Jovanović, Milica (55305700000)Milica (55305700000)JovanovićBožić, Milica (36624858100)Milica (36624858100)BožićKovačević, Tamara (36899452000)Tamara (36899452000)KovačevićRadojčić, Ljiljana (8318717000)Ljiljana (8318717000)RadojčićPetronijević, Miloš (21739995200)Miloš (21739995200)PetronijevićVićovac, Ljiljana (6602991242)Ljiljana (6602991242)Vićovac2025-07-022025-07-022010https://doi.org/10.1016/j.acthis.2008.07.001https://www.scopus.com/inward/record.uri?eid=2-s2.0-73849089188&doi=10.1016%2fj.acthis.2008.07.001&partnerID=40&md5=390bab9000e05312659c9d7bc5d72871https://remedy.med.bg.ac.rs/handle/123456789/14202Antibodies to phospholipids (aPL) have been shown to adversely affect trophoblast invasion in vivo and in vitro. HTR-8/SVneo cells derived from first trimester of pregnancy extravillous trophoblast were studied. Matrigel invasion assay, cytochemistry and cell-based enzyme-linked immunosorbant assay (ELISA) with aPL or normal IgG was used. Our data show that aPL at 100 μg/ml decrease invasiveness of HTR-8/SVneo cells to 60% of control (p<0.01), and this was also shown for primary cytotrophoblast (to 15.5% of control, p<0.001). aPL treatment caused a significant decrease in integrin α1, α5, and β1 proteins (86%, 84%, and 87%, respectively). We conclude that HTR-8/SVneo cell culture is a suitable model to study mechanisms of action of aPL on trophoblast, which in HTR-8/SVneo cells inhibit invasion by decreasing integrins α5, α1, and β1. © 2008 Elsevier GmbH. All rights reserved.Anti-phospholipid syndromeExtravillous trophoblastHTR-8/SVneoHumanIntegrins