributing to the progression to the higher grades (grades 2 and 3) have not been elucidated yet. Senescence has been proposed as a potential mechanism constraining the malignant transformation of tumours. Senescence-associated beta-galactosidase (SA-β-GAL) and inhibitors of cyclin-dependent kinases p16 and p21 have been suggested as senescence markers. Methods: We analysed 318 meningiomas of total 343 (178 grade 1, 133 grade 2 and 7 grade 3). Tissue microarrays were constructed and stained immunohistochemically, using antibodies for SA-β-GAL, p16 and p21. Results: The positive correlation of the tumour grade with the expression of p16 (p = 0.016) and SA-β-GAL (p = 0.002) was observed. The expression of p16 and SA-β-GAL was significantly higher in meningiomas grade 2 compared to meningiomas grade 1 (p = 0.006 and p = 0.004, respectively). SA-β-GAL positivity positively correlated with p16 and p21 in the whole cohort. In grade 2 meningiomas, a positive correlation was only between SA-β-GAL and p16. Correlations of senescence markers in meningiomas grade 2 were not present. Conclusion: Our findings suggest the senescence activation in meningiomas grade 2 as a potential mechanism for the restraining of tumour growth and give hope for applying of promising senolytic therapy. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.Atypical meningiomaBeta-galactosidaseGrade 2 meningiomap16p21Senescence