Prokić, Dragan (6603769235)Dragan (6603769235)ProkićĐuričić, Slaviša (22940480000)Slaviša (22940480000)ĐuričićKitić, Ivana (56079613400)Ivana (56079613400)KitićKocić, Marija (57192209334)Marija (57192209334)KocićPašić, Srđan (55904557400)Srđan (55904557400)PašićVuletić, Biljana (25121846900)Biljana (25121846900)Vuletić2025-06-122025-06-122023https://doi.org/10.2298/SARH230113064Phttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85175199948&doi=10.2298%2fSARH230113064P&partnerID=40&md5=487d4ee4cc19858ccad8c74430c97262https://remedy.med.bg.ac.rs/handle/123456789/2967Introduction/Objective The objective of the paper is to assess the applicability of serum anti-tissue transglutaminase (tTG) antibodies IgA and IgG concentration and HLA-DQ2/DQ8 typing as a non-invasive alternative to duodenal biopsy in diagnosing celiac disease (CD) in pediatric population. Methods A prospective cohort study included a total of 179 pediatric patients aged 1–18 years. Determination of tTG IgA and tTG IgG antibodies and human leukocyte antigen (HLA) DQ2/DQ8 typing was performed for all patients. Histology of duodenal biopsies was interpreted by the modified Marsh scoring system. Results The diagnosis of CD was confirmed in 101 (56%) patients of the studied population. In cases of CD, HLA-DQ2/DQ8 was positive in 100 patients (99%). The tTG IgA antibodies in concentration higher than 100 U/ml were detected in 77 (76.2%) of the CD patients and in significantly smaller number for tTG IgG [29 (28.7%)] (p < 0.001). Statistically highly significant association of duodenal lesions Marsh grade 3 with concentration of tTG IgA 10-fold higher than the upper level of normal (ULN) was established (p < 0.001) Conclusion Concentration of tTG IgA 10-fold higher than ULN is significantly positively correlated with Marsh grade 3 histopathology findings. Specific antibodies determination in combination with HLA-DQ2/ DQ8 typing proves to be sufficient for a diagnosis of CD, supporting the fact that duodenal biopsy may be avoided in a significant majority of patients – 75%. © 2023, Serbia Medical Society. All rights reserved.celiac diseaseHLA-DQ2/DQ8 typingnon-invasivetissue transglutaminase antibodies