Jovanovié, Aleksandar (7006571994)Aleksandar (7006571994)JovanoviéGrbovié, Leposava (36516602700)Leposava (36516602700)GrboviéTulić, Ivan (6602743219)Ivan (6602743219)Tulić2025-06-122025-06-121994https://doi.org/10.1093/oxfordjournals.humrep.a138514https://www.scopus.com/inward/record.uri?eid=2-s2.0-0028327205&doi=10.1093%2foxfordjournals.humrep.a138514&partnerID=40&md5=c5e59d0f0d4c32f32bd044559e504340https://remedy.med.bg.ac.rs/handle/123456789/1763The effect of acetylcholine on the isolated human uterine artery rings was investigated. Acetylcholine (10-10 M to 6× 10-5 M) induced concentration- and endothelium-dependent relaxation (pD2=7.4±0.02, maximal response was 77.5±6.3% of relaxation induced by papaverine at 3×10-4 M), diethylcarbamazine (10∼4 M) and tetra-ethylammonium (3×10-4 M) had no effects on acetylcholine-evoked relaxation. Methylene blue (10-5 M) and NG-monomethyl-L-arginine (L-NMMA) (3×10-6 to 3× 10-5 M) antagonized relaxation induced by acetylcholine. The inhibition of endothelium-dependent relaxation by L-NMMA (10-5 M) was reversed by L-arginine (10-5 M) but not by D-arginine (10-4 M). It is concluded that in uterine artery acetylcholine induces endothelium-dependent relaxation. It is suggested that the acetylcholine-induced relaxation of isolated uterine artery is probably mediated via endothelial nitric oxide formation © 1994 Oxford University Press.AcetylcholineNitric oxideUterine artery