Janac, Jelena M. (53874919200)Jelena M. (53874919200)JanacZeljkovic, Aleksandra (15021559900)Aleksandra (15021559900)ZeljkovicJelic-Ivanovic, Zorana D. (6603775254)Zorana D. (6603775254)Jelic-IvanovicDimitrijevic-Sreckovic, Vesna S. (6506375884)Vesna S. (6506375884)Dimitrijevic-SreckovicVekic, Jelena (16023232500)Jelena (16023232500)VekicMiljkovic, Milica M. (55066891400)Milica M. (55066891400)MiljkovicStefanovic, Aleksandra (15021458500)Aleksandra (15021458500)StefanovicKotur-Stevuljevic, Jelena M. (6506416348)Jelena M. (6506416348)Kotur-StevuljevicIvanisevic, Jasmina M. (54389258300)Jasmina M. (54389258300)IvanisevicSpasojevic-Kalimanovska, Vesna V. (6602511188)Vesna V. (6602511188)Spasojevic-Kalimanovska2025-07-022025-07-022021https://doi.org/10.1093/LABMED/LMZ017https://www.scopus.com/inward/record.uri?eid=2-s2.0-85077402988&doi=10.1093%2fLABMED%2fLMZ017&partnerID=40&md5=acb18e6fcbb767d9682e2836513da056https://remedy.med.bg.ac.rs/handle/123456789/12414Background: We evaluated the qualitative characteristics of highdensity lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/ HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals. © American Society for Clinical Pathology 2019. All rights reserved.HDL-cholesterolLipoprotein subfractionsMetabolically healthy phenotypeOverweight and obesityOxidized HDLSerum amyloid A