Folic, Miljan M. (56497240500)Miljan M. (56497240500)FolicBanko, Ana V. (35774145100)Ana V. (35774145100)BankoTodorovic, Vera N. (7006326762)Vera N. (7006326762)TodorovicPuskas, Nela S. (15056782600)Nela S. (15056782600)PuskasMilovanovic, Jovica P. (6603250148)Jovica P. (6603250148)MilovanovicKrejovic, Sanja B. (57995602200)Sanja B. (57995602200)KrejovicDragicevic-Babic, Neda Z. (57995555700)Neda Z. (57995555700)Dragicevic-BabicBukumiric, Zoran M. (36600111200)Zoran M. (36600111200)BukumiricMilicic, Biljana R. (6603829143)Biljana R. (6603829143)MilicicJotic, Ana D. (35173257500)Ana D. (35173257500)JoticDjukic, Vojko B. (6701658274)Vojko B. (6701658274)Djukic2025-06-122025-06-122022https://doi.org/10.1177/10732748221144457https://www.scopus.com/inward/record.uri?eid=2-s2.0-85143459212&doi=10.1177%2f10732748221144457&partnerID=40&md5=2ceee67cfa1c40464761bb05b9e9ecddhttps://remedy.med.bg.ac.rs/handle/123456789/3709Introduction: The association between the expression of HIF-1α in the laryngeal carcinoma and the prognosis of disease is quite well documented, but the significance of HIF-1α C1772T polymorphism and its relation to disease phenotype have to be clarified. The aim of this study was to investigate the influence of C1772T polymorphism on the clinical-pathological characteristics and disease-free survival after initial surgical treatment of patients with laryngeal carcinoma. Materials and methods: The prospective cohort study included 65 patients with laryngeal carcinoma. Two representative tumor tissue specimens were taken in each patient during surgery; 1 specimen was used to asses HIF-1α C1772T polymorphism and the other 1 to determine the immunohistochemical expression of HIF-1α, VEGF, as well as CD 34 proteins. The comparison of polymorphism frequency between study and control population was conducted by collecting a 5 mL of peripheral venous blood samples in each subject. Results: Clinicopathological characteristics of laryngeal carcinoma didn’t affect the expression of hypoxia-related biomarkers, such as HIF-1α, VEGF or MVD. The statistically significant association between HIF-1α and VEGF expression was found (P =.034), but not between HIF-1α expression and MVD value (P =.696). The expression of HIF-1α was significantly higher among CT heterozygotes (P =.029). We found a significantly more recurrence among CT heterozygotes compared with patients with CC homozygous alleles (57.10% and 24.30%, respectively; P =.007). Patients with C1772T polymorphic variants had significantly worse disease-free survival compared with patients without polymorphism (Log-rank test, P =.007). Conclusion: HIF-1α C1772T polymorphism was significantly associated with worse disease-free survival which nominates it as a predictor of laryngeal carcinoma relapse. The preoperative assessment of hypoxia-related biomarkers should be used in everyday practice in order to determine the treatment modalities for laryngeal carcinoma. © The Author(s) 2022.hypoxia-inducible factor 1laryngeal squamous cell carcinomamicrovascular densitypolymorphismrecurrencevascular endothelial growth factor