Stojanovic, M. (58191563300)M. (58191563300)StojanovicPopevic, M. (35732042800)M. (35732042800)PopevicPekic, S. (6602553641)S. (6602553641)PekicDoknic, M. (6603478362)M. (6603478362)DoknicMiljic, D. (6505968542)D. (6505968542)MiljicMedic-Stojanoska, M. (23389630200)M. (23389630200)Medic-StojanoskaTopalov, D. (7801389703)D. (7801389703)TopalovStojanovic, J. (58823726400)J. (58823726400)StojanovicMilovanovic, A. (57206496112)A. (57206496112)MilovanovicPetakov, M. (7003976693)M. (7003976693)PetakovDamjanovic, S. (7003775804)S. (7003775804)DamjanovicPopovic, V. (35451450900)V. (35451450900)Popovic2025-06-122025-06-122021https://doi.org/10.4183/aeb.2021.462https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135390687&doi=10.4183%2faeb.2021.462&partnerID=40&md5=b9cba0549a5361d7278b10004a35706ahttps://remedy.med.bg.ac.rs/handle/123456789/3998Context. Insulin-like growth factor-1 (IGF-1) is main serum surrogate marker of growth hormone (GH) secretion, used in diagnostics and treatment of GH deficiency (GHD) and acromegaly. Regional, ethnic, racial or nutritional factors obscure cross-population applicability of IGF-1 reference values. Establishment of population-and assay-specific reference values requires sizable representative cohort of healthy subjects. Subjects and Methods. In representative sample of healthy adult population of Serbia (N=1200, 21-80 years, 1:1 male:female) serum IGF-1 was analyzed by Siemens Immulite 2000 assay under uniform laboratory conditions. Upper and lower limit of reference range (5th-95th percentile) were calculated for each of the 12 quinquennial age intervals. IGF-1 distribution was normalized and standard deviation score (SDS) calculated by Logarithmic and LMS methods. Results. IGF-1 and age correlated significantly, with most prominent decline at 21-50 years, followed by a plateau up to age of 70. Gender differences were not significant overall. Plateau in age-related IGF-1 decline was less prominent in women. Correlations of IGF-1 with body mass index (BMI) or waist to hip ratio (WHR) were insignificant. Superior IGF-1 SDS transformation was achieved with LMS method, while logarithmic method was simpler to use. Conclusions. Normative age-specific serum IGF-1 reference values were established on a representative cohort of healthy adults in Serbia. Our results support recommendations against necessity for gender-specific or BMI-and WHR-specific reference ranges. Population-based data serve to generate IGF-1 SDS, which is valuable in rational application of consensus guidelines, proper longitudinal follow-up, advancement in efficacy and safety and personalization of treatment targets. © 2021, Acta Endocrinologica Foundation. All rights reserved.Growth hormoneIGF-1normal healthy populationnormative datapopulation-basedSD-scoreSoutheast Europe