Trajković, Vladimir (7004516866)Vladimir (7004516866)TrajkovićBadovinac, Vladimir (6603057711)Vladimir (6603057711)BadovinacJanković, Vladimir (8277787100)Vladimir (8277787100)JankovićStojković, Mostarica Marija (57213949014)Mostarica Marija (57213949014)Stojković2025-06-122025-06-121999https://doi.org/10.1016/S0006-8993(98)01130-5https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033573754&doi=10.1016%2fS0006-8993%2898%2901130-5&partnerID=40&md5=44fc9e20e2c41e6954831adc13971572https://remedy.med.bg.ac.rs/handle/123456789/1479The effects of immunosuppressant cyclosporin A (CsA) on nitric oxide (NO) production and inducible NO synthase (iNOS) mRNA expression in rat C6 glioma cell line were investigated. CsA applied simultaneously with iNOS activator IFN-γ caused dose-dependent reduction of NO synthesis in confluent C6 cells, as determined by measuring accumulation of nitrite, an indicator of NO production, in 48 h culture supernatants. IFN-γ-induced expression of iNOS, but not interferon regulatory factor-1 (IRF-1) mRNA was reduced in CsA- treated cells. The enzymatic activity of iNOS was not changed by CsA, since it failed to affect NO production in cells in which iNOS had already been induced with IFN-γ and any further induction was blocked by protein synthesis inhibitor cycloheximide (CHX). FK506 was not able to mimic inhibitory effect of CsA on NO production in C6 cells, suggesting calcineurin-independent mechanism of CsA action.C6Cyclosporin AFK506GliomaiNOSNitric oxideRat