Vuletić, Ana (16243138800)Ana (16243138800)VuletićJovanić, Irena (55623723900)Irena (55623723900)JovanićJurišić, Vladimir (6603015144)Vladimir (6603015144)JurišićMilovanović, Zorka (25228841900)Zorka (25228841900)MilovanovićNikolić, Srđan (56427656200)Srđan (56427656200)NikolićSpurnić, Igor (56613372800)Igor (56613372800)SpurnićKonjević, Gordana (56008692300)Gordana (56008692300)Konjević2025-06-122025-06-122015https://doi.org/10.1007/s12253-015-9938-3https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940447235&doi=10.1007%2fs12253-015-9938-3&partnerID=40&md5=1de1e0498a30b5cb8ac5dfa8b23f29c3https://remedy.med.bg.ac.rs/handle/123456789/8148As lymphogenic dissemination is very common in melanoma, regional lymph nodes (LN)s represent first immunological barriers to tumor invasion and play a complex role in antitumor immune defense. In this sense, their most prominent role is the presentation of tumor-derived antigens to naïve T cells and generation of cell-mediated adaptive immune response. Since tumor micro-environment affects immune cell function in this study we have evaluated the ability of T cells and NK cells in metastatic (involved) and non-metastatic regional LNs to produce interferon γ (IFNγ), a pleiotropic cytokine that regulates adaptive antitumor immune response. Our results show reduced IFNγ production in both T and NK lymphocyte subsets and decreased prevalence of T cells in metastatic regional LNs of melanoma patients. The decrease of IFNγ production in T cells was more pronounced with increased number of involved regional LNs indicating tumor-induced functional impairment of both T and NK cell lymphocyte subsets in involved regional LNs. Therefore, shown low IFNγ production in metastatic LNs may represent an obstacle in adaptive cell-mediated antitumor immune response and hence may enable tumor progression. © 2015, Arányi Lajos Foundation.Interferon γNK cellsRegional lymph nodesT cells