Smitran, Aleksandra (55865631000)Aleksandra (55865631000)SmitranLukovic, Bojana (57189443662)Bojana (57189443662)LukovicBozic, LJiljana (57202649173)LJiljana (57202649173)BozicJelic, Dijana (23034961300)Dijana (23034961300)JelicJovicevic, Milos (57223044336)Milos (57223044336)JovicevicKabic, Jovana (57215669275)Jovana (57215669275)KabicKekic, Dusan (36696225200)Dusan (36696225200)KekicRanin, Jovana (57219407010)Jovana (57219407010)RaninOpavski, Natasa (6507364674)Natasa (6507364674)OpavskiGajic, Ina (55428924700)Ina (55428924700)Gajic2025-07-022025-07-022023https://doi.org/10.3390/microorganisms11010171https://www.scopus.com/inward/record.uri?eid=2-s2.0-85146822373&doi=10.3390%2fmicroorganisms11010171&partnerID=40&md5=36f73174690b98c19d9b32505749f465https://remedy.med.bg.ac.rs/handle/123456789/11891This study aimed to investigate the biofilm-production ability of carbapenem-resistant Acinetobacter baumannii (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against planktonic and biofilm-embedded CRAB, and the relationship between biofilm production and bacterial genotypes. A total of 111 CRAB isolates were tested for antimicrobial susceptibility, biofilm formation, presence of the genes encoding carbapenemases, and biofilm-associated virulence factors. The antibiofilm effects of disinfectants and SeNPs against CRAB isolates were also tested. The vast majority of the tested isolates were biofilm producers (91.9%). The bap, ompA, and csuE genes were found in 57%, 70%, and 76% of the CRAB isolates, with the csuE being significantly more common among biofilm producers (78.6%) compared to non-biofilm-producing CRAB (25%). The tested disinfectants showed a better antibiofilm effect on moderate and strong biofilm producers than on weak producers (p < 0.01). The SeNPs showed an inhibitory effect against all tested planktonic (MIC range: 0.00015 to >1.25 mg/mL) and biofilm-embedded CRAB, with a minimum biofilm inhibitory concentration of less than 0.15 mg/mL for 90% of biofilm producers. In conclusion, SeNPs might be used as promising therapeutic and medical device coating agents, thus serving as an alternative approach for the prevention of biofilm-related infections. © 2023 by the authors.biofilm-associated genescarbapenem-resistant Acinetobacter baumanniidisinfectantsselenium nanoparticlesCarbapenem-Resistant Acinetobacter baumannii: Biofilm-Associated Genes, Biofilm-Eradication Potential of Disinfectants, and Biofilm-Inhibitory Effects of Selenium Nanoparticles