Šefer, Dijana (6603146747)Dijana (6603146747)ŠeferMiljić, Predrag (6604038486)Predrag (6604038486)MiljićKraguljac-Kurtović, Nada (37037758700)Nada (37037758700)Kraguljac-KurtovićBižić-Radulović, Sandra (57192677013)Sandra (57192677013)Bižić-RadulovićBogdanović, Andrija (6603686934)Andrija (6603686934)BogdanovićKnežević, Vesna (56806620700)Vesna (56806620700)KneževićMarković, Dragana (24426339600)Dragana (24426339600)MarkovićBeleslin-Čokić, Bojana (6506788366)Bojana (6506788366)Beleslin-ČokićNovaković, Ivana (6603235567)Ivana (6603235567)NovakovićMarinković, Jelena (7004611210)Jelena (7004611210)MarinkovićLeković, Danijela (36659562000)Danijela (36659562000)LekovićGotić, Mirjana (7004685432)Mirjana (7004685432)GotićČokić, Vladan (6507196877)Vladan (6507196877)Čokić2025-07-022025-07-022022https://doi.org/10.1111/ijlh.13754https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118718507&doi=10.1111%2fijlh.13754&partnerID=40&md5=80fb0f610ce86f688ce7f97ca7690fc9https://remedy.med.bg.ac.rs/handle/123456789/12091Introduction: The impact of activated blood and endothelial cells on the thrombosis in myeloproliferative neoplasms (MPN) has not yet been clarified. We prospectively analyzed correlation between circulating leukocyte-platelet aggregates and soluble selectins to thrombosis occurrence in MPN, in the context of standard and cardiovascular risk factors, and different clinical and biological characteristics. Methods: Flow cytometric analysis of neutrophil-platelet (Neu-Plt) and monocyte-platelet (Mo-Plt) aggregates in peripheral blood, as well as quantification of soluble E-/L-/P-selectins by enzyme immunoassay, was performed on 95 newly diagnosed MPN patients. Results: During the follow-up, thrombosis occurred in 12.6% MPN patients (arterial 9.4%, venous 3.2%), with a mean time of 39 months. The overall incidence rate of main thrombotic events was 4.36 per 100 patient-years. The incidence of arterial hypertension (HTA) was significantly higher in patients with thrombosis, compared to those without thrombosis (P <.05). The level of soluble P-selectin was significantly higher in patients with thrombosis compared to those without thrombosis (346.89 ng/mL vs 286.39 ng/mL, P =.034). The mean level of Neu-Plt (26.7% vs 22.4%) and Mo-Plt (17.8% vs 12.3%) aggregates did not differ significantly between the groups with and without thrombosis. A multivariate COX proportional hazard regression model confirmed an independent predictive significance of Mo-Plt aggregates (HR = 1.561, 95% CI: 1.007-2.420, P =.046), as well as the cumulative effect of Mo-Plt aggregates and HTA (HR = 1.975, 95%CI: 1.215-3.212, P =.006) for thrombosis occurrence. Conclusion: Monocyte-platelet aggregates represent an independent risk factor for thrombosis occurrence, further on supported by HTA. © 2021 John Wiley & Sons Ltdmonocytesmyeloproliferative neoplasmsplatelet activationselectinsthrombosis