Stamenkovic, Miroslav (7003436370)Miroslav (7003436370)StamenkovicLukic, Vesna (57353220400)Vesna (57353220400)LukicSuvakov, Sonja (36572404500)Sonja (36572404500)SuvakovSimic, Tatjana (6602094386)Tatjana (6602094386)SimicSencanic, Ivan (55376191500)Ivan (55376191500)SencanicPljesa-Ercegovac, Marija (16644038900)Marija (16644038900)Pljesa-ErcegovacJaksic, Vesna (23667666000)Vesna (23667666000)JaksicBabovic, Sinisa (57384231300)Sinisa (57384231300)BabovicMatic, Marija (58618962300)Marija (58618962300)MaticRadosavljevic, Aleksandra (56993158000)Aleksandra (56993158000)RadosavljevicSavic-Radojevic, Ana (16246037100)Ana (16246037100)Savic-RadojevicDjukic, Tatjana (36193753800)Tatjana (36193753800)Djukic2025-06-122025-06-122018https://doi.org/10.18240/ijo.2018.09.14https://www.scopus.com/inward/record.uri?eid=2-s2.0-85053377610&doi=10.18240%2fijo.2018.09.14&partnerID=40&md5=8d4423665eb039f602254dd2c5583b9dhttps://remedy.med.bg.ac.rs/handle/123456789/6575Aim: To evaluate glutathione transferase theta 1 and mu 1 (GSTT1 and GSTM1) polymorphisms as determinants of primary open angle glaucoma (POAG) risk, independently or in combination with cigarette smoking, hypertension and diabetes mellitus. ● Methods: A case-control study with 102 POAG patients and 202 age and gender-matched controls was carried out. Multiplex-polymerase chain reaction method was used for the analysis of GSTM1 and GSTT1 polymorphisms. The differences between two groups were tested by the t-test or χ2test. Logistic regression analysis was used for assessing the risk for disease development. ● Results: The presence of GSTM1-null genotype did not contribute independently towards the risk of POAG. However, individuals with GSTT1-active genotype were at almost two-fold increased risk to develop glaucoma (P=0.044) which increased up to 4.36 when combined with GSTM1-null carriers (P=0.024). When glutathione transferase (GST) genotypes were analyzed in association with cigarette smoking, hypertension and diabetes, only carriers of GSTT1-active genotype had significantly increased risk of POAG development in comparison with GSTT1-null genotype individuals with no history of smoking, hypertension and diabetes, respectively (OR=3.52, P=0.003; OR=10.02, P<0.001; OR=4.53, P=0.002). ● Conclusion: The results obtained indicate that both GSTM1-null and GSTT1-active genotypes are associated with increased POAG risk among smokers, suggesting potential gene-environment interaction in glaucoma development. © 2018, International Journal of Ophthalmology (c/o Editorial Office). All rights reserved.Diabetes mellitusGSTM1GSTT1HypertensionPrimary open angle glaucoma riskSmoking