Toljic, Mina (57194077869)Mina (57194077869)ToljicJoksic, Ivana (14054233100)Ivana (14054233100)JoksicNikolic, Nadja (55324775800)Nadja (55324775800)NikolicCarkic, Jelena (55802211000)Jelena (55802211000)CarkicMilasin, Jelena (6603015594)Jelena (6603015594)MilasinMunjas, Jelena (57194078742)Jelena (57194078742)MunjasKaradzov Orlic, Natasa (41561546900)Natasa (41561546900)Karadzov Orlic2025-06-122025-06-122024https://doi.org/10.1016/j.ejogrb.2024.07.069https://www.scopus.com/inward/record.uri?eid=2-s2.0-85200388278&doi=10.1016%2fj.ejogrb.2024.07.069&partnerID=40&md5=cfbcee9530e146f0c5473070cbbd8f6bhttps://remedy.med.bg.ac.rs/handle/123456789/880Objectives: Gestational hypertension (GH) is one of the most common pregnancy-related complications, however, there is still insufficient knowledge about its development and molecular changes. The aim of our study was to examine the expression of miR-17, miR-29a and miR-181a, as well as TNF-α, IL-1β, IL-6 and IL-17 in women with GH and to investigate possible correlations between these parameters. Study design: The study included 64 pregnant women, placed either in the control or the GH group. Quantitative real-time PCR (qPCR) was used to determine expression levels of microRNAs and cytokines’ mRNAs. Main outcome measures: Expression levels of miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines mRNAs (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational hypertension were compared to the control group (healthy pregnant women). Results: No significant changes in microRNAs expression level were found between compared groups. TNF-α was significantly upregulated in the GH group compared to controls. Expression levels of other investigated cytokines did not differ between examined groups. ROC curve analysis indicated that TNF-α does not show sufficient ability to discriminate between CG and GH patients. TNF-α was significantly positively correlated with IL-1β and IL-17 and negatively correlated with miR-181a. Conclusions: Our results point to the involvement of proinflamatory cytokines in gestational hypertension. Although increased expression of TNF-α was found in the GH group, this cytokine did not show sufficient ability to discriminate between GH and healthy pregnancies. © 2024 Elsevier B.V.BiomarkerGestational hypertensionmicroRNAProinflamatory cytokineTNF-α