Toljic, Mina (57194077869)Mina (57194077869)ToljicNikolic, Nadja (55324775800)Nadja (55324775800)NikolicJoksic, Ivana (14054233100)Ivana (14054233100)JoksicCarkic, Jelena (55802211000)Jelena (55802211000)CarkicMunjas, Jelena (57194078742)Jelena (57194078742)MunjasKaradzov Orlic, Natasa (41561546900)Natasa (41561546900)Karadzov OrlicMilasin, Jelena (6603015594)Jelena (6603015594)Milasin2025-06-122025-06-122024https://doi.org/10.1016/j.jri.2024.104211https://www.scopus.com/inward/record.uri?eid=2-s2.0-85184899258&doi=10.1016%2fj.jri.2024.104211&partnerID=40&md5=82eb23a14c9a971da2407fe4321c8af4https://remedy.med.bg.ac.rs/handle/123456789/1240Altered microRNAs (miRNAs1) and cytokines expression levels are associated with several pregnancy-induced complications. We evaluated the profile of circulating miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational diabetes mellitus (GDM2), as well as their potential use as GDM biomarkers. The case-control study included 65 pregnant women divided into 2 groups - GDM and control. Expression levels of miRNAs in plasma samples and cytokines mRNA isolated from peripheral blood buffy coat were analyzed by quantitative real-time PCR (qPCR3). Significant miR-29a downregulation was found in GDM compared to the control group, and was even more significant after adjustments for covariates. miR-17 and miR-181a expression levels did not differ between the examined groups. Expression levels of IL-1β were significantly higher in GDM group compared to controls, while TNF-α, IL-6 and IL-17 did not show significant changes in expression between the two groups. As jugded from the ROC curve analysis, miR-29a and IL-1β had a significant capacity to discriminate between CG and GDM. Additionally, a positive correlation was established between IL-1β and TNF-α in the GDM group. GDM appeared to be associated with altered levels of miR-29a and IL-1β making them markers of this condition. © 2024 Elsevier B.V.Gestational diabetes mellitusIL-1βMicroRNAmiR-29aProinflamatory cytokine