Samardzic, Tatjana (6602855000)Tatjana (6602855000)SamardzicStosic-Grujicic, Stanislava (7004253020)Stanislava (7004253020)Stosic-GrujicicRaicevic, Nevena (35333437200)Nevena (35333437200)RaicevicTrajkovic, Vladimir (7004516866)Vladimir (7004516866)Trajkovic2025-07-022025-07-022001https://doi.org/10.1016/S1567-5769(01)00014-5https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035103116&doi=10.1016%2fS1567-5769%2801%2900014-5&partnerID=40&md5=74c388a483d43947412ebfbec986b942https://remedy.med.bg.ac.rs/handle/123456789/14481The effects of tiazofurin (TR) on proliferation and cytokine-induced nitric oxide (NO) production in the L929 fibrosarcoma cell line and murine embryonic fibroblasts were investigated. Treatment with TR inhibited the growth of nonconfluent L929 cells in a dose-dependent manner. TR, at concentrations not affecting cell viability or proliferation, markedly decreased IFN-γ + LPS-induced expression of inducible NO synthase (iNOS) mRNA and, subsequently, NO production in confluent L929 cultures. However, TR did not interfere with the IFN-γ-triggered expression of mRNA for IRF-1, an important iNOS transcription factor, implying that TR interferes with some other intracellular pathway involved in iNOS induction triggered by IFN-γ + LPS. In contrast to the results obtained in L929 cells, iNOS mRNA expression induced by IFN-γ + LPS in murine embryonic fibroblasts was resistant to TR, indicating a tumor-selective action of this agent. © 2001 Elsevier Science B.V.FibroblastNitric oxideTiazofurinTumor