Vidović, Jovana (57218177977)Jovana (57218177977)VidovićStojanović, Dragica (7007127831)Dragica (7007127831)StojanovićCagnardi, Petra (7801584853)Petra (7801584853)CagnardiKladar, Nebojša (55905835500)Nebojša (55905835500)KladarHorvat, Olga (16241449800)Olga (16241449800)HorvatĆirković, Ivana (16309091000)Ivana (16309091000)ĆirkovićBijelić, Katarina (57408280600)Katarina (57408280600)BijelićStojanac, Nenad (55834568300)Nenad (55834568300)StojanacKovačević, Zorana (55889598900)Zorana (55889598900)Kovačević2025-07-022025-07-022022https://doi.org/10.3390/antibiotics11010064https://www.scopus.com/inward/record.uri?eid=2-s2.0-85122675478&doi=10.3390%2fantibiotics11010064&partnerID=40&md5=3799f2c45ce3642c5d254854002a2b5ahttps://remedy.med.bg.ac.rs/handle/123456789/12192Antimicrobial resistance (AMR) is considered one of the most prevalent global health issues in both veterinarian and human medicine. This complex problem requires a “One Health” approach with the cooperation of all healthcare sectors, as well as agriculture, finance, and consumers. We conducted a survey with the objective to assess the knowledge and attitudes of farm animal veterinarians toward AMR and antimicrobial use in the Republic of Serbia with a small focus on mastitis therapy. A total of 110 respondents completed the questionnaire, which represents a response rate of 27.3%. The majority of our respondents (n = 102, 92.7%) completely agreed that AMR currently represents severe concern in the health sector. Unfortunately, less than one-third (n = 34, 30.9%) of the respondents had only heard about antimicrobial stewardship. Participants showed a positive attitude toward prudent antimicrobial use and were open to solutions to the AMR crisis. We noticed a certain gap between farm veterinarians’ desire to improve and perform better in daily practice, while at the same time feeling like they did not have enough guidance, help, and resources. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Antimicrobial resistanceAntimicrobial stewardshipAntimicrobial therapyFarm animalsMastitisVeterinarians